[1]赵 萍a,吴红霞b,高海珍b.梅毒血清固定患者外周血单个核淋巴细胞中miR-31 和miR-192 水平表达及其诊断价值研究[J].现代检验医学杂志,2023,38(05):29-33+46.[doi:10.3969/j.issn.1671-7414.2023.05.006]
　ZHAO Pinga,WU Hongxiab,GAO Haizhenb.Study on the Expression Levels and Diagnostic Value of miR-31 and miR-192 in Peripheral Blood Mononuclear Lymphocytes of Patients with Syphilis Serum Fixation[J].Journal of Modern Laboratory Medicine,2023,38(05):29-33+46.[doi:10.3969/j.issn.1671-7414.2023.05.006]
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梅毒血清固定患者外周血单个核淋巴细胞中miR-31 和miR-192 水平表达及其诊断价值研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第38卷
期数:
2023年05期
页码:
29-33+46
栏目:
论著
出版日期:
2023-09-15

文章信息/Info

Title:
Study on the Expression Levels and Diagnostic Value of miR-31 and miR-192 in Peripheral Blood Mononuclear Lymphocytes of Patients with Syphilis Serum Fixation
文章编号:
1671-7414（2023）05-029-06
作者:
赵 萍a; 吴红霞b; 高海珍b
（江阴市人民医院a．皮肤科；b．检验科，江苏江阴 214400）
Author(s):
ZHAO Pinga; WU Hongxiab; GAO Haizhenb
（a. Department of Dermatology; b. Department of Clinical Laboratory, Jiangyin People’s Hospital, Jiangsu Jiangyin 214400, China）
关键词:
微小核糖核酸-31; 微小核糖核酸-192; 梅毒血清固定
分类号:
R377.1：R392.11
DOI:
10.3969/j.issn.1671-7414.2023.05.006
文献标志码:
A
摘要:
目的 探讨梅毒血清固定患者外周血单个核淋巴细胞中微小核糖核酸（micro RNA，miR）-31 和微小核糖核酸（micro RNA，miR）-192 的表达水平及其诊断价值。方法 选取2020 年11 月～ 2022 年11 月江阴市人民医院收治的梅毒患者81 例，根据患者进行驱梅治疗后的血清转变情况分为血清固定组（n=37）和转阴组（n=44），根据临床病理分期分为一期梅毒患者组（n=45）和二期梅毒患者组（n=36），根据梅毒快速血浆反应素试验（rapid plasma responsetest，RPR）分为反应素初始滴度＜ 1∶8 梅毒患者组（n=31），1∶8 ～ 1∶32 梅毒患者组（n=26）和＞ 1∶32 梅毒患者组（n=24）。另选取同期进行健康体检的50 例志愿者为健康对照组。实时荧光定量PCR 检测各组外周血miR-31 和miR-192 水平；Logistic 回归分析梅毒患者发生血清固定的影响因素；受试者工作特征（receiver operating characteristic，ROC）曲线分析外周血miR-31，miR-192 水平对梅毒患者发生血清固定的诊断价值。结果 血清固定组、转阴组和健康对照组外周血miR-31（0.36±0.10，0.68±0.13，1.01±0.22）和miR-192（0.51±0.12，0.73±0.19，1.03±0.05）表达水平逐渐升高，差异具有统计学意义（F=167.815，173.090，均P ＜ 0.05）；病理分期为Ⅰ期患者外周血miR-31（0.56±0.13），miR-192（0.76±0.14）水平显著高于Ⅱ期（0.38±0.10，0.47±0.08），差异具有统计学意义（t=6.842，11.059，均P ＜ 0.05）；反应素初始滴度＜ 1∶8、反应素初始滴度为1∶8 ～ 1∶32、反应素初始滴度＞ 1∶32 的梅毒患者血清miR-31（0.56±0.18，0.48±0.12，0.39±0.09）和miR-192（0.80±0.20，0.64±0.13，0.49±0.12）表达水平逐渐降低，差异具有统计学意义（F=10.057，26.166，均P ＜ 0.05）；miR-31[OR(95%CI)=0.537(0.338 ～ 0.853)] 和miR-192 [OR(95%CI)=0.375(0.192 ～0.732)] 均是梅毒患者发生血清固定的保护因素（均P ＜ 0.05）；外周血miR-31，miR-192 以及两者联合诊断梅毒患者发生血清固定的曲线下面积（area under curve，AUC）分别为0.729（95%CI：0.618 ～ 0.821），0.821（95%CI：0.720 ～ 0.898）和0.927（95%CI：0.847 ～ 0.973），联合诊断的敏感度和特异度分别为83.78%，84.09%，二者联合优于外周血miR-31 和miR-192 各自单独诊断，差异具有统计学意义（Z=3.836，2.563，均P ＜ 0.05）。结论 外周血miR-31 和miR-192 在梅毒血清固定患者体内下调，二者联合诊断梅毒血清固定具有较高效能。
Abstract:
Objective To investigate the expression level and diagnostic value of microRNA (miR) -31and microRNA (miR) -192 in peripheral blood mononuclear lymphocytes of patients with Syphilis serum fixation. Methods A total of 81 Syphilis patients admitted to Jiangyin People’s Hospital from November 2020 to November 2022 were collected. According to the serum transformation status after anti plum therapy, they were divided into a serum fixation group (n=37) and a negative conversion group (n=44). According to clinical pathological staging, there were divided into a primary Syphilis group (n=45) and a secondary Syphilis group (n=36). According to the rapid plasma response test (RPR) for Syphilis, there were divided into an initial titer < 1∶8 (n=31), an initial titer of 1∶8 ～ 1∶32 (n=26)，and an initial titer > 1∶32 (n=24)．Another 50 volunteers who underwent physical examinations in the hospital during the same period were collected as the healthy control group. Real-time fluorescence quantitative PCR was used to detect the levels of miR-31 and miR-192 in peripheral blood of each group. Logistic regression was applied to analyze the influencing factors of serum fixation in Syphilis patients. Receiver operating characteristic (ROC) curve was applied to analyze the diagnostic value of peripheral blood miR-31 and miR-192 levels for serum fixation in Syphilis patients. Results The expression levels of miR-31 (0.36±0.10, 0.68±0.13, 1.01±0.22) and miR-192 (0.51±0.12, 0.73±0.19, 1.03±0.05) in peripheral blood of serum fixation group, negative conversion group and healthy control group were gradually increased, and the differences were statistically significant (F=167.815, 173.090, all P<0.05). The levels of miR-31 (0.56±0.13) and miR-192 (0.76±0.14) in peripheral blood of patients with pathological stage I were significantly higher than those of patients with stage II (0.38±0.10, 0.47±0.08), and the differences were statistically significant (t=6.842, 11.059, all P<0.05). The levels of serum miR-31 (0.56±0.18, 0.48±0.12, 0.39±0.09) and miR-192 (0.80±0.20, 0.64±0.13, 0.49±0.12) in Syphilis patients with initial titer < 1∶8，initial titer of 1∶8 ～ 1∶32，and initial titer >1∶32 gradually decreased, and the differences were statistically significant (F=10.057, 26.166, all P<0.05). Both miR-31 [OR (95%CI)=0.537 (0.338 ～ 0.853)] and miR-192 [OR (95%CI)=0.375 (0.192 ～ 0.732)] were protective factors for serum fixation in patients with Syphilis (P<0.05). The AUC of peripheral blood miR-31, miR-192, and their combination in the diagnosis of serum fixation in Syphilis patients was 0.729 (95%CI: 0.618 ～ 0.821), 0.821 (95%CI: 0.720 ～ 0.898) and 0.927 (95%CI: 0.847 ～ 0.973) respectively, and the sensitivity and specificity of the combined diagnosis were 83.78% and 84.09% respectively. The combination of the two was superior to the individual diagnosis of peripheral blood miR-31 and miR-192, and the differences were statistically significant (Z=3.836，2.563, all P<0.05). Conclusion Peripheral blood miR-31 and miR-192 were down-regulated in patients with Syphilis serum fixation, and the combination of the two has high efficacy in diagnosing Syphilis serum fixation.

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备注/Memo

备注/Memo:
作者简介：赵萍(1978-)，女，本科，副主任医师，研究方向：荨麻疹、脱发、寻常疣、梅毒、尖锐湿疣等疾病，E-mail：rud94j@163.com。
更新日期/Last Update: 2023-09-15