[1]陈映含.糖尿病肾脏病患者血清 let-7d mRNA的相对表达量与肾功能进展的相关性研究[J].现代检验医学杂志,2022,37(01):77-81.[doi:10.3969/j.issn.1671-7414.2022.01.016]
　CHEN Ying-han.Study on the Correlation between Relative Expression of Serum let-7d mRNA and Renal Function Progression in Patients withDiabetic Nephropathy[J].Journal of Modern Laboratory Medicine,2022,37(01):77-81.[doi:10.3969/j.issn.1671-7414.2022.01.016]
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糖尿病肾脏病患者血清 let-7d mRNA的相对表达量与肾功能进展的相关性研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第37卷
期数:
2022年01期
页码:
77-81
栏目:
论　著
出版日期:
2022-01-15

文章信息/Info

Title:
Study on the Correlation between Relative Expression of Serum let-7d mRNA and Renal Function Progression in Patients withDiabetic Nephropathy
文章编号:
1671-7414（2022）01-077-05
作者:
陈映含
（赤峰市医院内分泌科，内蒙古赤峰 024000）
Author(s):
CHEN Ying-han
（Department of Endocrinology, Chifeng Hospital ,Inner Mongolia Chifeng 024000, China）
关键词:
糖尿病肾脏病; let-7d; 肾功能; 肾纤维化
分类号:
R587.2；R446.112
DOI:
10.3969/j.issn.1671-7414.2022.01.016
文献标志码:
A
摘要:
目的　探讨血清 let-7d与糖尿病肾脏病（ diabetic kidney disease, DKD）患者肾功能进展的关系。方法　选取 2017年 4月～ 2018年 10月期间在赤峰市医院治疗的 DKD患者 164例，所有患者在入组后均进行 2年的随访，共有 154例患者有完整的随访资料，根据肾功能进展情况将其分为进展组（ 52例）和未进展组（ 102例）。采用实时荧光定量 PCR技术检测 let-7d mRNA的相对表达量，分析 DKD患者的肾功能进展的影响因素，分析 DKD患者血清 let-7d mRNA相对表达量与相关指标的相关性，采用接受者工作特征曲线（ ROC）分析血清 let-7d mRNA的相对表达量对 DKD患者肾功能进展的预测价值。结果　进展组的病程长于未进展组，血肌酐（SCr）、尿素氮（BUN）、胱抑素 C（Cys C）、转化生长因子 -β1（TGF-β1）水平均高于未进展组，肾小球滤过率（eGFR）、let-7d mRNA相对表达量均低于未进展组，差异均有统计学意义（ t=2.403~7.436，均 P<0.05）。多因素非条件 Logistic回归分析显示， Cys C和 TGF-β1水平过高均是 DKD患者肾功能进展的危险因素，而 eGFR和 let-7d mRNA相对表达量水平过高则是 DKD患者肾功能进展的保护因素（ P<0.05）。Pearson分析显示， DKD患者血清 let-7d mRNA相对表达量与 SCr，BUN，Cys C和 TGF-β1均呈负相关（r=-0.491~-0.302，均 P<0.05），与 eGFR呈正相关（r=0.405，P<0.05）。ROC分析结果显示，血清 let-7d mRNA的相对表达量对 DKD患者肾功能进展的预测价值较高，曲线下面积为 0.874。结论　let-7d与 DKD患者肾功能进展情况密切相关，其可能是通过调控肾纤维化参与疾病的发生、发展。
Abstract:
Objective　To investigate the relationship between serum let-7d and the progression of renal function in patientswith diabetic kidney disease (DKD). Methods　A total of 164 patients with DKD who were treated in Chifeng Hospital fromApril 2017 to October 2018 were selected. All patients were followed up for 2 years after enrollment. A total of 154 patients hadcomplete follow-up data. According to the progress of renal function, they were divided into progressive group (52 cases) andnon progressive group (102 cases). The relative expression of let-7d mRNA was detected by real-time PCR. The influencingfactors of renal function progression in patients with DKD was analyzed. The correlation between the relative expression of let-7d mRNA in serum of patients with DKD and related indexes was analyzed. Receiver operating characteristic curve (ROC) wasused to analyze the predictive value of the relative expression of let-7d mRNA in serum for the progression of renal function inpatients with DKD. Results　The disease course of the progressive group was longer than that of the non progressive group,serum creatinine (SCr), urea nitrogen (BUN), cystatin C (Cys C) and transforming growth factor-β1 (TGF-β1) levels werehigher than that of the non progressive group, the estimated Glomerular filtration rate (eGFR) and the relative expression of let-7d mRNA were lower than those of the non progressive group, and the differences were statistically significant (t=2.403~7.436,all P<0.05). Multivariate unconditional Logistic regression analysis showed that excessively high levels of Cys C and TGF-β1were risk factors for the progression of renal function in patients with DKD, while excessively high levels of eGFR and let-7dmRNA were protective factors for the progression of renal function in patients with DKD (P<0.05). Pearson analysis showedthat the relative expression of let-7d mRNA in serum of patients with DKD was negatively correlated with SCr, BUN, Cys C andTGF-β1 (r =-0.491~-0.302, all P<0.05), and it was positively correlated with eGFR (r=0.405,P<0.05). ROC analysis resultsshowed that the relative expression of serum let-7d mRNA had a higher predictive value for the progression of renal function inpatients with DKD, and the area under the curve was 0.874. Conclusion　Let-7d was closely related to the progression of renalfunction in patients with DKD. It may be involved in the occurrence and development of the disease by regulating renal fibrosis.

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备注/Memo

备注/Memo:
作者简介：陈映含（1989-），女，硕士，主治医师，研究方向：临床内分泌相关疾病的诊治，E-mail：cyh763998@163.com。
更新日期/Last Update: 1900-01-01