[1]高继英a,石代乐b,王 刚c.缺血性脑卒中患者血清LncRNA SNHG8 的表达及临床意义[J].现代检验医学杂志,2023,38(05):75-79.[doi:10.3969/j.issn.1671-7414.2023.05.014]
　GAO Jiyinga,SHI Daileb,WANG Gangc.Expression and Clinical Significance of Serum LncRNA SNHG8 in Patients with Ischemic Stroke[J].Journal of Modern Laboratory Medicine,2023,38(05):75-79.[doi:10.3969/j.issn.1671-7414.2023.05.014]
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缺血性脑卒中患者血清LncRNA SNHG8 的表达及临床意义()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第38卷
期数:
2023年05期
页码:
75-79
栏目:
论著
出版日期:
2023-09-15

文章信息/Info

Title:
Expression and Clinical Significance of Serum LncRNA SNHG8 in Patients with Ischemic Stroke
文章编号:
1671-7414（2023）05-075-05
作者:
高继英a; 石代乐b; 王 刚c
（河北北方学院附属第一医院 a. 神经外科高压氧舱室；b. 神经外科；c. 神经内科，河北张家口 075000）
Author(s):
GAO Jiyinga; SHI Daileb; WANG Gangc
（a. Hyperbaric Oxygen Chamber Room of Neurosurgery Department; b. Department of Neurosurgery; c. Department of Neurology, the First Affiliated Hospital of Hebei North University, Hebei Zhangjiakou 075000, China）
关键词:
长链非编码核糖核酸; 小核仁核糖核酸宿主基因8; 缺血性脑卒中
分类号:
R743.3；R392.11
DOI:
10.3969/j.issn.1671-7414.2023.05.014
文献标志码:
A
摘要:
目的 探讨长链非编码RNA 小核仁RNA 宿主基因8（long non-coding RNA small nucleolar RNA host gene 8，LncRNA SNHG8）在缺血性脑卒中（ischemic stroke，IS）患者血清中的表达水平及临床意义。方法 选择2019 年5 月～ 2021年12 月在河北北方学院附属第一医院神经内科住院的IS 患者115 例作为研究对象（IS 组），根据入院时国立卫生研究院卒中量表评分（national institute of health stroke scale，NIHSS）对患者进行神经功能损伤评分，将患者分为轻度组（NIHSS评分≤ 4 分，n=39）、中度组（4 分＜ NIHSS 评分≤ 20 分，n=35）和重度组（NIHSS 评分＞ 20 分，n=41），另选取同期在该院体检人员120 例作为对照组。采用实时荧光定量PCR（real time fluorescent quantitative PCR，qRT-PCR）法检测血清LncRNA SNHG8 表达水平；Spearman 相关性分析IS 患者血清LncRNA SNHG8 与NIHSS 评分的相关性；受试者工作特征（receiver operating characteristic curve，ROC）曲线分析血清LncRNA SNHG8 表达水平对IS 的诊断价值。结果 IS 组血清总胆固醇（total cholesterol，TC）（5.26±1.21 mmol/L）和低密度脂蛋白- 胆固醇（low-densitylipoprotein cholesterol，LDL-C）（2.23±0.53 mmol/L）水平高于对照组（4.35±1.43 mmol/L，1.51±0.65 mmol/L），差异具有统计学意义（t=5.255，9.284，均P ＜ 0.001）；血清高密度脂蛋白- 胆固醇（high-density lipoprotein cholesterol，HDL-C）（1.39±0.41 mmol/L）及LncRNA SNHG8（0.78±0.11）表达水平低于对照组（1.72±0.36 mmol/L，1.05±0.15），差异具有统计学意义（t=6.564，15.680，均P ＜ 0.001）；轻、中、重度组患者血清LncRNA SNHG8 表达水平分别为0.85±0.10，0.77±0.09 和0.71±0.08，三组间比较差异有统计学意义（F=24.173，P<0.001）；IS 患者血清LncRNASNHG8 与NIHSS 评分呈负相关性（r=-0.501，P<0.001）；血清LncRNA SNHG8 表达水平评估IS 患者的ROC 曲线下面积为0.873（95%CI：0.823 ～ 0.913），最佳截断值0.89，敏感度和特异度分别为85.22%，73.33%；IS 患者在入院24 h 内、治疗1 周后、治疗2 周后血清LncRNA SNHG8 表达水平为0.78±0.11，0.85±0.09 和0.93±0.08，表达水平依次升高，差异有统计学意义（F=73.064，P<0.001）。结论 LncRNA SNHG8 在IS 患者血清中表达水平降低，与患者病情严重程度有关，可能成为IS 诊断与病情评估的标志物。
Abstract:
Objective To investigate the expression level and clinical significance of long non-coding RNA small nucleolar RNA host gene 8 (LncRNA SNHG8) in the serum of patients with ischemic stroke (IS). Methods A total of 115 IS patients hospitalized in the Department of Neurology, the First Affiliated Hospital of Hebei North University from May 2019 to December 2021 were selected as the study subjects (IS group), according to the national institute of health stroke scale (NIHSS) score at admission, the patients’ neurological impairment was scored, the patients were divided into mild group (NIHSS score ≤ 4 points, n=39), moderate group (4 points20 points, n=41), another 120 cases of physical examination personnel in the hospital were taken as the control group. The expression level of serum LncRNA SNHG8 was detected by real time fluorescent quantitative PCR (qRT-PCR), the correlation between serum LncRNA SNHG8 and NIHSS scores in patients with IS was analyzed by spearman correlation, and the diagnostic value of serum LncRNA SNHG8 expression level in IS was analyzed by receiver operating characteristic curve (ROC curve). Results The levels of serum total cholesterol (TC) (5.26±1.21 mmol/L) and low-density lipoprotein cholesterol (LDL-C) (2.23±0.53 mmol/L) in IS group were higher than those in control group (4.35±1.43 mmol/L, 1.51±0.65 mmol/L), and the difference was statistically significant (t=5.255, 9.284, all P<0.001), the expression levels of serum high-density lipoprotein cholesterol (HDL-C) (1.39±0.41 mmol/L) and LncRNA SNHG8 (0.78±0.11) were lower than those of control group (1.72±0.36 mmol/L, 1.05±0.15), and the differences were statistically significant (t=6.564, 15.680, all P<0.001). The expression levels of serum LncRNA SNHG8 in the mild, moderate and severe groups were 0.85±0.10, 0.77±0.09 and 0.71±0.08, respectively, and there were significant differences among the three groups (F=24.173, P<0.001). Correlation analysis showed that serum LncRNA SNHG8 in IS patients was negatively correlated with NIHSS score (r=-0.501, P<0.001). The results of ROC analysis showed that the area under the ROC curve of serum LncRNA SNHG8 expression level in IS patients was 0.873 (95% CI: 0.823 ～ 0.913), the best cut-off value was 0.89, the sensitivity and the specificity were 85.22%, 73.33%, respectively. The expression levels of serum LncRNA SNHG8 in patients with IS within 24h after admission, 1 week after treatment, and 2 weeks after treatment were 0.78±0.11, 0.85±0.09 and 0.93±0.08, and the expression level increased sequentially, and the difference was statistically significant Significance (F=73.064, P<0.001). Conclusion The expression level of LncRNA SNHG8 IS decreased in IS patients, which was related to the severity of the disease. LncRNA SNHG8 may become a marker for diagnosis and disease evaluation of IS.

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备注/Memo

备注/Memo:
基金项目： 2022 年度河北省医学科学研究课题计划（20220029）：长链非编码RNA 与急性缺血性卒中疾病风险、严重性、炎症和预后的相关性。
作者简介：高继英（1982-）女，本科，主治医师，研究方向：高压氧专业，E-mail：rq7yuvo@163.com。
更新日期/Last Update: 2023-09-15