[1]张晓宇,张亚茹,张 莹,等.恶性黑色素瘤组织中circRNA_0020710 和circRNA_0079593 的水平表达及其临床价值[J].现代检验医学杂志,2023,38(05):90-92+179.[doi:10.3969/j.issn.1671-7414.2023.05.017]
　ZHANG Xiaoyu,ZHANG Yaru,ZHANG Ying,et al.Expression and Clinical Value of circRNA_0020710 and circrNA_0079593 in Malignant Melanoma Tissues[J].Journal of Modern Laboratory Medicine,2023,38(05):90-92+179.[doi:10.3969/j.issn.1671-7414.2023.05.017]
点击复制

恶性黑色素瘤组织中circRNA_0020710 和circRNA_0079593 的水平表达及其临床价值()
分享到：

《现代检验医学杂志》[ISSN:/CN:]

卷:
第38卷
期数:
2023年05期
页码:
90-92+179
栏目:
论著
出版日期:
2023-09-15

文章信息/Info

Title:
Expression and Clinical Value of circRNA_0020710 and circrNA_0079593 in Malignant Melanoma Tissues
文章编号:
1671-7414（2023）05-090-04
作者:
张晓宇; 张亚茹; 张 莹; 蒋志芳;  刘惠敏
（保定市第二中心医院检验科，河北涿州 072750）
Author(s):
ZHANG Xiaoyu; ZHANG Yaru; ZHANG Ying; JIANG Zhifang; LIU Huimin
（Department of Clinical Laboratory，the Second Central Hospital of Baoding，Hebei Zhuozhou 072750，China）
关键词:
恶性黑色素瘤; 环状核糖核酸_0020710; 环状核糖核酸_0079593
分类号:
R739.5；R730.43
DOI:
10.3969/j.issn.1671-7414.2023.05.017
文献标志码:
A
摘要:
目的 探讨环状核糖核酸（circular RNA, circRNA）_0020710 及环状核糖核酸（circular RNA, circRNA）_ 0079593 在恶性黑色素瘤组织中的表达及其临床价值。方法 选取2019 年1 月～ 2022 年3 月保定市第二中心医院收治的61 例恶性黑色素瘤患者，采用实时荧光定量PCR 法检测恶性黑色素瘤组织及正常组织circRNA_ 0020710 及circRNA_0079593 表达水平，并分析circRNA_0020710 及circRNA_0079593 表达水平与恶性黑色素瘤患者临床特征的关系。应用受试者工作特征（ROC）曲线分析circRNA_0020710 及circRNA_0079593 对恶性黑色素瘤诊断及其预测淋巴结转移的价值。结果 与正常组织相比，恶性黑色素瘤组织circRNA_0020710（4.95±2.37 vs 1.84±0.92）及circRNA_0079593（2.70±1.15 vs 0.91±0.34）表达水平均明显升高，差异具有统计学意义（t=18.946，15.248，均P<0.001）。恶性黑色素瘤患者circRNA_0020710 及circRNA_0079593 表达水平升高与组织分级Ⅲ～Ⅳ级、肿瘤部位、低分化及淋巴结转移有关（t=9.620 ～ 20.713，均P<0.001）。ROC 曲线显示，circRNA_0020710 及circRNA_0079593 诊断恶性黑色素瘤及预测淋巴结转移的曲线下面积分别为0.847（95%CI：0.787 ～ 0.905），0.806（95%CI：0.748 ～ 0.864），0.887（95%CI：0.829 ～ 0.948）和0.842（95%CI：0.793 ～ 0.907），其敏感度和特异度均较好。结论 circRNA_0020710 及circRNA_0079593 水平在恶性黑色素瘤组织中明显升高，与患者临床特征有关，可作为该病诊断及病情判断的分子标志物。
Abstract:
Objective To investigate the expression and clinical value of circular RNA（circRNA）_0020710 and circular RNA（circRNA）_0079593 in malignant melanoma tissues. Methods 61 patients with malignant melanoma admitted to the Second Central Hospital of Baoding from January 2019 to March 2022 were selected. The levels of circRNA_0020710 and circrNA_0079593 in malignant melanoma tissues and normal tissues were detected by real-time PCR. Analyze the relationship between the expression of circRNA_0020710 and circRNA_0079593 levels and the clinical characteristics of malignant melanoma patients. The ROC curve was used to analyze the value of circRNA_0020710 and circRNA_0079593 in the diagnosis and prediction of lymph node metastasis of malignant melanoma. Results The levels of circRNA_0020710（4.95±2.37 vs 1.84±0.92）and circRNA_0079593（2.70±1.15 vs 0.91±0.34）in malignant melanoma tissues were significantly higher than those in normal tissues (t=18.946，15.248，all P<0.001). The increased expression levels of circRNA_0020710 and circRNA_0079593 in malignant melanoma patients were associated with tissue grade Ⅲ～Ⅳ , tumor location, low differentiation and lymph node metastasis (t=9.620 ～ 20.713，all P <0.001). ROC curve showed that the areas under the curve of circRNA_0020710 and circRNA_0079593 for diagnosing malignant melanoma and predicting lymph node metastasis were 0.847 （95%CI：0.787 ～ 0.905），0.806（95%CI：0.748 ～ 0.864），0.887（95%CI：0.829 ～ 0.948）and 0.842（95%CI：0.793 ～ 0.907）, respectively, with good sensitivity and specificity. Conclusion The levels of circRNA_0020710 and circRNA_0079593 were significantly increased in malignant melanoma tissues, which were related to the clinical characteristics of patients. It can be used as molecular markers for the diagnosis and diagnosis of the disease.

参考文献/References:

[1] BROWN R V S, HILLESHEIM D, TOMASI Y T, et al. Mortality from malignant skin melanoma in elderly Brazilians: 2001 to 2016[J]. An Bras Dermatol, 2021, 96(1): 34-39.
[2] KHORSANDI K, ESFAHANI H, ABRAHAMSE H. Characteristics of circRNA and its approach as diagnostic tool in melanoma [J]. Expert Rev Mol Diagn, 2021, 21(10): 1079-1094.
[3] MECOZZI N, VERA O, KARRETH F A. Squaring the circle: circRNAs in melanoma [J]. Oncogene, 2021, 40(37): 5559-5566.
[4] WEI Chuanyuan, ZHU Mengxuan, LU Nanhang, et al. Circular RNA circ_0020710 drives tumor progression and immune evasion by regulating the miR-370-3p/CXCL12 axis in melanoma[J]. Molecular Cancer, 2020, 19(1): 84.
[5] ZHAO Feifei, JIA Zhifeng, FENG Yingfa, et al. Circular RNA circ_0079593 enhances malignant melanoma progression by the regulation of the miR-573/ABHD2 axis[J]. Journal of Dermatological Science, 2021, 102(1): 7-15.
[6] 《中国黑色素瘤规范化病理诊断专家共识2017 年版》编写组. 中国黑色素瘤规范化病理诊断专家共识（2017 年版）[J]. 中华病理学杂志,2018,47(1):7-13. Writing Group of Expert Consensus on Standardized Pathological Diagnosis of Melanoma China(2017 Edition). Expert consensus on standardized pathological diagnosis of melanoma China(2017 Edition) [J].Chinese Journal of Pathology, 2018, 47(1):7-13.
[7] 边鹊桥, 王军．肢端黑子国内首报[J]. 临床皮肤科杂志, 2018, 47(7): 430-432. BIAN Queqiao, WANG Jun. Acral lentigo: the first case reported in China[J]. Journal of Clinical Dermatology, 2018, 47(7): 430-432.
[8] CONG Lele, YANG Hongfeng, SUN Hongyan, et al. Role of circular RNAs in the pathogenesis of malignant melanoma[J]. Critical Reviews in Eukaryotic Gene Expression, 2022, 32(3): 21-30.
[9] LU Jiajing, LI Ying. Circ_0079593 facilitates proliferation, metastasis, glucose metabolism and inhibits apoptosis in melanoma by regulating the miR-516b/GRM3 axis[J]. Molecular and Cellular Biochemistry, 2020, 475(1/2): 227-237.
[10] KHORSANDI K, ESFAHANI H, ABRAHAMSE H. Characteristics of circRNA and its approach as diagnostic tool in melanoma [J]. Expert Rev Mol Diagn, 2021, 21(10):1079-1094.
[11] MONTICO B, GIURATO G, PECORARO G, et al. The pleiotropic roles of circular and long noncoding RNAs in cutaneous melanoma[J]. Molecular Oncology, 2022, 16(3): 565-593.
[12] HAO Y L, WANG X Q. Circ-0079593 promotes proliferation and migration of melanoma cells by sponging microRNA-433 and elevating EGFR expression [J]. European Review for Medical and Pharmacological Sciences, 2021, 25(2): 779-786.
[13] TANG Keyun, ZHANG Hanlin, LI Yaqi, et al. Circular RNA as a potential biomarker for melanoma: A systematic review [J]. Front Cell Dev Biol, 2021, 9:638548.

备注/Memo

备注/Memo:
作者简介：张晓宇（1982-），女，本科，主管检验师，主要从事临床皮肤病检验诊断研究，E-mail：zxyasxy521@163.com。
更新日期/Last Update: 2023-09-15