[1]陈　博,朱　娜,徐翠香,等.脑出血术后脑脊液中食管癌相关基因4和S100B蛋白表达水平与GCS评分及Barthel 指数的相关性研究[J].现代检验医学杂志,2021,36(02):11-14.[doi:doi:10.3969/j.issn.1671-7414.2021.02.003]
　CHEN Bo,ZHU Na,XU Cui-xiang,et al.Expression Level of Esophageal Cancer-Related Gene 4 and S100B Protein in Cerebrospinal Fluid after Intracerebral Hemorrhage and Its Correlation with GCS Score and Barthel Index[J].Journal of Modern Laboratory Medicine,2021,36(02):11-14.[doi:doi:10.3969/j.issn.1671-7414.2021.02.003]
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脑出血术后脑脊液中食管癌相关基因4和S100B蛋白表达水平与GCS评分及Barthel 指数的相关性研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第36卷
期数:
2021年02期
页码:
11-14
栏目:
论　著
出版日期:
2021-04-30

文章信息/Info

Title:
Expression Level of Esophageal Cancer-Related Gene 4 and S100B Protein in Cerebrospinal Fluid after Intracerebral Hemorrhage and Its Correlation with GCS Score and Barthel Index
文章编号:
1671-7414（2021）02-011-04
作者:
陈　博; 朱　娜; 徐翠香; 等
(陕西省人民医院 a.神经外科；b.科研处；c.中心实验室 陕西省感染与免疫重点实验室；d.医务处，西安 710068)
Author(s):
CHEN Bo;  ZHU Na;  XU Cui-xiang; et al
( a. Department of Neurosurgery; b. Scientific Research Division Department; c. Central Laboratory, Shaanxi Provincial Key Laboratory of Infection and Immune Diseases; d.Department of Medical Affairs, Shaanxi Provincial People’s Hospital, Xi’an 710068, China)
关键词:
脑出血; 食管癌相关基因4; S100钙结合蛋白B; 脑脊液
分类号:
R651.11；R392.11
DOI:
doi:10.3969/j.issn.1671-7414.2021.02.003
文献标志码:
A
摘要:
目的　探讨脑出血（intracerebral hemorrhage，ICH）患者术后脑脊液（CSF）中食管癌相关基因4（ECRG4）与S100钙结合蛋白B（S100B）表达水平与格拉斯哥昏迷评分（Glasgow coma scale，GCS）和巴氏指数（barthel index，BI）的相关性，及其在脑出血患者术后神经功能康复中的临床应用价值。方法　选择2017年1月~2019年12月入住陕西省人民医院神经外科治疗的基底节区ICH患者30例，采用酶联免疫吸附法（ELISA）检测10例对照组、10例腰大池引流组和10例腰椎穿刺组患者ICH术后ECRG4与S100B蛋白水平，进行GCS和BI评分，并进行相关性分析。结果　术后7天时，对照组ECRG4（1.14±0.39pg/ml）低于引流组（15.50±0.28 pg/ml）和穿刺组（10.71±0.89pg/ml），对照组 S100B（0.550±0.310ng/ml）水平高于引流组（0.154±0.025ng/ml）与穿刺组（0.192±0.030ng/ml），引流组两者表达水平与穿刺组比较，差异均有统计学意义（t=11.630，28.120，均P＜0.05）。术后7天时，引流组与穿刺组 GCS（11.62±1.71，10.19±1.41）及BI（43.24±10.05，40.64±10.10）评分均高于对照组（GCS：7.07±1.43，BI：21.73±6.45），差异具有统计学意义（t=7.466，4.180，P<0.05）。结论　ECRG4与S100B蛋白在脑出血患者脑脊液中的变化趋势与GCS和BI评分具有相关性，两者联合检测，有助于调整治疗方案，从而降低患者神经功能障碍的发生，改善预后。

参考文献/References:

[1] 《脑出血后脑水肿管理专家共识》专家组.脑出血后脑水肿管理专家共识[J].实用心脑肺血管病杂志,2017，25(8): 1-6． Expert Group of Expert Consensus on Management for Gerebral Edema Gaused by Intracerebral Hemorrhage. Expert consensus on management for cerebral edema caused by intracerebral hemorrhage [J]. Practical Journal of Cardiac Cerebral Pneumal and Vascular Disease, 2017,25(8): 1-6.
[2] 朱娜，陈博，张小艳,等.血清S100B蛋白对闭合性颅脑损伤患者诊断及预后临床价值的研究[J].现代检验医学杂志,2017,32(5):59-61. ZHU Na, CHEN Bo, ZHANG Xiaoyan, et al. Clinical value of serum S100 beta protein in the diagnosis and prognosis of patients with closed craniocerebral injury [J]. Journal of Modern Laboratory Medicine, 2017, 32 (5): 59-61.
[3] 陈斌,成宜军,陈正鸿,等.脑出血后神经细胞死亡机制的研究进展[J].中国脑血管病杂志,2018,15(3)：153-156. CHEN Bin, CHENG Yijun, CHEN Zhenghong, et al. Research progress of neural cell death after intracerebral hemorrhage [J]. Chinese Journal of Cerebrovascular Disease, 2018, 15 (3): 153-156
[4] DORSCHNER R A,LEE J, COHEN O, et al. ECRG4 regulates neutrophil recruitment and CD44 expression during the inflammatory response to injury[J]. Science Advances.2020, 6(11): eaay 0518.
[5] 王丽丽,张宁,赵迎春,等.老年急性脑出血并发癫痫患者血清HP,SOD,MDA水平表达及其与认知功能损害的相关性研究[J].现代检验医学杂志,2020,35(2):108-111. WANG Lili,ZHANG Ning,ZHAO Yingchun, et al.Study on the correlation between serum haptoglobin, superoxide dismutase and malondialdehyde in elderly patients with epilepsy after acute cerebral hemorrhage and their correlation with cognitive impairment [J].Journal of Modern Laboratory Medicine,2020,35(2): 108-111.
[6] 苏涛,刘海玲,陆士新,等.人食管癌相关集团cDNA片段的克隆与初步鉴定[J].中华肿瘤杂志，1998，20(4):254-257. SU Tao , LIU Hailing, LU Shixin, et al. Cloning and identification of cDNA fragments related to human esophageal cancer[J]. Chin J Oncol, 1998, 20(4):254-257.
[7] QIN Xin, ZHANG Ping. ECRG4: a new potential target in precision medicine[J]. Frontiers of Medicine, 2019, 13(5): 540-546.
[8] LIANG Xin, GAO Jiangang,WANG Quan, et al. ECRG4 represses cell proliferation and invasiveness via NFIC/OGN/NF-κB signaling pathway in bladder cancer[J]. Frontiers In Genetics, 2020, 11: 846.
[9] Allen Spinal Cord Atlas [Internet].Seattle(WA): Allen Institute for Brain Science[Z].2009. http//mousespinal. brain-map.arg.
[10] MAGDALENO S, JENSEN P, BRUMWELL C L, et al. BGEM: an in situ hybridization database of gene expression in the embryonic and adult mouse nervous system[J]. PLoS Biology, 2006,4(4): e86.
[11] GONZALEZ A M, PODVIN S, LIN S Y, et al.Ecrg4 expression and its product augurin in the choroid plexus: impact on fetal brain development, cerebrospinal fluid homeostasis and neuroprogenitor cell response to CNS injury[J]. Fluids and Barriers of CNS, 2011,8(1): 1-17.DOI:10.1186/2045-8118-8-6.
[12] 陈博，缪星宇，师蔚.脑血疏对脑出血大鼠脉络丛上皮细胞中食管癌相关基因4表达的影响[J].中国医药,2016,11(11):1713-1717. CHEN Bo, MIAO Xingyu, SHI Wei. Effect of naoxueshu on the expression of esophageal cancer related gene 4 in choroid plexus epithelial cells in intracerebral hemorrhagerats[J]. Chinese Medicine, 2016, 11 (11): 1713-1717.
[13] 周海航,张李涛,沈建国,等.重型颅脑损伤患者血清 S100B,IL- 6与颅内压力变化的相关性研究[J].中华全科医学，2017，15(4): 574-576. ZHOU Haihang, ZHANG Litao, SHEN Jianguo, et al. Correlation between serum S100B and IL-6 levels and intracranial pressure in patients with severe craniocerebral injury [J]. Chinese Journal of General Medicine, 2017, 15 (4): 574-576
[14] FERRETE-ARAUJO A M, RODRíGUEZ-RODRíGUEZ A, EGEA-GU ERRERO J J, et al. Brain injury biomarker behavior in spontaneous intracerebral hemorrhage [J].World Neurosurgery, 2019, 132: e496-e505.
[15] DELGADO P, ALVAREZ SABIN J, SANTAMARINA E, et al. Plasma S100B level after acute spontaneous intracerebral hemorrhage [J]. Stroke, 2006, 37(11): 2837-2839.
[16] 邱治春,王恩任.早期高压氧治疗对中重型颅脑损伤患者临床预后及血清NSE, S100B的影响 [J]. 临床外科杂志，2018，26(7): 504-506. QIU Zhichun, WANG Enren. Effect of early hyperbaric oxygen therapy on the clinical prognosis of patients with moderate to severe brain injutry and their serum NSE, S100B[J]. Journal of Clinical Surgery, 2018, 26 (7): 504-506.
[17] FERREIRA A.Diagnostic value of creat ine kinase activityin canine cerebrospinal fluid [J].Canadian Veterinary Journal,2016,57(10):1081-1086.
[18] 刘辰庚,梁巍,王培昌.成年人脑脊液CK, ACT, LDH和GPx活性检测对脑血管病的鉴别诊断价值[J]. 现代检验医学杂志,2018，33（5）：4-7. LIU Chengeng,LIANG Wei,WANG Peichang. Differential diagnostic value of CK,AST,LDH and GPx activity in adults cerebrospinal fluid of cerebrovascular diseases [J].Journal of Modern Laboratory Medicine,2018,33(5):4-7.
[19] DEMIRCI T, AYDIN M D, CAGLAR O, et al.First definition of burned choroid plexus in acidic cerebrospinal fluid‐filled brain ventricles during subarachnoid hemorrhage: Experimental study[J].Neuropathology,2020,40(3):251-260.

备注/Memo

备注/Memo:
基金项目：国家自然科学基金 (81271341)，陕西省卫计委联合攻关项目 [2016B004]。
作者简介：陈博（1979-），男，博士，副主任医师，主要研究方向为中枢神经系统损伤，E-mail：13213424@qq.com。
通讯作者：汪玲果（1971-），女，学士，主管护师，主要研究方向为神经损伤与康复, E-mail：119275360@qq.com。
更新日期/Last Update: 2021-04-10