[1]姜万嵩,韩庚奋,刘 成,等.踝关节骨折后创伤性骨性关节炎患者外周血IL-1β 和MMP-13 水平及其与关节功能的相关性研究[J].现代检验医学杂志,2022,37(05):171-175.[doi:10.3969/j.issn.1671-7414.2022.05.034]
　JIANG Wan-song,HAN Geng-fen,LIU Cheng,et al.Study on the Levels of IL-1β and MMP-13 in Peripheral Blood of Patients with Traumatic Osteoarthritis after Ankle Fracture and Their Relationship with Joint Function[J].Journal of Modern Laboratory Medicine,2022,37(05):171-175.[doi:10.3969/j.issn.1671-7414.2022.05.034]
点击复制

踝关节骨折后创伤性骨性关节炎患者外周血IL-1β 和MMP-13 水平及其与关节功能的相关性研究()
分享到：

《现代检验医学杂志》[ISSN:/CN:]

卷:
第37卷
期数:
2022年05期
页码:
171-175
栏目:
检验与临床
出版日期:
2022-09-15

文章信息/Info

Title:
Study on the Levels of IL-1β and MMP-13 in Peripheral Blood of Patients with Traumatic Osteoarthritis after Ankle Fracture and Their Relationship with Joint Function
文章编号:
1671-7414（2022）05-171-05
作者:
姜万嵩; 韩庚奋; 刘 成; 张成生; 张景阳; 马 骁
解放军第九六九医院骨科，呼和浩特 010051
Author(s):
JIANG Wan-song;  HAN Geng-fen;  LIU Cheng;  ZHANG Cheng-sheng;  ZHANG Jing-yang;  MA Xiao
Department of Orthopedics, the 969th Hospital of Chinese PLA, Hohhot 010051, China
关键词:
踝关节骨折; 创伤性骨性关节炎; 白介素-1β; 基质金属蛋白酶-13; 关节功能
分类号:
R684；R392.11
DOI:
10.3969/j.issn.1671-7414.2022.05.034
文献标志码:
A
摘要:
目的　探讨踝关节骨折后创伤性骨性关节炎患者外周血白介素-1β（interleukin-1β，IL-1β）和基质金属蛋白酶-13（matrix metalloproteinase-13，MMP-13）水平变化与关节功能的相关性。方法　选取2018 年9 月～ 2020 年6月解放军第九六九医院骨科收治的93 例踝关节骨折后创伤性骨性关节炎患者作为研究组，另选取同期健康体检者79 例作为对照组。两组均采用酶联免疫吸附实验检测外周血IL-1β，MMP-13 水平及行踝关节功能测评[ 踝- 后足功能评分（AOFAS）与Kofoed 踝关节评分] 并对比，另比较研究组不同严重程度患者外周血IL-1β，MMP-13 水平及踝关节功能评分，且采用Pearson 相关性分析法分析研究组患者外周血IL-1β，MMP-13 水平与AOFAS 评分、Kofoed 踝关节评分的相关性。结果　研究组外周血IL-1β（50.36±7.82pg/ml），MMP-13 水平（2.09±0.35ng/L）与对照组（9.84±1.13pg/ml，1.16±0.22 ng/L）比较均升高，差异有统计学意义（t=45.632，20.431，均P＜ 0.05）；研究组AOFAS评分（54.69±9.23分）、Kofoed 踝关节评分（52.74±8.61 分），与对照组比较（90.25±6.27 分，89.06±7.34 分）均降低，差异均有统计学意义（t=29.019，29.480，均P ＜ 0.05）；中度、重度患者外周血IL-1β，MMP-13 水平均高于轻度患者（t=16.228，5.474；21.840，11.862，均P ＜ 0.05），重度患者均高于中度患者（t=12.300，8.146，均P ＜ 0.05），差异有统计学意义；中度、重度患者AOFAS 评分、Kofoed 踝关节评分均低于轻度患者（t=2.875，3.233；7.444，7.690，均P ＜ 0.05），重度患者均低于中度患者（t=6.242，5.840，均P ＜ 0.05），差异均有统计学意义。经Pearson 相关性分析，研究组患者外周血IL-1β，MMP-13 水平均与AOFAS 评分、Kofoed 踝关节评分呈显著负相关（r= -0.852，-0.846；-0.873，-0.868，均P＜ 0.05）。结论　踝关节骨折后创伤性骨性关节炎患者外周血IL-1β，MMP-13 水平升高，且均与踝关节功能呈负相关性，并与疾病严重程度存在一定的关系。
Abstract:
Objective　To investigate the correlation between the changes of interleukin-1β (IL-1β) and matrix metalloproteinase-13 (MMP-13) levels in peripheral blood and joint function in patients with traumatic osteoarthritis after ankle fracture. Methods　93 patients with traumatic osteoarthritis after ankle fracture admitted in Department of Orthopedics of the 969th Hospital of Chinese PLA from September 2018 to June 2020 were selected as the study group, and the 79 healthy persons at the same period were selected as the control group. The levels of IL-1β and MMP-13 in peripheral blood were detected by enzyme linked immunosorbent assay in both groups and the ankle function [American Orthopedic Foot and Ankle Society (AOFAS) and Kofoed ankle score] were assessed and compared. The levels of IL-1β and MMP-13 in peripheral blood and ankle joint function score were also compared of the patients with different severity in the study group, and the correlation between the levels of IL-1β, MMP-13 and AOFAS score and Kofeed ankle score in the study group were analyzed by Pearson correlation analysis method. Results　The levels of IL-1β and MMP-13 in peripheral blood of the study group respectively was (50.36±7.82)pg/ml and (2.09±0.35)ng/L, which were higher than those of the control group（9.84±1.13 pg/ml，1.16±0.22 ng/L）, and the differences were statistically significant (t=45.632, 20.431, all P < 0.05). The AOFAS score and Kofoed ankle score of the study group respectively was (54.69±9.23) and (52.74±8.61) score，which were lower than those of the control group（90.25±6.27，89.06±7.34）, and the differences were statistically significant (t=29.019, 29.480, all P < 0.05). The levels of IL-1β and MMP-13 in peripheral blood of the moderate and severe patients were higher than those of the mild patients (t=16.229, 5.474；21.840, 11.862, all P < 0.05), of which the severe patients were higher than those of the moderate patients (t=12.300, 8.146, all P < 0.05)，the differences were statistically significant，respectively. The AOFAS score and Kofoed ankle score in moderate and severe patients were lower than those in mild patients (t=2.875, 3.233；7.444, 7.690, all P< 0.05), which in severe patients were lower than those in moderate patients (t=6.242, 5.840, all P< 0.05) ，the differences were statistically significant，respectively. Pearson correlation analysis showed that the levels of IL-1β and MMP-13 in peripheral blood of the patients in study group were negatively correlated with AOFAS score and Kofeed ankle score (r=-0.852, -0.846；-0.873, -0.868, all P < 0.05). Conclusion　The levels of IL-1β and MMP-13 in peripheral blood of patients with traumatic osteoarthritis after ankle fracture were increased, which were negatively correlated with ankle function, and all of them were related to the severity of the disease.

参考文献/References:

[1] 肖凯, 方真华, 李鲲, 等. 关节牵张成形术治疗踝关节创伤性骨关节炎[J].中华骨科杂志, 2017,37(10):604-610.
XIAO Kai, FANG Zhenhua, LI Kun, et al. Joint distraction arthroplasty as an alternative treatment for ankle osteoarthritis [J]. Chinese Journal of Orthopaedics, 2017, 37(10):604-610.
[2] SALAZAR-NORATTO G, DE NIJS N, STEVENS H Y, et al. Regional gene expression analysis of multiple tissues in an experimental animal model of posttraumatic osteoarthritis[J]. Osteoarthritis and Cartilage,2019, 27(2): 294-303.
[3] 刘佳. 前交叉韧带损伤后软骨退变和骨性关节炎发生的分子变化[J]. 基因组学与应用生物学, 2020,39(1)：394-401.
LIU Jia. Molecular changes of cartilage degeneration and osteoarthritis development with anterior cruciate ligament injury [J]. Genomics and Applied Biology,2020, 39(1):394-401.
[4] MORITAKE A, KAWAO N, OKADA K, et al.Plasminogen activator inhibitor-1 is involved in interleukin-1β-induced matrix metalloproteinase expression in murine chondrocytes[J]. Modern Rheumatology, 2019, 29(6): 959-963.
[5] SATISH K K, VELAYUTHAM R, ROY K K. A systematic computational analysis of human matrix metalloproteinase 13 (MMP-13) crystal structures and structure-based identification of prospective drug candidates as MMP-13 inhibitors repurposable for osteoarthritis[J]. Journal of Biomolecular Structure & Dynamics, 2020, 38(10): 3074-3086.
[6] 谢利民, 译. MOSKOWITZ R W, ALTMAN R D,HOCHBERG M C. 骨关节炎诊断与治疗 (精)[M]. 4版. 北京: 人民卫生出版社, 2008:31-32.
XIE Limin, Trans. MOSKOWITZ R W, ALTMAN R D, HOCHBERG M C. Diagnosis and treatment of osteoarthritis (essence) [M]. 4th Edition .Beijing:People’s Health Publishing House, 2008:31-32.
[7] VOSOUGHI A R, ROUSTAEI N, MAHDAVIAZAD H. American Orthopaedic Foot and Ankle Society ankle-hindfoot scale: A cross-cultural adaptation and validation study from Iran[J]. Foot and Ankle Surgery,2018, 24(3): 219-223.
[8] 李静, 刘依嘉, 姚冬英, 等. 关节镜技术联合超前多模式镇痛治疗踝关节创伤性骨关节炎的疗效[J]. 标记免疫分析与临床, 2019, 26(4): 550-554.
LI Jing, LIU Yijia, YAO Dongying, et al. The efficacy of arthroscopic technique combined with multimodal preemptive analgesia in the treatment of traumatic osteoarthritis of ankle joint [J]. Labeled Immunoassays and Clinical Medicine, 2019, 26(4):550-554.
[9] KELLGREN J H, LAWRENCE J S. Radiological assessment of rheumatoid arthrosis[J]. Annals of the Rheumatic Diseases, 1957, 16(4): 494-502.
[10] VERHAGE S M, KRIJNEN P, SCHIPPER I B, et al. Persistent postoperative step-off of the posterior malleolus leads to higher incidence of post-traumatic osteoarthritis in trimalleolar fractures[J]. Archives of Orthopaedic and Trauma Surgery, 2019, 139(3): 323-329.
[11] STONE A, GROL M W, RUAN M, et al. Combinatorial Prg4 and Il-1ra gene therapy protects against hyperalgesia and cartilage degeneration in Post-Traumatic osteoarthritis[J]. Human Gene Therapy,2019, 30(2): 225-235.
[12] BOKHARI R A, TANTOWI N, LAU S F, et al. Java tea(orthosiphon stamineus) protected against osteoarthritis by mitigating inflammation and cartilage degradation:a preclinical study[J]. Inflammopharmacology, 2018,26(4): 939-949.
[13] WIDOWATI W, AFIFAH E, MOZEF T, et al. Effects of insulin-like growth factor-induced Wharton jelly mesenchymal stem cells toward chondrogenesis in an osteoarthritis model[J]. Iranian Journal of Basic Medical Sciences, 2018, 21(7): 745-752.
[14] WONDIMU E B, CULLEY K L, QUINN J, et al. Elf3 contributes to cartilage degradation in vivo in a surgical model of Post-Traumatic osteoarthritis[J]. Scientific Reports, 2018, 8(1): 6438.
[15] PANINA S B, KROLEVETS I V, MILYUTINA N P,et al. Circulating levels of proinflammatory mediators as potential biomarkers of post-traumatic knee osteoarthritis development[J]. Journal of Orthopaedics and Traumatology, 2017, 18(4): 349-357.
[16] NAKAGAWA R, AKAGI R, YAMAGUCHI S, et al. Single vs.repeated matrix metalloproteinase-13 knockdown with intra-articular short interfering RNA administration in a murine osteoarthritis model[J].Connective Tissue Research, 2019, 60(4): 335-343.
[17] THOMAS N P, WU W J, FLEMING B C, et al.Synovial inflammation plays a greater role in posttraumatic osteoarthritis compared to idiopathic osteoarthritis in the Hartley Guinea pig knee[J]. BMC Musculoskeletal Disorders, 2017, 18(1): 556.
[18] SAITO M, NISHITANI K, IKEDA H O, et al. A VCP modulator, KUS121, as a promising therapeutic agent for post-traumatic osteoarthritis[J]. Scientific Reports,2020, 10(1): 20787.
[19] SUZUKI Y, HASEGAWA M, MATSUI Y, et al. Intraarticular injection of rebamipide prevents articular cartilage degeneration in murine post-traumatic osteoarthritis models[J]. Modern Rheumatology, 2020,30(4): 765-772.
[20] 樊凯, 张海严, 蔡道章.正五聚蛋白3 促进软骨细胞MMP13 产生从而加重骨性关节炎[J]. 实用医学杂志, 2020, 36(16):2177-2182.
FAN Kai, ZHANG Haiyan, CAI Daozhang. PTX3 promotes the production of MMP13 in chondrocytes and aggravates arthritis [J]. The Journal of Practical Medicine, 2020, 36(16):12-17.
[21] 赵娜, 康利宝. 创伤性膝骨关节炎患者血清IL-6,TNF-α, 瘦素水平表达与影像学K-L 分级的相关性[J]. 现代检验医学杂志, 2019, 34(5):139-142.
ZHAO Na, KANG Libao. Association between levels of serum inflammatory cytokines and imaging severity in patients with post-traumatic knee osteoarthritis[J]. Journal of Modern Laboratory Medicine, 2019,34(5):139-142.
[22] 毛洪刚, 朱艳丽, 刘岩, 等.膝骨性关节炎者TLR-4，IL-1β 的表达变化及临床意义[J].中国医药导报,2020, 17(3):82-85.
MAO Honggang, ZHU Yanli, LIU Yan, et al.Expression changes and clinical significance of TLR-4 and IL-1β in serum and knee joint fluid of patients with knee osteoarthritis [J]. China Medical Herald,2020, 17(3):82-85.
[23] 骆晓飞, 王金良, 魏瑄, 等. 血清及关节液TNF-α和MMP-13 水平变化与膝骨性关节炎患者K-L 分级的相关性[J]. 中国卫生工程学, 2020, 19(1): 100-102.
LUO Xiaofei, WANG Jinliang, WEI Xuan, et al.Correlation between changes of TNF-α and MMP-13 levels in serum and synovial fluid and K-L grade in patients with knee osteoarthritis[J]. Chinese Journal of Public Health Engineering, 2020, 19(1):100-102.

备注/Memo

备注/Memo:
基金项目：全军医学科技青年培育项目：踝关节失稳及稳定性重建与关节软骨退变的试验研究及初步临床观察（13QNP014）。
作者简介：姜万嵩（1978-），男，本科，主管技师，从事临床检验和临床输血，E-mail：997238501@qq.com。
通讯作者：马骁（1979-），男，博士，副主任医师，从事骨科运动医学研究，E-mail：doctorma253@163.com。
更新日期/Last Update: 2022-09-15