[1]林　彤,廖贤平.男性不育症患者精浆中miR-145 的表达水平及其临床意义[J].现代检验医学杂志,2020,35(03):39-41.[doi:10.3969/j.issn.1671-7414.2020.03.009]
　LIN Tong,LIAO Xian-ping.Expression of miR-145 in Seminal Plasma of Male Infertility Patients and Its Clinical Significance[J].Journal of Modern Laboratory Medicine,2020,35(03):39-41.[doi:10.3969/j.issn.1671-7414.2020.03.009]
点击复制

男性不育症患者精浆中miR-145 的表达水平及其临床意义()
分享到：

《现代检验医学杂志》[ISSN:/CN:]

卷:
第35卷
期数:
2020年03期
页码:
39-41
栏目:
论著
出版日期:
2020-07-20

文章信息/Info

Title:
Expression of miR-145 in Seminal Plasma of Male Infertility Patients and Its Clinical Significance
文章编号:
1671-7414（2020）03-039-04
作者:
林　彤; 廖贤平
（广州东仁医院生殖医学科，广州　510440）
Author(s):
LIN Tong;  LIAO Xian-ping
（Department of Reproductive Medicine，Guangzhou Dongren Hospital，Guangzhou 510440，China）
关键词:
男性不育症; 微小核糖核酸-145
分类号:
R698.2；R392.11
DOI:
10.3969/j.issn.1671-7414.2020.03.009
摘要:
目的　探讨男性不育症患者精浆中miR-145 的表达水平及其临床意义。方法　招募2017 年1 月~ 2018 年9 月 男性不育症患者130 例和同期体检正常的生育男性50 例为正常对照组。采用实时荧光定量PCR 检测精浆中miR-145 的 表达水平，精液常规参数采用WLJY-9000 伟力彩色精子检测系统进行分析。应用ROC 曲线分析miR-145 对男性不育症 的诊断价值，采用Pearson 相关分析精浆中miR-145 表达水平与精液参数的相关性。结果　男性不育症组精浆中miR- 145 表达水平明显高于对照组（3.92±0.86 vs 1.16±0.35），而男性不育症组精子浓度（70.24±28.60 vs 112.50±46.35） ×106/mL、精子存活率（38.40±7.60 vs 73.84±5.82）%、前向运动精子百分率（17.52±9.73 vs 46.20±5.30）% 及正常 精子形态百分率（2.60±1.42 vs 9.25±1.70）% 明显低于对照组，差异均有统计学意义（t=4.852~11.238，均P<0.01）。 ROC 曲线分析显示，精浆中miR-145 表达水平诊断男性不育症的曲线下面积（AUC）为0.864（95%CI：0.807~0.925）， 其最佳诊断截断值为2.15，敏感度和特异度分别为92.8% 和75.0%。相关分析显示，男性不育症患者精浆中miR-145 表 达水平与精子浓度、精子存活率、前向运动精子百分率均呈正相关（r=0.427，0.604，0.538，均P<0.01）。结论　精浆 中miR-145 表达水平在男性不育症患者中明显上调，且精子浓度、精子存活率、前向运动精子百分率呈正相关，有望 作为男性不育症的辅助诊断指标。
Abstract:
Objective　To investigate the expression of miR-145 in seminal plasma of male infertility patients and its clinical significance. Methods　130 male infertility patients and 50 fertile males with normal physical examination as normal control group from January 2017 to September 2018 were recruited. Real-time fluorescence quantitative PCR was used to detect the expression of miR-145 in seminal plasma. The conventional parameters of semen were analyzed by WLJY-9000 Weili color sperm detection system. ROC curve was used to analyze the diagnostic value of miR-145 in male infertility. Pearson correlation was used to analyze the correlation between miR-145 expression level in seminal plasma and semen parameters. Results　The expression level of miR- 145 in seminal plasma of male infertility group was significantly higher than that of control group（3.92±0.86 vs 1.16±0.35）. The sperm concentration （70.24±28.60 vs 112.50±46.35）×106/ml, sperm survival rate （38.40±7.60 vs 73.84±5.82）%, forward motility sperm percentage （17.52±9.73 vs 46.20±5.30）% and normal sperm morphology percentage （2.60±1.42 vs 9.25±1.70）% in male infertility group were significantly lower than those in control group, the differences were not stantisically significant (t=4.852~11.238, all P<0.01). ROC curve analysis showed that the area under the curve (AUC) of seminal plasma miR-145 expression level in diagnosis of male infertility was 0.864 (95% CI: 0.807~ 0.925). The best diagnostic truncation value was 2.15, and the sensitivity and specificity were 92.8% and 75.0%, respectively. The correlation analysis showed that the expression level of miR-145 in seminal plasma of male infertility patients was positively correlated with sperm concentration, sperm survival rate and forward motile sperm percentage（r=0.427, 0.604, 0.538, all P<0.01）. Conclusion　The expression of miR-145 in seminal plasma was significantly up-regulated in male infertility patients, and there was a positive correlation among sperm concentration, sperm survival rate and percentage of forward motile sperm, which was expected to be used as an auxiliary diagnostic index for male infertility.

参考文献/References:

[1]　MEHRA B L, SKANDHAN K P, PRASAD B S, et al. Male infertility rate: a retrospective study[J]. Urologia, 2018, 85(1): 22-24.
[2]　YUMURA Y, TSUJIMURA A, IMAMOTO T, et al. Nationwide survey of urological specialists regarding male infertility: results from a 2015 questionnaire in Japan[J]. Reprod Med Biol, 2017, 17(1): 44-51.
[3]　ZHANG L, DING Xian, NIE Shuangshuang, et al. Association of hsa-miR-145 overexpression in human testicular cells with male infertility[J]. Mol Med Rep, 2015, 11(6): 4365-4372.
[4]　PROC?PIO M S, DE AVELAR G F, COSTA G M J, et al. MicroRNAs in sertoli cells: implications for spermatogenesis and fertility[J]. Cell Tissue Res, 2017, 370(3): 335-346.
[5]　FLANNIGAN R, SCHLEGEL P N. Genetic diagnostics of male infertility in clinical practice[J]. Best Pract Res Clin Obstet Gynaecol, 2017, 44(10): 26-37.
[6]　YU Hianying, SUN Jianying, JIANG Shaochang, et al. MicroRNA-490-3p regulates cell proliferation and apoptosis in gastric cancer via direct targeting of AKT1[J]. Exp Ther Med, 2019, 17(2): 1330-1336.
[7]　　ROBINSON C L, ZHANG Lingna, SCH?TZ L F, et al. MicroRNA 221 expression in theca and granulosa cells: hormonal regulation and function[J]. J Anim Sci, 2018, 96(2): 641-652.
[8]　　LI Cailing, YANG Bo, PAN Peng, et al. MicroRNA- 130a inhibits spermatogenesis by directly targeting androgen receptor in mouse Sertoli cells[J]. Mol Reprod Dev, 2018, 85(10): 768-777.
[9]　　GUNES S, ARSLAN M A, HEKIM G N T, et al. The role of epigenetics in idiopathic male infertility[J]. J Assist Reprod Genet, 2016, 33(5): 553-569.
[10] CHEN Xiaoxu, CHE Dongxue, ZHANG Pengfei, et al. Profiling of miRNAs in porcine germ cells during spermatogenesis[J]. Reproduction, 2017, 154(6): 789- 798.
[11] KOTAJA N. MicroRNAs and spermatogenesis[J]. Fertil Steril, 2014, 101(6): 1552-1562.
[12] NOVESKI P, POPOVSKA-JANKOVIC K , KUBELKA-SABIT K, et al. MicroRNA expression profiles in testicular biopsies of patients with impaired spermatogenesis[J]. Andrology, 2016, 4(6): 1020-1027.
[13] WANG Cheng, YANG Cuihua, CHEN Xi, et al. Altered profile of seminal plasma microRNAs in the molecular diagnosis of male infertility[J]. Clin Chem, 2011, 57(12): 1722-1731.
[14] ABU-HALIMA M, HAMMADEH M, BACKES C, et al. Panel of five microRNAs as potential biomarkers for the diagnosis and assessment of male infertility[J]. Fertil Steril, 2014, 102(4): 989-997.

相似文献/References:

[1]李　颖,任炳臣,韩晓庆,等.支气管哮喘患者血清MicroRNA-145水平表达与肺功能、气道重塑及Th1/Th2平衡的关系分析[J].现代检验医学杂志,2021,36(05):133.[doi:10.3969/j.issn.1671-7414.2021.05.030]
　LI Ying,REN Bing-chen,HAN Xiao-qing,et al.Relationship between Serum MicroRNA-145 Expression with Lung Function,Airway Remodeling and Th1/Th2 Balance in Patients with Bronchial Asthma[J].Journal of Modern Laboratory Medicine,2021,36(03):133.[doi:10.3969/j.issn.1671-7414.2021.05.030]
[2]韩 韶,侯艳苹,马岩朋.未破裂颅内动脉瘤患者血清miR-135b 和miR-145 表达水平及临床价值研究[J].现代检验医学杂志,2023,38(04):116.[doi:10.3969/j.issn.1671-7414.2023.04.021]
　HAN Shao,HOU Yanping,MA Yanpeng.Study of Expression Levels and Clinical Value of Serum miR-135b and miR- 145 in Patients with Unruptured Intracranial Aneurysms[J].Journal of Modern Laboratory Medicine,2023,38(03):116.[doi:10.3969/j.issn.1671-7414.2023.04.021]

备注/Memo

备注/Memo:
收稿日期：2019-12-11 修回日期：2020-01-16作者简介：林彤（1978-），女，博士，主治医师，研究方向：生殖医学研究，E-mail:lt1016556985@163.com。
更新日期/Last Update: 2020-07-20