[1]王利民,刘 锴.TUBA1C促进人肺腺癌细胞系增殖和迁移的机制研究[J].现代检验医学杂志,2021,36(04):74-80.[doi:10.3969/j.issn.1671-7414.2021.04.016]
 WANG Li-min,LIU Kai.Study on the Mechanism of TUBA1C Promoting Proliferation and Migrationof Lung Adenocarcinoma Cells[J].Journal of Modern Laboratory Medicine,2021,36(04):74-80.[doi:10.3969/j.issn.1671-7414.2021.04.016]
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TUBA1C促进人肺腺癌细胞系增殖和迁移的机制研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第36卷
期数:
2021年04期
页码:
74-80
栏目:
论 著
出版日期:
2021-07-31

文章信息/Info

Title:
Study on the Mechanism of TUBA1C Promoting Proliferation and Migrationof Lung Adenocarcinoma Cells
文章编号:
1671-7414(2021)04-074-07
作者:
王利民刘 锴
(河北省中医院肿瘤科 石家庄 050011)
Author(s):
WANG Li-minLIU Kai
(Department of Oncology, Hebei Provincial Hospital of Traditional, Shijiazhuang 050011, China)
关键词:
肺腺癌TUBA1CE2F1MYC.
分类号:
R734.2;R730.43
DOI:
10.3969/j.issn.1671-7414.2021.04.016
文献标志码:
A
摘要:
目的 探究TUBA1C 在肺腺癌中的表达特征及其对人肺腺癌细胞系增殖、迁移的影响和相关潜在分子机制。方法 通过临床数据库GEPIA 检索TUBA1C 在肺腺癌中的表达特征以及与临床预后相关性;通过30 组临床组织样本探究TUBA1C 在肺腺癌及其临近正常组织中的表达特征;通过siRNA 介导敲低TUBA1C 表达,利用细胞增殖实验、克隆形成实验、划痕迁移实验和细胞周期实验分别检测TUBA1C 对肺腺癌细胞生物学行为的影响;通过分析与TUBA1C 共表达基因参与的信号通路,探索TUBA1C 在肺腺癌发展进程中的潜在分子机制,并进一步通过回补实验进行验证。结果 GEPIA 数据库检索显示肺腺癌中TUBA1C 显著高表达(P<0.05),且高表达的病人具有不良预后(logrankP=9.3E-5)。30 组肺腺癌临床样本中TUBA1C 表达水平(6.4±1.3)显著高于癌旁正常组织(5.2±0.9),差异有统计学意义(t=4.157,P < 0.001),且随着癌症的不断恶化,TUBA1C 表达逐渐升高(P=0.003 49)。转染培养3,4 和5天时siTUBA1CA#1 组和siTUBA1CA#2 组细胞增殖A 值明显低于对照组,差异均有统计学意义(F=7.000~27.780,P< 0.05)。siTUBA1CA#1 组(41.2±1.5)和siTUBA1CA#2 组(40.3±1.3)细胞克隆形成率明显低于对照组(72.4±2.2),差异有统计学意义(F=342.482,P < 0.001);两实验组细胞迁移率为73.4±3.2 和72.1±2.8,明显低于对照组(98.6±1.7),差异有统计学意义(F=95.778,P < 0.001);细胞周期较对照组相比显著阻滞在G1 期。siTUBA1CA#1 组(0.21±0.02,0.33±0.03)和siTUBA1CA#2 组(0.20±0.03,0.36±0.02)细胞中E2F1 和MYC mRNA 表达水平较对照组(1.01±0.03,1.00±0.02)明显降低,差异有统计学意义(F=883.773,758.294,均P < 0.001)。在敲低TUBA1C 表达的细胞中分别回补E2F1,MYC 以及共回补E2F1 和MYC 后,细胞增殖速率、迁移速率及细胞周期较单纯敲低TUBA1C 组相比均逐渐恢复正常(P<0.01),接近正常水平。结论  肺腺癌中TUBA1C 高表达,通过激活促癌基因E2F1 和MYC 的表达促进肺腺癌细胞的增殖、克隆形成及迁移,诱导细胞凋亡,参与促进肺腺癌的发展进程。

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备注/Memo

备注/Memo:
基金项目:河北省卫生和计划生育委员会资助项目课题(编号2018021)。
作者简介:王利民(1975-),男,硕士研究生,主治医师,研究方向:肿瘤学,E-mail:gongjilu8631@126.com。
通讯作者:刘锴(1977-),男,本科,主治医师,研究方向:肿瘤微创治疗。
更新日期/Last Update: 1900-01-01