[1]唐映华,杨 兰,莫武宁.脂联素基因rs2241766 多态性与代谢综合征的相关性研究[J].现代检验医学杂志,2020,35(01):5-8.[doi:10.3969/j.issn.1671-7414.2020.01.002]
　TANG Ying-hua,YANG Lan,MO Wu-ning.Association Study between the Polymorphism of Adiponectin Gene rs2241766 and Metabolic Syndrome[J].Journal of Modern Laboratory Medicine,2020,35(01):5-8.[doi:10.3969/j.issn.1671-7414.2020.01.002]
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脂联素基因rs2241766 多态性与代谢综合征的相关性研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第35卷
期数:
2020年01期
页码:
5-8
栏目:
论　　著
出版日期:
2020-02-29

文章信息/Info

Title:
Association Study between the Polymorphism of Adiponectin Gene rs2241766 and Metabolic Syndrome
文章编号:
1671-7414(2020)01-005-04
作者:
唐映华1; 3; 杨 兰2; 莫武宁1
(1.广西医科大学,南宁 530021; 2.南宁市第二人民医院,南宁 530031; 3.广西中医药大学第一附属医院,南宁 530023)
Author(s):
TANG Ying-hua 1; 3;  YANG Lan 2;  MO Wu-ning 1
(1.Guangxi University of Medicine, Nanning 530021,China; 2. the Second People's Hospital of Nanning, Nanning 530031,China; 3.the First Affiliated Hospital of Guangxi University of Traditional Chinese Medicine,Nanning 530023,China)
关键词:
脂联素;  基因多态性;  代谢综合征
分类号:
Q786; R58
DOI:
10.3969/j.issn.1671-7414.2020.01.002
文献标志码:
A
摘要:
目的 探讨脂联素(adiponectin,APN)rs2241766基因多态性与代谢综合征(metabolic syndrome,MS)患者发生的关系。方法 410例MS患者和203例健康人群纳入研究。使用酶联免疫吸附试验(ELISA)检测研究对象的血清APN水平,采用限制性片段长度多态性聚合酶链反应(PCR-RFLP)技术检测APN基因rs2241766 位点T/G基因的多态性,分析各基因型与MS发生的关系。结果 MS患者血清APN水平明显低于对照组(15.3 ± 4.4 mg/ml vs 27.2 ± 6.5 mg/ml),差异有统计学意义(χ2=41.7,P< 0.001); rs2241766位点三种基因型在MS组与对照组间的分布,差异有统计学意义(χ2=39.222,P< 0.001),MS组G等位基因频率高于健康人群,差异有统计学意义(χ2=36.657,P< 0.001); logistic回归分析显示研究组G等位基因发生MS的风险高于T等位基因(OR=2.19, 95% CI:1.17～3.01 vs OR=1.00ref),差异有统计学意义(P=0.034,<0.05)。TG和GG基因型发生MS的风险较TT基因型增高(OR=1.55, 95% CI:0.79～2.83; OR=2.48, 95% CI:1.67～7.35 vs OR=1.00ref),将TG和GG基因型合并后发生MS风险同样增高(OR=2.23,95% CI:1.21～6.09),差异均有统计学意义(P=0.019,0.006,0.029),且TG(15.7± 4.1 mg/ml)及GG(14.3 ± 4.5 mg/ml)基因型研究对象的APN水平低于TT基因型(18.7± 4.9 mg/ml),差异有统计学意义(F=7.621,P< 0.001)。结论 MS的发生可能与rs2241766位点的基因变异相关,尤其是T向G变异的个体发生MS的风险更高,使APN水平降低。
Abstract:
Keywords: Objective To investigate the association between adiponectin(APN)rs2241766 gene polymorphism and patients with metabolic syndrome(MS). Methods 410 patients with MS and 203 healthy subjects were included in the study. The serum APN level of the subjectswas detected by enzyme-linked immunosorbent assay(ELISA), and the polymorphism of APN gene locus rs2241766 T/G was detected by polymerase chain reaction-restriction fragmentlength polymorphism(PCR-RFLP), analyzing the relationship that occurs withMS. Results Serum APN levels in the MS group were significantly lower than those in the control group(15.3 ± 4.4 mg/ml vs 27.2 ± 6.5 mg/ml),the difference was statistically significant(t=41.7,P< 0.001). The distribution of the three genotypes of rs2241766 between MS patients and the control group were statistically significant(χ2=39.222, P<0.001), and the G allele frequency of MS patients was higher than healthy population,the difference was statistically significant(χ2=36.657, P< 0.001). Logistic regression analysis showed that the risk of MS in the G allele was higher than that in the T allele(OR=2.19, 95% CI:1.17～3.01 vs OR=1.00ref), the difference was statistically significant(P=0.034),and the risk of MS in TG and GG genotypes was higher than that in the TT genotype(OR=1.55, 95% CI:0.79～2.83; OR=2.48, 95% CI:1.67～7.35 vs OR=1.00ref),the risk of MS was still increased after the combination of TG and GG genotypes(OR=2.23,95% CI:1.21～6.09), the difference was statistically significant(P=0.019,0.006,0.029). And the APN levels of the TG(15.7± 4.1 mg/ml)and GG(14.3 ± 4.5 mg/ml)subjects were lower than those of the TT(18.7± 4.9 mg/ml),the difference was statistically significant(F=7.621,P< 0.001). Conclusion The occurrence of MS may be related to the site variation of rs2241766,especially the individuals with T-to-G variation have higher risk of MS and lower APN level.

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备注/Memo

备注/Memo:
基金项目:广西高校中青年教师科研基础能力提升项目(2019KY0149),广西壮族自治区卫生和计划生育委员会课题(Z20170797)。 作者简介:唐映华(1986-),女,硕士,主管技师,研究方向:分子生物及免疫学。E-mail: 271101521@qq.com 。 通讯作者:莫武宁,女,医学博士,E-mail: mown16300@126.com。 收稿日期:2019-11-12 修回日期:2019-11-29
更新日期/Last Update: 2020-03-30