[1]包秋红,贾海玉,张 勇,等.射血分数保留性心力衰竭患者血清miR-146a，IRAK-1 表达水平与血管内皮功能、心室重构的相关性分析[J].现代检验医学杂志,2023,38(03):109-113+127.[doi:10.3969/j.issn.1671-7414.2023.03.019]
　BAO Qiu-hong,JIA Hai-yu,ZHANG Yong,et al.Correlation Analysis of Serum miR-146a, IRAK-1 Expression Levels with Vascular Endothelial Function and Ventricular Remodeling in Patients with Ejection Fraction Preserved Heart Failure[J].Journal of Modern Laboratory Medicine,2023,38(03):109-113+127.[doi:10.3969/j.issn.1671-7414.2023.03.019]
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射血分数保留性心力衰竭患者血清miR-146a，IRAK-1 表达水平与血管内皮功能、心室重构的相关性分析()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第38卷
期数:
2023年03期
页码:
109-113+127
栏目:
论著
出版日期:
2023-05-15

文章信息/Info

Title:
Correlation Analysis of Serum miR-146a, IRAK-1 Expression Levels with Vascular Endothelial Function and Ventricular Remodeling in Patients with Ejection Fraction Preserved Heart Failure
文章编号:
1671-7414（2023）03-109-06
作者:
包秋红; 贾海玉; 张 勇; 曹中朝; 刘 昀
（内蒙古医科大学附属医院老年医学中心，呼和浩特 010010）
Author(s):
BAO Qiu-hong;  JIA Hai-yu;  ZHANG Yong;  CAO Zhong-zhao;  LIU Yun
（Center for Geriatric Medicine，the Affiliated Hospital of Inner Mongolia Medical University，Hohhot 010010, China）
关键词:
射血分数保留性心力衰竭; 微小核糖核酸-146a; 白介素1 受体相关激酶1; 血管内皮功能; 心室重构
分类号:
R541.6；R392.11
DOI:
10.3969/j.issn.1671-7414.2023.03.019
文献标志码:
A
摘要:
目的 探讨射血分数保留性心力衰竭（heart failure with preserved ejection fraction，HFpEF）患者血清微小RNA（micro RNA，miR）-146a、白介素1 受体相关激酶1（interleukin-1 receptor-associated kinase 1，IRAK-1）表达水平及其与血管内皮功能、心室重构的相关性。方法 选择2021 年6 月～ 2022 年3 月在内蒙古医科大学附属医院收治的93例HFpEF 患者为观察对象，选择同期门诊体检健康者（无心血管病史）90 例为对照组，收集患者资料，采用实时荧光定量PCR（quantitative real-time PCR，qRT-PCR）检测血清miR-146a和IRAK-1 mRNA表达，采用酶联免疫吸附试验（enzymelinked immunosorbent assay，ELISA）法检测血清内皮素-1（endothelin-1，ET-1）水平，化学发光法检测血清一氧化氮（nitric oxide，NO）水平，超声心动图检查左室射血分数（LVEF）、二尖瓣口舒张早期最大血流速度E 峰与舒张晚期最大血流速度A 峰的比值（E/A）、左心室舒张末期内径（LVEDD）、左心房内径（LAD）和左室质量指数（LVMI）。Pearson 法分析HFpEF 患者血清miR-146a 和IRAK-1 mRNA 表达水平的相关性及miR-146a，IRAK-1 与血管内皮功能、心室重构相关性指标的相关性，ROC 曲线分析血清miR-146a 和IRAK-1 对HFpEF 的诊断价值。结果 与对照组比较，HFpEF 组血清miR-146a（2.13±0.35 vs 1.05±0.21）表达水平升高，IRAK-1 mRNA（0.65±0.10 vs 1.03±0.12）表达水平降低，差异有统计学意义（t=25.209，23.302，均P ＜ 0.05）；HFpEF 患者血清miR-146a 与IRAK-1 mRNA 水平呈负相关（r=-0.473，P ＜ 0.001），血清miR-146a 与ET-1，LVEDD，LAD，LVMI 呈正相关（r=0.501，0.556，0.391，0.601，均P ＜ 0.001），与血清NO，LVEF，E/A 呈负相关（r=-0.453，-0.623，-0.613，均P ＜ 0.001）；IRAK-1 mRNA 与血清ET-1，LVEDD，LAD，LVMI 呈负相关（r=-0.369，-0.478，-0.551，-0.457， 均P ＜ 0.001）， 与血清NO，LVEF，E/A 呈正相关（r=0.447，0.605，0.567，均P ＜ 0.001）。血清miR-146a，IRAK-1 联合诊断HFpEF 的AUC 显著高于miR-146a，IRAK-1 单独诊断（Z=2.122，2.067，P=0.033，0.038）。结论 HFpEF 患者血清miR-146a 表达水平升高，IRAK-1 表达水平降低，与血管内皮功能、心室重构相关，可能成为HFpEF 诊断与病情评估的指标。
Abstract:
Objective To investigate the expression levels of serum microRNA (miR) -146a and interleukin-1 receptorassociated kinase 1 (IRAK-1) in patients with heart failure with preserved ejection fraction (HFpEF) and their correlation with vascular endothelial function and ventricular remodeling. Methods 93 HFpEF patients admitted to the Affiliated Hospital of Inner Mongolia Medical University from June 2021 to March 2022 were selected as observation objects. A total of 90 healthy people (with no history of cardiovascular disease) were selected as the control group. The data of patients were collected, Quantitative Real-time PCR（qRT-PCR） was used to detect the expression of serum miR-146a and IRAK-1 mRNA, Enzyme linked immunosorbent assay （ELISA）was used to detect the level of serum endothelin-1 (ET-1), chemiluminescent immunoassay methods were used to detect the level of serum nitric oxide(NO), echocardiography was used to examine left ventricular ejection fraction (LVEF), the ratio of the maximum early diastolic flow velocity of the mitral valve E-peak to the maximum late-diastolic flow velocity of the A-peak (E/A), the left ventricular end-diastolic diameter (LVEDD), left atrial diameter (LAD), and left ventricular mass index (LVMI). Pearson was used to analyze the correlation of serum miR-146a and IRAK-1 mRNA expression levels in HFpEF patients and the correlation of miR-146a and IRAK-1 with vascular endothelial function and ventricular remodeling-related indicators, ROC curve was used to analyze the diagnostic value of serum miR-146a and IRAK-1 for HFpEF. Results Compared with the control group, the expression level of miR-146a (2.13±0.35 vs 1.05±0.21) in serum of HFpEF group was increased, and the expression level of IRAK-1 mRNA (0.65±0.10 vs 1.03±0.12) was decreased, with statistical significance (t=25.209, 23.302, all P<0.05). Serum miR-146a and IRAK-1 levels in HFpEF patients were negatively correlated (r=-0.473, P<0.001). Serum miR-146a was positively correlated with ET-1, LVEDD, LAD and LVMI (r=0.501, 0.556, 0.391, 0.601, all P<0.001), negatively correlated with serum NO, LVEF and E/A (r=-0.453, -0.623, -0.613, all P<0.001), IRAK-1 mRNA was negatively correlated with serum ET-1, LVEDD, LAD and LVMI (r=-0.369, -0.478, -0.551, -0.457, all P<0.001), and positively correlated with serum NO, LVEF and E/A (r=0.447, 0.605, 0.567, all P ＜ 0.001). The AUC of serum miR-146a and IRAK-1 in the diagnosis of HFpEF was significantly higher than that of miR-146a and IRAK-1 alone (Z=2.122, 2.067, P=0.033, 0.038). Conclusion The expression level of serum miR-146a in HFpEF patients was increased, and the expression level of IRAK-1 was decreased, and may become an indicator of diagnosis and disease evaluation of HFpEF.

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备注/Memo

备注/Memo:
基金项目： 2022 年度内蒙古自治区卫生健康科技计划项目（编号：202201239）：沙库巴曲缬沙坦对射血分数保留性心衰患者心肌纤维化和血管内皮功能的影响。
作者简介：包秋红（1982-），女，主任医师，研究方向：心血管内科疾病的诊断及治疗，E-mail ：bennet_y@yeah.net。
通讯作者：贾海玉（1970-），女，主任医师，研究方向：心血管内科疾病的诊断及治疗， E-mail：peppe207@163.com.。
更新日期/Last Update: 2023-05-15