[1]沈伟兴a,汤佳瑾b,傅　鹏a.原发性IgA肾病患者血清miR-181b水平表达与病情严重程度及预后的相关性分析[J].现代检验医学杂志,2021,36(06):51-54.[doi:10.3969/j.issn.1671-7414.2021.06.010]
　SHEN Wei-xing,TANG Jia-jin,FU Peng.Analysis of the Correlation between the Expression of Serum miR-181b and the Severity and Prognosis of Patients with Primary IgA Nephropathy[J].Journal of Modern Laboratory Medicine,2021,36(06):51-54.[doi:10.3969/j.issn.1671-7414.2021.06.010]
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原发性IgA肾病患者血清miR-181b水平表达与病情严重程度及预后的相关性分析()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第36卷
期数:
2021年06期
页码:
51-54
栏目:
论　著
出版日期:
2021-12-15

文章信息/Info

Title:
Analysis of the Correlation between the Expression of Serum miR-181b and the Severity and Prognosis of Patients with Primary IgA Nephropathy
文章编号:
1671-7414（2021）06-051-05
作者:
沈伟兴a; 汤佳瑾b; 傅　鹏a
（上海市市东医院a.肾内科；b.重症医学科，上海 200438）
Author(s):
SHEN Wei-xing;  TANG Jia-jin;  FU Peng
（a. Department of Nephrology; b. Department of Critical Care Medicine, Shidong Hospital of Shanghai, Shanghai 200438, China）
关键词:
IgA肾病; 微小RNA-181b; 严重程度
分类号:
R692；R392.11
DOI:
10.3969/j.issn.1671-7414.2021.06.010
文献标志码:
A
摘要:
目的　分析原发性IgA肾病患者血清miR-181b水平表达及与病情严重程度、预后的相关性。方法　回顾性分析上海市市东医院2015年6月~2017年11月收治的原发性IgA肾病患者107例的临床资料。根据患者肾脏病理学检查结果按病情严重程度分为轻度、中度、重度，根据随访结果将预后分为预后良好和预后较差。比较不同病情严重程度和不同预后患者的血清miR-181b水平。采用Spearman相关分析miR-181b水平与IgA肾病病情严重程度及预后的相关性，绘制ROC曲线分析血清miR-181b水平对IgA肾病疾病严重程度及预后的预测价值。结果　107例原发性IgA肾病患者中轻度35例、中度46例、重度26例。轻度、中度和重度患者的血清miR-181b水平分别为(1.47±0.41)×2-△△Ct，(1.89±0.58)×2-△△Ct和(2.69±0.67)×2-△△Ct，差异有统计学意义(F=49.765，P<0.001)。107例原发性IgA肾病患者随访2年后，预后良好88例、预后较差19例。预后良好、预后较差患者的血清miR-181b水平分别为(1.73±0.52)×2-△△Ct和(2.95±0.74)×2-△△Ct，差异有统计学意义(t=5.193，P<0.001)。经Spearman相关分析发现，血清miR-181b水平与患者病情严重程度(r=0.801，P<0.001)、预后(r=0.494，P<0.001)呈显著相关性。血清miR-181b预测IgA肾病病情严重程度的ROC曲线下面积为0.886(95%CI：0.717~0.966)，最佳临界值2.25×2-△△Ct，此时灵敏度和特异度分别为0.892，0.731。血清miR-181b预测IgA肾病预后的ROC曲线下面积为0.848(95%CI：0.701~0.925)，最佳临界值2.48×2-△△Ct，此时灵敏度和特异度分别为0.873和0.704。结论　血清miR-181b水平与IgA肾病病情严重程度及预后均呈显著相关性，血清miR-181b越高，病情越重，预后越差。可通过检测IgA肾病患者血清miR-181b水平评估患者病情及预测预后。
Abstract:
Objective　To analyze the expression of serum miR-181b level in patients with primary IgA nephropathy and its correlation with the severity and prognosis of the disease. Methods　The clinical data of 107 patients with primary IgA nephropathy admitted to Shanghai Shidong Hospital from June 2015 to November 2017 were retrospectively analyzed. According to the renal pathological examination results of the patients, the severity of the disease was divided into mild, moderate and severe. According to the follow-up results, the prognosis was divided into good prognosis and poor prognosis. The serum miR-181b levels of patients with different disease severity and different prognosis were compared. Spearman correlation analysis was used to analyze the correlation between miR-181b level and the severity and prognosis of IgA nephropathy. ROC curve was drawn to analyze the predictive value of serum miR-181b level on the severity and prognosis of IgA nephropathy. Results　Among 107 patients with primary IgA nephropathy, 35 were mild, 46 were moderate, and 26 were severe. Serum miR-181b levels in mild, moderate and severe patients were (1.47±0.41) ×2-△△Ct, (1.89±0.58) ×2-△△Ct and (2.69±0.67)×2-△△Ct, and the difference was statistically significant(F=49.765, P<0.001). After 107 patients with primary IgA nephropathy were followed up for 2 years, 88 had a good prognosis and 19 had a poor prognosis. Serum miR-181b levels of patients with good prognosis and poor prognosis were (1.73±0.52)×2-△△Ct and (2.95±0.74)×2-△△Ct, the difference was statistically significant (t=5.193，P<0.001). According to Spearman correlation analysis, there was a significant correlation between serum miR-181b level and the severity of the patient’s condition (r = 0.801, P<0.001), prognosis (r = 0.494, P<0.001). The area under the ROC curve of serum miR-181b to predict the severity of IgA nephropathy was 0.886 (95% CI: 0.717~0.966), the optimal cut-off value was 2.25×2-△△Ct, the sensitivity and the specificity were 0.892 and 0.731, respectively; The miR-181b predicts the area under the ROC curve of IgA nephropathy prognosis 0.848 (95% CI: 0.701~0.925), the optimal cut-off value was 2.48×2-△△Ct, the sensitivity and the specificity were 0.873 and 0.704. Conclusion　The level of serum miR-181b was significantly correlated with the severity and prognosis of IgA nephropathy. The higher the serum miR-181b, the more serious the disease and the wore prognosis. The condition and prognosis of patients with IgA nephropathy can be evaluated by detecting the levels of serum miR-181b.

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备注/Memo

备注/Memo:
作者简介：沈伟兴（1982-），男，本科，主治医师，研究方向：IgA肾病临床发病机制。通讯作者：傅鹏，主任医师，研究方向：重症医学。
更新日期/Last Update: 1900-01-01