[1]张 璇,赵爱琴,邹 丹,等.子宫内膜异位症患者血清miR-455 和FABP4 表达水平及临床意义[J].现代检验医学杂志,2022,37(04):49-52+158.[doi:10.3969/j.issn.1671-7414.2022.04.010]
　ZHANG Xuan,ZHAO Ai-qin,ZOU Dan,et al.Expression Level and Clinical Significance of Serum miR-455 and FABP4 in Patients with Endometriosis[J].Journal of Modern Laboratory Medicine,2022,37(04):49-52+158.[doi:10.3969/j.issn.1671-7414.2022.04.010]
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子宫内膜异位症患者血清miR-455 和FABP4 表达水平及临床意义()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第37卷
期数:
2022年04期
页码:
49-52+158
栏目:
论著
出版日期:
2022-07-15

文章信息/Info

Title:
Expression Level and Clinical Significance of Serum miR-455 and FABP4 in Patients with Endometriosis
文章编号:
1671-7414（2022）04-049-05
作者:
张 璇; 赵爱琴; 邹 丹; 陆海英; 周素芳; 王晓庆
苏州高新区人民医院妇产科，江苏苏州 215000
Author(s):
ZHANG Xuan;  ZHAO Ai-qin;  ZOU Dan;  LU Hai-ying;  ZHOU Su-fang;  WANG Xiao-qing
Department of Obstetrics and Gynecology, People’s Hospital of Suzhou High-tech Zone, Jiangsu Suzhou 215000, China
关键词:
微小RNA-455; 脂肪酸结合蛋白4; 子宫内膜异位症
分类号:
R711.32；R392.11
DOI:
10.3969/j.issn.1671-7414.2022.04.010
文献标志码:
A
摘要:
目的 检测子宫内膜异位症（endometriosis，EMT）患者血清微小核糖核酸（miRNA, miR）-455 和脂肪酸结合蛋白4（fatty acid binding protein 4，FABP4）水平，并探讨二者的相关性及临床意义。方法 选择2018 年6 月～ 2021年3 月苏州高新区人民医院妇产科收治的73 例EMT 患者作为观察对象（EMT 组），另选取同期健康体检人员75 例作为对照组，采用实时荧光定量PCR（qRT-PCR）检测两组血清miR-455 水平，ELISA 法检测血清FABP4 水平，Pearson法分析EMT 患者血清miR-455 和FABP4 表达水平相关性，ROC 曲线分析血清miR-455，FABP4 表达对EMT 的诊断价值。结果 与对照组相比，EMT 组患者血清FABP4 水平(17.34±4.25ng/ml vs 8.56±2.74ng/ml) 显著增高，miR-455 水平显著降低（0.67 ±0.08 vs 1.05± 0.19），差异均有统计学意义（t=14.977，15.780，均P ＜ 0.001）。随着EMT 分期的升高，EMT 患者血清FABP4 表达水平显著升高，血清miR-455 表达水平显著降低，差异均有统计学意义（F=31.108，160.947，均P ＜ 0.001）。相关性分析显示，EMT 患者血清miR-455 表达水平与FABP4 呈负相关（r= -0.482，P ＜ 0.05）。血清miR-455 和FABP4 联合诊断EMT 的曲线下面积为0.922，敏感度和特异度分别为87.67% 和81.33%。结论 EMT患者血清中miR-455 低表达，FABP4 水平较高，与患者病情严重程度相关，可能成为EMT 早期诊断的分子标志物。
Abstract:
Objective To detect the levels of serum microRNA-455 (miR-455) and fatty acid binding protein 4 (FABP4) in patients with endometriosis (EMT), and explore the correlation between the two and clinical significance. Methods 73 EMT patients admitted to Department of Obstetrics and Gynecology, People’s Hospital of Suzhou High-tech Zone from June 2018 to March 2021 were selected as the observation objects (EMT group), and 75 healthy physical examinee during the same period were selected as the control group. Real-time fluorescent quantitative PCR (qRT-PCR) was used to detect the level of serum miR- 455 of subjects in the two groups, ELISA method was used to detect the levels of serum FABP4 , Pearson method was used to analyze the correlation between miR-455 and FABP4 in EMT patients, ROC curve was used to analyze the diagnostic value of serum miR-455 and FABP4 expression for EMT. Results Compared with the control group, the serum FABP4 (17.34±4.25ng/ ml vs 8.56±2.74ng/ml) level of patients in the EMT group was significantly increased, and the level of miR-455（0.67±0.08 vs 1.05±0.19） was significantly decreased, the differences were statistically significant(t=14.977, 15.780, all P<0.001). With the increase of EMT staging, the expression level of serum FABP4 in EMT patients increased significantly ，and the expression level of serum miR-455 decreased significantly, the differences were statistically significan（t F =31.108，160.947, all P＜0.001）. Correlation analysis showed that serum miR-455 expression level of EMT patients was negatively correlated with FABP4 (r= -0.482, P<0.05). The area under the curve for the combined diagnosis of serum miR-455 and FABP4 for EMT was 0.922, the sensitivity and the specificity were 87.67% and 81.33%, respectively. Conclusion The expression of miR-455 was low in the serum of EMT patients and the level of FABP4 is high, they were related to the severity of the disease, and may become molecular markers for the early diagnosis of EMT.

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备注/Memo

备注/Memo:
基金项目：江苏省妇幼健康科研项目（F201668）。
作者简介：张璇（1990-），女，硕士研究生，医师，专业：中西医结合妇产科，E-mail：zhangzg819@163.com。
通讯作者：王晓庆（1980-），女，本科，学士，副主任医师，主要从事临床妇产科工作。
更新日期/Last Update: 1900-01-01