[1]周淑燕,柳丽娟,卓传尚,等.梅里埃MALDI-TOF-MS 系统与VITEK 2 Compact 全自动微生物鉴定仪对临床常见病原菌鉴定的一致性分析[J].现代检验医学杂志,2020,35(02):92-96.[doi:10.3969/j.issn.1671-7414.2020.02.026]
 ZHOU Shu-yan,LIU Li-juan,ZHUO Chuan-shang,et al.Consistency Analyzer for Identification of Common Clinical PathogenicBacteria by the Merrier MALDI-TOF-MS System and VITEK 2 Compact[J].Journal of Modern Laboratory Medicine,2020,35(02):92-96.[doi:10.3969/j.issn.1671-7414.2020.02.026]
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梅里埃MALDI-TOF-MS 系统与VITEK 2 Compact 全自动微生物鉴定仪对临床常见病原菌鉴定的一致性分析()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第35卷
期数:
2020年02期
页码:
92-96
栏目:
研究简报·实验技术
出版日期:
2020-05-24

文章信息/Info

Title:
Consistency Analyzer for Identification of Common Clinical PathogenicBacteria by the Merrier MALDI-TOF-MS System and VITEK 2 Compact
文章编号:
1671-7414(2020)02-092-05
作者:
周淑燕柳丽娟卓传尚吴绍贵李圣聪冯惠娟
( 福建医科大学孟超肝胆医院/ 福州市传染病医院检验科,福州 350025)
Author(s):
ZHOU Shu-yan LIU Li-juan ZHUO Chuan-shang WU Shao-gui LI Sheng-congFENG Hui-juan
(Deartment of Clinical Laboratory, Fujian Medical University Mengchao Hepatobiliary Hospital /Fuzhou InfectiousDisease Hospital, Fuzhou 350025, China)
关键词:
病原菌鉴定基质辅助激光解吸电离飞行时间质谱VITEK 2 Compact
分类号:
R446
DOI:
10.3969/j.issn.1671-7414.2020.02.026
文献标志码:
A
摘要:
目的 分析梅里埃基质辅助激光解吸电离飞行时间质谱(matrix-assisted laser desorption ionization-time of flightmass spectrometry,MALDI-TOF-MS)系统与VITEK 2 Compact 全自动微生物鉴定仪对临床常见病原菌鉴定的一致性。方法 收集福建医科大学孟超肝胆医院2018 年6 月~ 2019 年3 月来自临床不同标本分离的非重复菌株300 株,分别采用梅里埃MALDI-TOF-MS 系统和VITEK 2 Compact 全自动微生物鉴定仪对病原菌进行鉴定,结果不一致菌株采用16SrDNA 基因测序予以确认,比较两种鉴定方法的准确性与一致性,评估两种方法在鉴定16 株大肠埃希菌的时间和经济成本。结果 300 株临床分离菌中,鉴定到种水平的菌株MALDI-TOF-MS 有268 株(89.33%),VITEK 2 Compact 有266 株(88.67%);鉴定到属水平的菌株MALDI-TOF-MS 有297 株(99.00%),VITEK 2 Compact 有297 株(99.00%);两种方法在种属鉴定的一致性分别为82.67% 与98.33%,MALDI-TOF-MS 的准确性略高于VITEK 2 Compact。两种方法鉴定不一致菌株有39 株(13.00%),与16SrDNA 测序鉴定结果相比,MALDI-TOF MS 的准确率(79.49%) 高于VITEK 2Compact(58.97%)。对16 株大肠埃希菌的鉴定,MALDI-TOF-MS 耗时较VITEK 2 Compact 少3.5h,且成本仅为VITEK2 Compact 的2/5。结论 MALDI-TOF MS 与VITEK 2 Compact 对于临床常见病原菌的鉴定结果较为一致,但MALDITOFMS 准确率更高,且更快速与经济。

参考文献/References:

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备注/Memo

备注/Memo:
基金项目:福州市卫生计生中青年科学研究项目(2018-S-wq27),福州市感染性疾病医学中心(2018080306)。作者简介:周淑燕(1989-), 女, 本科, 检验师, 主要从事临床微生物检验工作,E-mail:zshuyan123@163.com。收稿日期:2019-11-26 修回日期:2020-01-02
更新日期/Last Update: 2020-04-30