[1]张莉娜,张霁雯,罗 酩,等.胰腺癌组织CTTN 和miR-545-3p 表达水平及其与临床病理和预后的相关性研究[J].现代检验医学杂志,2022,37(06):99-103+118.[doi:10.3969/j.issn.1671-7414.2022.06.018]
　ZHANG Li-na,ZHANG Ji-wen,LUO Ming,et al.Expression Levels of CTTN and miR-545-3p in Pancreatic Cancer Tissues and Their Correlation with Clinicopathology and Prognosis[J].Journal of Modern Laboratory Medicine,2022,37(06):99-103+118.[doi:10.3969/j.issn.1671-7414.2022.06.018]
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胰腺癌组织CTTN 和miR-545-3p 表达水平及其与临床病理和预后的相关性研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第37卷
期数:
2022年06期
页码:
99-103+118
栏目:
论著
出版日期:
2022-11-15

文章信息/Info

Title:
Expression Levels of CTTN and miR-545-3p in Pancreatic Cancer Tissues and Their Correlation with Clinicopathology and Prognosis
文章编号:
1671-7414(2022)06-099-06
作者:
张莉娜1; 张霁雯1; 罗 酩1; 赵苏鸣2
(1. 上海市宝山区罗店医院消化内科，上海 201900；2. 南通大学附属医院消化内科， 江苏南通 226001)
Author(s):
ZHANG Li-na1;  ZHANG Ji-wen1;  LUO Ming1;  ZHAO Su-ming2
(1. Department of Gastroenterology，Luodian Hospital of Baoshan District, Shanghai 201900, China； 2. Department of Gastroenterology, Affiliated Hospital of Nantong University, Jiangsu Nantong 226001, China)
关键词:
胰腺癌; 皮层肌动蛋白; 微小核糖核酸-545-3p
分类号:
R735.9；R730.43
DOI:
10.3969/j.issn.1671-7414.2022.06.018
文献标志码:
A
摘要:
目的 探究胰腺癌组织中皮层肌动蛋白(cortical actin，CTTN)、微小核糖核酸(micro RNA, miR)-545-3p的表达情况及其与患者预后的相关性。方法 募集2016 年1 月～ 2018 年12 月南通大学附属医院行腹腔镜胰腺癌切除手术的胰腺癌患者138 例，采集胰腺癌组织和癌旁正常组织，通过实时荧光定量PCR(real-time quantitative PCR, qRTPCR)检测CTTN mRNA 和miR-545-3p 表达，二者的相关性通过Pearson 法进行分析，分析CTTN mRNA 和miR-545-3p 表达与临床病理特征的关系，绘制Kapplan-Meier 生存曲线分析胰腺癌组织中CTTN mRNA 和miR-545-3p 表达与患者生存率的关系，经COX 回归对影响胰腺癌患者生存的危险因素进行分析。结果 与癌旁正常组织比较，胰腺癌组织中CTTN mRNA 表达上调(2.11±0.87 vs 1.02±0.46)，miR-545-3p 表达下调(0.45±0.19 vs 0.97±0.42), 差异均有统计学意义(t=12.895，13.251，均P=0.000)；胰腺癌组织中CTTN mRNA 和miR-545-3p 之间具有负相关性(r=-0.410，P ＜ 0.05)；CTTN mRNA 和miR-545-3p 表达均与肿瘤分化程度、TNM 分期以及淋巴结转移有关(t=2.473 ～ 11.418，均P ＜ 0.05)。根据CTTN mRNA 和miR-545-3p 表达量的均值将所有患者依次分为CTTN mRNA 高表达组和CTTNmRNA 低表达组，miR-545-3p 高表达组和miR-545-3p 低表达组。生存曲线分析得出，CTTN mRNA 低表达组三年生存率高于CTTN mRNA 高表达组(24.62% vs 9.59%)，miR-545-3p 高表达组三年生存率高于miR-545-3p 低表达组(25.37%vs 8.45%), 差异均有统计学意义(χ2=4.560，P=0.033；χ2=5.941，P=0.015)。存在淋巴结转移、高CTTN mRNA 表达、低miR-545-3p 表达是胰腺癌患者术后三年死亡的危险因素(Waldχ2=1.885, 1.562, 0.625，均P ＜ 0.05)。结论 胰腺癌组织中CTTN mRNA 高表达和miR-545-3p 低表达均与患者预后不良有关。
Abstract:
Objective To investigate the expressions of cortical actin (CTTN) and micro RNA(miR)545-3p in pancreatic cancer tissues and their correlation with the prognosis of patients. Methods A total of 138 pancreatic cancer patients who underwent laparoscopic pancreatic cancer resection in Affiliated Hospital of Nantong University from January 2016 to December 2018 were recruited, and their pancreatic cancer tissues and adjacent normal tissues were collected. The expressions of CTTN mRNA and miR-545-3p were measured by qRT-PCR, and the correlation between the two was analyzed by Pearson method. The relationship between CTTN mRNA, miR-545-3p expression and clinicopathological features was analyzed, Kapplan-Meier survival curve was drawn to analyze the relationship between CTTN mRNA and miR-545-3p expressions in pancreatic cancer tissue and the survival rate of patients, and the risk factors affecting the survival of pancreatic cancer patients were analyzed by COX regression. Results Compared with adjacent normal tissues, the expression of CTTN mRNA in pancreatic cancer tissues was up-regulated (2.11±0.87 vs 1.02±0.46), and the expression of miR-545-3p was down-regulated (0.45±0.19 vs 0.97±0.42),the differences were statistically significant(t=12.895, 13.251, all P=0.000).There was a negative correlation between CTTN mRNA and miR-545-3p in pancreatic cancer tissue (r=-0.410, P<0.05).The expressions of CTTN mRNA and miR-545-3p were related to tumor differentiation, TNM stage and lymph node metastasis (t=2.473 ～ 11.418，all P ＜ 0.05).According to the mean of CTTN mRNA and miR-545-3p expressions, all patients were divided into CTTN mRNA high expression group and CTTN mRNA low expression group, miR-545-3p high expression group and miR-545-3p low expression group. Survival curve analysis showed that the 3-year survival rate of CTTN mRNA low expression group was higher than that of CTTN mRNA high expression group (24.62% vs 9.59%), and the 3-year survival rate of miR-545-3p high expression group was higher than that of miR-545-3p low expression group (25.37% vs 8.45%) ,the differences were statistically significant(χ2=4.560, P=0.033, χ2=5.941, P=0.015). Lymph node metastasis, high CTTN mRNA expression, and low miR-545-3p expression were risk factors for 3-year postoperative death in pancreatic cancer patients (Waldχ2=1.885, 1.562, 0.625, all P ＜ 0.05). Conclusion The expression of CTTN mRNA was high and the expression of miR-545-3p was low in pancreatic cancer tissue, and they would be related to the poor prognosis of patients.

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备注/Memo

备注/Memo:
基金项目：南通市科技项目(项目编号：MS12018066)。
作者简介：张莉娜(1987-)，女，硕士研究生，住院医师，从事胰腺癌早期诊断方面研究，E-mail：zl19876n@163.com。
更新日期/Last Update: 2022-11-15