[1]李　娜,黄友军,李志超,等.慢性咳嗽变异性哮喘患者外周血单个核细胞中STAT1，IRF1蛋白水平与临床急性发作的相关性分析[J].现代检验医学杂志,2022,37(02):110-114.[doi:10.3969/j.issn.1671-7414.2022.02.023]
　LI Na,HUANG You-jun,LI Zhi-chao,et al.Analysis on Correlation between STAT1, IRF1 Protein Levels in Peripheral Blood Mononuclear Cells and Clinical Acute Attack in Patients with Chronic Cough Variant Asthma[J].Journal of Modern Laboratory Medicine,2022,37(02):110-114.[doi:10.3969/j.issn.1671-7414.2022.02.023]
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慢性咳嗽变异性哮喘患者外周血单个核细胞中STAT1，IRF1蛋白水平与临床急性发作的相关性分析()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第37卷
期数:
2022年02期
页码:
110-114
栏目:
论　著
出版日期:
2022-03-15

文章信息/Info

Title:
Analysis on Correlation between STAT1, IRF1 Protein Levels in Peripheral Blood Mononuclear Cells and Clinical Acute Attack in Patients with Chronic Cough Variant Asthma
文章编号:
1671-7414（2022）02-110-05
作者:
李　娜;  黄友军; 李志超; 魏旺丽; 张　林
（郴州市第一人民医院检验科，湖南郴州 423000）
Author(s):
LI Na;  HUANG You-jun;  LI Zhi-chao;  WEI Wang-li;  ZHANG Lin
（Department of Climical Laboratory Medicine, the First People’s Hospital of Chenzhou , Hunan Chenzhou 423000, China）
关键词:
慢性咳嗽变异性哮喘; 信号转导子和转录激活子-1; 干扰素调节因子-1
分类号:
R562.25；R446.11
DOI:
10.3969/j.issn.1671-7414.2022.02.023
文献标志码:
A
摘要:
目的　探讨慢性咳嗽变异性哮喘患者外周血单个核细胞信号转导子和转录激活子-1（signal transducer and activator of transcription-1，STAT1）和干扰素调节因子-1（interferon regulatory factor 1，IRF1）蛋白水平与临床急性发作的相关性。方法　收集2018 年8 月～ 2020 年3 月郴州市第一人民医院收治的52 例慢性咳嗽变异性哮喘急性发作患者纳入观察组。同期慢性咳嗽变异性哮喘非急性发作患者52 例纳入对照组。采用蛋白质免疫印迹（western Blot,WB）法检测STAT1 和IRF1 蛋白表达，分析其与慢性咳嗽变异性哮喘急性发作的关系。结果　观察组STAT1（1.28±0.37），IRF1（1.17±0.35）均高于对照组（1.05±0.27， 0.81±0.25），差异有统计学意义（t=3.321，6.036，均P ＜ 0.05）；经双变量Pearson 直线相关检验，慢性咳嗽变异性哮喘患者STAT1 与IRF1 蛋白表达呈正相关（r=0.322，P ＜ 0.001）；经Logistic 回归分析，STAT1 和IRF1 蛋白过表达可能是诱发慢性咳嗽变异性哮喘急性发作的影响因素（OR ＞ 1，P＜ 0.001）；绘制ROC曲线发现，STAT1，IRF1 蛋白表达及两指标联合评估慢性咳嗽变异性哮喘急性发作的AUC均＞ 0.85,具有一定预测价值，其中联合检测预测价值最高。结论　慢性咳嗽变异性哮喘急性发作患者STAT1 和IRF1 呈高表达，其水平越高则患者急性发作风险越高，可作为以急性发作预测的有效指标。
Abstract:
Objective　To investigate the correlation between the levels of signal transducer and activator of transcription-1 (STAT1), interferon regulatory factor-1 (IRF1) protein in peripheral blood mononuclear cells and clinical acute attacks in patients with chronic cough variant asthma. Methods　52 patients with acute attacks of chronic cough variant asthma treated in the First People’s Hospital of Chenzhou from August 2018 to March 2020 were collected and included in the observation group. 52 patients with non-acute attacks of chronic cough variant asthma during the same period were included in the control group. Western blot (WB) was used to detect the expression of STAT1 and IRF1 protein, and the relationship between them and acute attacks of chronic cough variant asthma was analyzed. Results　STAT1 (1.28±0.37), IRF1 (1.17±0.35) of the observation group were higher than the control group(1.05±0.27, 0.81±0.25) , the differences were statistically significant (t=3.321, 6.036, all P<0.05) . The bivariate Pearson linear correlation test showed that the expression of STAT1 and IRF1 protein of patients with chronic cough variant asthma was positively correlated (r=0.322, P<0.001) . The results of Logistic regression analysis showed that the overexpression of STAT1 and IRF1 protein might be the influencing factor for the acute attacks of chronic cough variant asthma (OR>1, P<0.001) .The ROC curve was drawn and found that the AUC of STAT1, IRF1 protein expression and the combination of the two indexes in predicting the acute attacks of chronic cough variant asthma were 0.861, 0.854, both>0.85, which had certain predictive value, of which the combined predictive value was the highest. Conclusion　STAT1 and IRF1 in patients with acute attacks of chronic cough variant asthma are overexpressed, the higher the level of them, the higher the risk of acute attacks in patients, which can be used as an effective index to predict acute attack.

参考文献/References:

[1] 龚琳婧, 佘君, 朱蕾. 白三烯在咳嗽变异性哮喘发病机制中的作用[J]. 中华结核和呼吸杂志, 2019, 42(3):219-222. GONG Linjing, SHE Jun, ZHU Lei. The role of leukotriene in the pathogenesis of cough variant asthma [J]. Chinese Journal of Tuberculosis and Respiratory Diseases, 2019, 42(3): 219-222.
[2] 丁珊, 方声, 叶秀霞. 呼吸道病毒感染对哮喘患儿气 道重塑与炎症反应的影响[J]. 中华医院感染学杂志, 2019, 29(4):625-627, 640. DING Shan, FANG Sheng, YE Xiuxia. Effect of respiratory virus infection on airway remodeling and inflammatory response in children with asthma [J]. Chinese Journal of Nosocomiology, 2019, 29 (4): 625-627, 640.
[3] 王萍, 张熊．血清β2-AR mRNA 和NGF mRNA 水 平检测在儿童支气管哮喘气道重塑中的应用价值 [J]. 现代检验医学杂志, 2017, 32(5):69-73. WANG Ping, ZHANG Xiong. Clinic application of levels of serum β2-AR mRNA and NGF mRNA for children with the bronchial asthma in airway remodeling[J]. Journal of Modern Laboratory Medicine, 2017, 32(5): 69-73.
[4] KOHANBASH G, CARRERA D A, SHRIVASTAV S, et al.Isocitrate dehydrogenase mutations suppress STAT1 and CD8+ T cell accumulation in gliomas [J]. The Journal of Clinical Investigation, 2017, 127(4): 1425-1437.
[5] CHEN Zhihong, SU Xiaoqiong, PAN Jue, et al. Preventative administration of IL-27 improves airway inflammation of ovalbumin - induced asthma mice model via both STAT1 and GADD45 gamma/p38MAPK pathways[J]. European Respiratory Journal, 2016, 48 (s60):PA1097.
[6] 陈凯鹏, 林惠玲, 张超, 等. 信号转导子和转录激活 子-1、干扰素调节因子-1 及干扰素-γ 在支气管哮 喘儿童中的表达及其临床意义[J]. 中国小儿急救医 学, 2018, 25(11):834-838. CHEN Kaipeng, LIN Huiling, ZHANG Chao, et al. Expression and clinical significance of signal transducer and activator of transcription-1, interferon regulatory factor-1,and interferon-γ in children with bronchial asthma [J]. Chinese Pediatric Emergency Medicine, 2018, 25(11): 834-838.
[7] DENG Songyun, ZHANG Lemeng, AI Yuhang, et al.Role of interferon regulatory factor-1 in lipopolysaccharide-induced mitochondrial damage and oxidative stress responses in macrophages[J]. Int J Mol Med, 40(4):1261-1269.
[8] 中华医学会呼吸病学分会哮喘学组．咳嗽的诊断 与治疗指南(2015)[J]．中华结核和呼吸杂志, 2016, 39(5):323-354. Asthma Group of Chinese Throacic Society of Chinese Medical Association. Guidelines for diagnosis and treatment of cough (2015) [J]. Chinese Journal of Tuberculosis and Respiratory Diseases, 2016, 39 (5): 323-354.
[9] 赵淑萍. 穴位敷贴联合宣肺平嗽汤治疗小儿咳嗽变 异性哮喘风邪犯肺证的效果及对患者炎症因子、免 疫功能的影响[J]. 陕西中医, 2019, 40(7): 954-956. ZHAO Shuping. The effect of acupoint application combined with xuanfeipingsoutang on the syndrome of lung disease caused by wind pathogenic factors of cough variant asthma in children and its influence on inflammatory factors and immune function of patients[J]. Shaanxi Journal of Traditional Chinese Medicine, 2019, 40(7): 954-956.
[10] 王晟, 陈乐. 咳嗽变异性哮喘患儿布地奈德吸入剂 与孟鲁斯特钠联合治疗对血清IL-6,TNF-α,TGF-β 和IgE 水平影响及作用机制研究[J]. 现代检验医学 杂志, 2019, 34(1):137-141. WANG Sheng, CHEN Le. Effects of budesonide inhalation combined with montelukast sodium on serum IL-6,TNF-α,TGF-β1 and IgE levels in children with cough variant asthma and its mechanism [J]. Journal of Modern Laboratory Medicine, 2019, 34(1): 137-141.
[11] 孙婧怡, 马礼兵. 咳嗽变异性哮喘的发病机制及研 究进展[J]. 实用医学杂志, 2018, 34(16):2803-2806. SUN Jingyi, MA Libing. Pathogenesis and research progress of cough variant asthma[J]. The Journal of Practical Medicine, 2018, 34(16): 2803-2806.
[12] BALLEGEER M, VAN LOOVEREN K, TIMMERMANS S, et al. Glucocorticoid receptor dimers control intestinal STAT1 and TNF-induced inflammation in mice[J]. the Journal of Clinical Investigation, 2018, 128(8):3265-3279.
[13] LI Xiaoquan, LI Xiaoning, LIANG Jingjing, et al. IRF1 up-regulates isg15 gene expression in dsRNA stimulation or CSFV infection by targeting nucleotides -487 to -325 in the 5’ flanking region [J].Mol Immunol, 2018, 94:153-165.
[14] PIRAULT J, POLYZOS K A, PETRI M H, et al. The inflammatory cytokine interferon-gamma inhibits sortilin-1 expression in hepatocytes via the JAK/STAT pathway[J]. European Journal of Immunology, 2017, 47(11): 1918-1924.
[15] 徐瑞敏, 陈志康, 甘惠玲, 等. 过表达STAT1 与 CD74CD44促进结肠癌细胞粘附和迁移的相关性[J]. 实用癌症杂志, 2017, 32(7):1094-1098. XU Ruimin, CHEN Zhikang, GAN Huiling, et al. Overexpression of STAT1 and CD74CD44 to promote adhesion and migration of colon cancer cells[J]. J Pract Canc, 2017, 32(7): 1094-1098.
[16] 王玲, 黄佳．五味子乙素调控JAK2/STAT3 信号 通路抑制心肌细胞氧化应激损伤[J]. 西部中医药, 2020, 33(2):11-14. WANG Ling, HUANG Jia. Schisandrin B regulating JAK2/STAT3 signaling pathway in the inhibition of oxidative stress damage of myocardial cells [J]. West Chin Med, 2020, 33(2): 11-14.
[17] KARWACZ K, MIRALDI E R, POKROVSKII M, et al. Critical role of IRF1 and BATF in forming chromatin landscape during type 1 regulatory cell differentiation[J]. Nature Immunology, 2017, 18(4): 412-421.
[18] RUAN Baiye, CHEN Shannan, HOU Jing, et al. Two type II IFN members, IFN-γ and IFN-γ related (rel), regulate differentially IRF1 and IRF11 in zebrafish[J]. Fish Shellfish Immunol, 2017, 65:103-110.
[19] 曹朝晖, 胡聪, 吴颛, 等．LIGHT 协同IFN-γ 通过 激活STAT1 诱导MIN6 细胞凋亡[J]. 免疫学杂志, 2018, 34(10):843-849. CAO Zhaohui, HU Cong, WU Zhuan, et al. The combination of LIGHT and IFN-γ induces MIN6 cells apoptosis via activating STAT1[J]. Immun J, 2018, 34(10): 843-849.

备注/Memo

备注/Memo:
作者简介：李娜 （1986-），女，硕士，主管检验师，研究方向：检验医学。E-mail:chengshiys023@sina.com。
通讯作者：张林（1985-），女，硕士，主管检验师，研究方向：基础医学。
更新日期/Last Update: 1900-01-01