[1]王 飞,祝 靳,赵宝林.溃疡性结肠炎患者血清LncRNA Mirt2 和LncRNA IFNG-AS1 表达水平及与预后相关性研究[J].现代检验医学杂志,2023,38(03):114-118+148.[doi:10.3969/j.issn.1671-7414.2023.03.020]
　WANG Fei,ZHU Jin,ZHAO Bao-lin.Study on the Expression Levels of Serum LncRNA Mirt2 and LncRNA IFNGAS1 in Patients with Ulcerative Colitis and Their Correlation with Prognosis[J].Journal of Modern Laboratory Medicine,2023,38(03):114-118+148.[doi:10.3969/j.issn.1671-7414.2023.03.020]
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溃疡性结肠炎患者血清LncRNA Mirt2 和LncRNA IFNG-AS1 表达水平及与预后相关性研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第38卷
期数:
2023年03期
页码:
114-118+148
栏目:
论著
出版日期:
2023-05-15

文章信息/Info

Title:
Study on the Expression Levels of Serum LncRNA Mirt2 and LncRNA IFNGAS1 in Patients with Ulcerative Colitis and Their Correlation with Prognosis
文章编号:
1671-7414（2023）03-114-06
作者:
王 飞; 祝 靳; 赵宝林
（南京市浦口区中医院/ 南京市中医院浦口分院肛肠科，南京 211800）
Author(s):
WANG Fei;  ZHU Jin;  ZHAO Bao-lin
（Department of Anorectal, Nanjing Pukou District Traditional Chinese Medicine Hospital/Nanjing Traditional Chinese Medicine Hospital Pukou Branch, Nanjing 211800, China）
关键词:
溃疡性结肠炎; 长链非编码RNA 心肌梗死相关转录本2; 长链非编码RNA 干扰素γ- 反义RNA1
分类号:
R574.62；R392.11
DOI:
10.3969/j.issn.1671-7414.2023.03.020
文献标志码:
A
摘要:
目的 探讨长链非编码RNA心肌梗死相关转录本2（long non-coding RNA myocardial infarction-related transcript 2，LncRNA Mirt2）、长链非编码RNA 干扰素γ- 反义RNA1（long non-coding RNA interferon γ-antisense RNA 1，LncRNA IFNG-AS1）在溃疡性结肠炎（ulcerative colitis，UC）患者血清中表达情况及与UC 患者预后相关性。方法 选择2018 年12 月～ 2021 年1 月南京市浦口区中医院（南京市中医院浦口分院）收治的193 例UC 患者作为观察对象，其中缓解期85 例，活动期108 例。根据患者预后情况分为复发组（n=78）和未复发组（n=115），另选取同期190例健康体检者作为对照组。采用实时荧光定量PCR（quantitative real-time PCR，qRT-PCR）检测血清LncRNA Mirt2 和LncRNA IFNG-AS1 表达水平，Pearson 法分析UC 患者血清LncRNA Mirt2 和LncRNA IFNG-AS1 表达水平相关性及LncRNA Mirt2 和LncRNA IFNG-AS1 与C- 反应蛋白（C-reactive protein，CRP）、白细胞介素-6 (interleukin- 6) 和肿瘤坏死因子-α(tumor necrosis factor- α，TNF-α) 的相关性；ROC 曲线分析血清LncRNA Mirt2 和LncRNA IFNG-AS1 对复发UC 的诊断价值。结果 与对照组比较，缓解期组与活动期组UC 患者血清LncRNA Mirt2（0.92±0.10，0.71±0.08vs 1.07±0.12）表达水平降低，LncRNA IFNG-AS1（1.63±0.25，2.27±0.42 vs 1.01±0.14）表达水平及CRP（13.42±4.71mg/L,25.38±6.29 mg/L vs 4.32±1.27 mg/L），IL-6（8.31±2.35pg/ml,16.55±4.52 pg/ml vs 2.87±0.78 pg/ml），TNF-α（15.38±4.29 pg/ml,28.94±6.37 pg/ml vs 8.42±1.63 pg/ml）水平升高，差异具有统计学意义（t=10.064 ～ 44.632，均P<0.001）；与缓解期组相比，活动期组UC 患者血清LncRNA Mirt2 表达水平降低，LncRNA IFNG-AS1 表达水平及CRP，IL-6，TNF-α 水平升高，差异具有统计学意义（t=12.420 ～ 16.844，均P<0.001）；与轻度组比较，中、重度组UC 患者血清LncRNA Mirt2（0.69±0.08，0.58±0.05 vs 0.81±0.06）表达水平降低，LncRNA IFNG-AS1（2.25±0.48，2.83±0.25 vs 1.90±0.31）表达水平升高，差异有统计意义（t=3.890 ～ 17.225，均P<0.001）；与中度组比较，重度组UC 患者血清LncRNA Mirt2 表达水平降低，LncRNA IFNG-AS1 表达水平升高，差异具有统计学意义（t=6.184，5.957，均P<0.001）。相关性分析显示，UC 患者血清LncRNA Mirt2 表达水平与CRP，IL-6 和TNF-α 呈负相关（r=-0.623，-0.605，-0.571，均P<0.001），LncRNA IFNG-AS1 表达水平与CRP，IL-6 和TNF-α 呈正相关（r=0.457，0.531，0.497，均P<0.001）。与未复发组比较，复发组UC 患者血清LncRNA Mirt2（0.70±0.08 vs 0.87±0.11）表达水平显著降低，LncRNA IFNG-AS1（2.38±0.41 vs 1.72±0.28）表达水平显著升高（t=11.706，13.294，均P<0.001）；血清LncRNAMirt2，LncRNA IFNG-AS1 联合诊断复发UC 的曲线下面积为0.931（95%CI：0.886 ～ 0.963），敏感度和特异度分别为87.18%，86.96%；阳性预测值、阴性预测值和准确度分别为81.93%，90.91% 和87.05%。结论 UC 患者血清LncRNAMirt2 表达水平降低、LncRNA IFNG-AS1 表达水平升高，与炎症水平和患者预后有关，可作为反映UC 患者病情与预后的标志物。
Abstract:
Objective To investigate the expression of long non-coding RNA myocardial infarction-related transcript 2 (LncRNA Mirt2) and long non-coding RNA interferon γ-antisense RNA 1 (LncRNA IFNG-AS1) in serum of patients with ulcerative colitis (UC) and their correlation with prognosis of UC patients. Methods A total of 193 UC patients admitted to Nanjing Pukou District Traditional Chinese Medicine Hospital (Nanjing Traditional Chinese Medicine Hospital Pukou Branch) from December 2018 to January 2021 were selected as observation objects including 85 cases in remission stage, 108 cases in active stage. According to the prognosis of patients, they were grouped into a recurrence group （n=78）and a non-recurrence group （n=115）. In addition, 190 healthy subjects were taken as the control group. The expression levels of serum LncRNA Mirt2 and LncRNA IFNG-AS1 were measured by Quantitative Real-time PCR (qRT-PCR) , the correlation between serum LncRNA Mirt2, LncRNA IFNG-AS1 expression levels, LncRNA Mirt2, LncRNA IFNG-AS1 and C-reactive protein , Interleukin-6(IL-6) and Tumor necrosis factor-α (TNF-α) were analyzed by Pearson method, and the diagnostic value of serum LncRNA Mirt2 and LncRNA IFNG-AS1 in recurrent UC was analyzed by ROC curve. Results Compared with the control group, the expression levels of serum LncRNA Mirt2 (0.92±0.10, 0.71±0.08 vs 1.07±0.12) in UC patients in the remission and active groups were decreased, LncRNA IFNG-AS1 (1.63±0.25, 2.27±0.42 vs 1.01±0.14) and CRP（13.42±4.71mg/L, 25.38±6.29 mg/L vs 4.32±1.27 mg/L）, IL-6（8.31±2.35pg/ml,16.55±4.52 pg/ml vs 2.87±0.78 pg/ml） and TNF-α（15.38±4.29 pg/ml, 28.94±6.37 pg/ml vs 8.42±1.63 pg/ml）levels increased, and the differences were statistically significant (t=10.064 ～ 44.632, all P<0.001）.Compared with the remission group, the serum LncRNA Mirt2 expression level of UC patients in the active group was decreased (0.71±0.08 vs 0.92±0.10), LncRNA IFNG-AS1 (2.27±0.42 vs 1.63±0.25) expression level and CRP, IL-6 and TNF-α levels increased, and the differences were statistically significant (t=12.420 ～ 16.844, all P<0.001). Compared with the mild group, the expression levels of serum LncRNA Mirt2 (0.69±0.08, 0.58±0.05 vs 0.81±0.06) in the moderate and severe UC patients were decreased, the expression level of LncRNA IFNG-AS1 (2.25±0.48, 2.83±0.25 vs 1.90±0.31) was increased, and the differences were statistically significant (t=3.890 ～ 17.225, all P<0.001). Compared with the moderate group, the severe the expression level of serum LncRNA Mirt2 in the UC patients in the group decreased, and the expression level of LncRNA IFNG-AS1 was increased, and the differences were statistically significant (t=6.184, 5.957, all P<0.001). Correlation analysis showed that serum LncRNA Mirt2 expression levels in UC patients were negatively correlated with CRP, IL-6 and TNF-α (r=- 0.623, -0.605, -0.571, all P<0.001), the expression level of LncRNA IFNG-AS1 was positively correlated with CRP, IL-6 and TNF-α (r=0.457, 0.531, 0.497, all P<0.001). Compared with the non-relapsed group, the serum LncRNA Mirt2 (0.70±0.08 vs 0.87±0.11) expression level of UC patients in the relapsed group was significantly decreased, and LncRNA IFNG-AS1 (2.38±0.41 vs 1.72±0.28) expression level was significantly increased (t=11.706, 13.294, all P<0.001). The area under the curve of serum LncRNA Mirt2 and LncRNA IFNG-AS1 for the diagnosis of recurrent UC was 0.931(95%CI: 0.886 ～ 0.963), the sensitivity, specificity were 87.18%, 86.96%, respectively, and the positive predictive value, negative predictive value, accuracy were 81.93%, 90.91% and 87.05%，respectively. Conclusion The expression level of LncRNA Mirt2 in serum of UC patients was decreased, and the expression level of LncRNA IFNG-AS1 was increased. They were related to the level of inflammation and the prognosis of patients, and may be used as markers to reflect the condition and prognosis of UC patients.

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备注/Memo

备注/Memo:
基金项目：南京市浦口区社会事业科技发展计划项目（S2021-22）。
作者简介：王飞（1979-），男，硕士研究生，副主任中医师，主要从事中西医结合肛肠病临床研究，E-mail：wangfei123wf@126.com。
通讯作者：祝靳，E-mail：1225875512@qq.com。
更新日期/Last Update: 2023-05-15