[1]王 磊,刘 瑞,孙文利,等.HPLC-MS/MS 法在检测慢性粒细胞白血病患者帕纳替尼血药浓度中的应用[J].现代检验医学杂志,2020,35(02):39-42.[doi:10.3969 / j.issn.1671-7414.2020.02.012]
 WANG Lei,LIU Rui,SUN Wen-li,et al.Application of HPLC-MS/MS in the Detection of Blood Concentration ofPonatinib in Patients with Chronic Myelogenous Leukemia[J].Journal of Modern Laboratory Medicine,2020,35(02):39-42.[doi:10.3969 / j.issn.1671-7414.2020.02.012]
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HPLC-MS/MS 法在检测慢性粒细胞白血病患者帕纳替尼血药浓度中的应用()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第35卷
期数:
2020年02期
页码:
39-42
栏目:
论 著
出版日期:
2020-05-24

文章信息/Info

Title:
Application of HPLC-MS/MS in the Detection of Blood Concentration ofPonatinib in Patients with Chronic Myelogenous Leukemia
文章编号:
1671-7414(2020)02-039-05
作者:
王 磊1 刘 瑞1 孙文利1刘红星123
(1. 河北燕达陆道培医院病理和医学检验科,河北 廊坊065201;2. 北京陆道培血液病研究院 ,北京100176;3. 北京陆道培医院病理和医学检验科,北京100176)
Author(s):
WANG Lei1 LIU Rui1 SUN Wen-li1 LIU Hong-xing123
(1. Department of Pathology & Medical Clinical Laboratory, Hebei Yanda Lu Daopei Hospital,China; 2. Beijing Lu Daopei Institute of Hematology, Beijing 100176, China;3. Department of Pathology & Medical Clinical Laboratory, Beijing Lu Daopei Hospital, Be
关键词:
帕纳替尼高效液相色谱- 串联质谱法慢性粒细胞性白血病治疗药物监测
分类号:
R557.3;Q503
DOI:
10.3969 / j.issn.1671-7414.2020.02.012
文献标志码:
A
摘要:
目的 建立测定慢性粒细胞白血病(chronic myelogenous leukemia, CML) 患者血浆帕纳替尼浓度的高效液相色谱- 串联质谱法(HPLC-MS/MS),并应用于帕纳替尼血药浓度日常监测,为帕纳替尼合理使用提供实验室依据。方法 采用含内标(帕纳替尼-d8)的甲醇对患者血浆进行沉淀蛋白处理。色谱柱为Ultimate XB-C18,柱温60℃,流动相为甲醇相(0.1% 甲酸)和水相(0.1% 甲酸和2 mmol/L 乙酸铵),梯度洗脱。质谱检测方式为电喷雾离子阱正离子模式,MRM 扫描,同时监测帕纳替尼m/z 533.1>260.1 和帕纳替尼-d8 m/z 541.2>260.1。采用内标法定量,以帕纳替尼和帕纳替尼-d8 峰面积比为定量依据,计算血浆帕纳替尼浓度,并分别对其线性、专属性、精密度、准确度及稳定性进行考察。结果 帕纳替尼浓度在(1 ~ 250) ng/ml 范围内与峰面积线性关系良好,Y = 0.019 3X + 0.028 4 (r= 0.999 1)。专属性较好,血浆中的内源性物质不干扰帕纳替尼及其内标的测定;日内及日间RSD 均小于5%;相对回收率为100.91%~105.18%;室温放置稳定性及冻融稳定性RSD 均小于5%。结论 该文建立的HPLC-MS/MS 法,前处理简单,特异度及灵敏度高,能准确、快速地检测血浆帕纳替尼浓度,适合慢性粒细胞白血病患者帕纳替尼血药浓度的日常监测。

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备注/Memo

备注/Memo:
作者简介:王磊(1984-),男,硕士,助理研究员,主要从事医院药学研究工作,E-mail:rosie1982@163.com。通讯作者:刘红星,男,医学硕士,副研究员,主要从事检验医学研究工作。收稿日期:2019-10-11修回日期:2019-11-07
更新日期/Last Update: 2020-04-30