[1]孙 实a,李 想b.高血压并发冠状动脉粥样硬化性心脏病患者血清miR-27a-3p 和HOXC6 水平表达与冠状动脉病变的相关性研究[J].现代检验医学杂志,2024,39(03):67-71+182.[doi:10.3969/j.issn.1671-7414.2024.03.011]
　SUN Shia,LI Xiangb.Correlation between Serum miR-27a-3p and HOXC6 Levels and Coronary Artery Disease in Patients with Hypertension Complicated with Coronary Atherosclerotic Heart Disease[J].Journal of Modern Laboratory Medicine,2024,39(03):67-71+182.[doi:10.3969/j.issn.1671-7414.2024.03.011]
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高血压并发冠状动脉粥样硬化性心脏病患者血清miR-27a-3p 和HOXC6 水平表达与冠状动脉病变的相关性研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第39卷
期数:
2024年03期
页码:
67-71+182
栏目:
论著
出版日期:
2024-05-15

文章信息/Info

Title:
Correlation between Serum miR-27a-3p and HOXC6 Levels and Coronary Artery Disease in Patients with Hypertension Complicated with Coronary Atherosclerotic Heart Disease
文章编号:
1671-7414（2024）03-067-06
作者:
孙 实a; 李 想b
（北大荒集团总医院 a. 心内科；b. 病理科，哈尔滨 150088）
Author(s):
SUN Shia;  LI Xiangb
（a. Department of Cardiology; b. Department of Pathology, Beidahuang Group General Hospital, Harbin 150088, China）
关键词:
微小核糖核酸-27a-3p; 同源异型盒C6; 高血压; 冠状动脉粥样硬化性心脏病; 冠状动脉病变
分类号:
R541.3；R392.11
DOI:
10.3969/j.issn.1671-7414.2024.03.011
文献标志码:
A
摘要:
目的 探讨高血压并发冠状动脉粥样硬化性心脏病（冠心病）患者血清微小核糖核酸-27a-3p（miR-27a-3p）和同源异型盒C6（homeobox C6，HOXC6）水平表达与冠状动脉病变的相关性，为高血压并发冠心病的防治提供一定参考。方法 选取北大荒集团总医院2021 年6 月～ 2023 年6 月接诊的246 例高血压患者为研究对象，其中129 例高血压并发冠心病患者作为并发组，117 例单纯高血压患者作为未并发组。采用实时荧光定量PCR（quantitative real timePCR，qRT-PCR）检测血清miR-27a-3p 和HOXC6 表达水平；运用Spearman 法分析高血压并发冠心病患者血清miR-27a-3p 和HOXC6 水平与冠状动脉病变的相关性；多因素Logistic 回归分析高血压并发冠心病的影响因素；受试者工作特征（receiver operating characteristic，ROC）曲线分析血清miR-27a-3p，HOXC6 水平对高血压并发冠心病的预测价值。结果 并发组高血压病程（6.69±1.35 年）、总胆固醇（total cholesterol，TC）（5.96±0.86mmol/L）、低密度脂蛋白- 胆固醇（low-density lipoprotein cholesterol，LDL-C）（3.36±0.51mmol/L）均显著高于未并发组（5.87±1.32 年，5.42±0.67mmol/L，2.98±0.48mmol/L），并发组血清miR-27a-3p（1.35±0.34），HOXC6（1.29±0.33）水平均显著高于未并发组（1.06±0.23，1.03±0.25），差异具有统计学意义（t=4.808，5.454，6.001，7.755，6.911，均P ＜ 0.05）；轻度组、中度组、重度组患者血清miR-27a-3p（0.54 ± 0.13，1.19 ± 0.31，2.18 ± 0.55）和HOXC6（0.47 ± 0.12，1.12± 0.31，2.13 ± 0.52）表达水平逐渐升高，差异具有统计学意义（F=191.026，191.026，均P ＜ 0.05）。高血压并发冠心病患者血清miR-27a-3p，HOXC6 与SYNTAX 评分具呈正相关性（r=0.563，0.517，均P ＜ 0.05）；血清miR-27a-3p[OR(95%CI：1.627(1.177 ～ 2.248)]、HOXC6[OR(95%CI) ：1.715(1.069 ～ 2.750)] 是影响高血压并发冠心病的独立危险因素（P ＜ 0.05）；血清miR-27a-3p 和HOXC6 联合预测高血压并发冠心病的AUC 为0.896（95%CI：0.851 ～ 0.931），优于各自单独诊断（Z =2.133，2.815，均P ＜ 0.05）。结论 高血压并发冠心病患者血清miR-27a-3p，HOXC6 水平均显著升高，且与冠状动脉病变密切相关，二者联合对高血压并发冠心病具有较高的预测价值。
Abstract:
Objective To investigate the correlation between serum microRNA (miR)-27a-3p and homeobox C6 (HOXC6) levels expression and coronary artery disease in patients with hypertension complicated by coronary atherosclerotic heart disease (CHD), and provide some references for the prevention and treatment of hypertension complicated with coronary heart disease. Methods A total of 246 hypertensive patients accepted by Beidahuang Group General Hospital from June 2021 to June 2023 were collected as the study subjects, with 129 hypertensive patients complicated with coronary heart disease as the concurrent group and 117 simple hypertensive patients as the non concurrent group. Quantitative real time fluorescent PCR (qRT-PCR) was applied to detect the expression levels of miR-27a-3p and HOXC6 in serum. Spearman method was applied to analyze the correlation between serum miR-27a-3p and HOXC6 levels and coronary artery disease in hypertensive patients with coronary heart disease. Multivariate logistic regression was applied to analyze the influencing factors of hypertension complicated with coronary heart disease. Receiver operating characteristic (ROC) curve was applied to analyze the predictive value of serum miR- 27a-3p and HOXC6 levels for hypertension complicated with coronary heart disease. Results The course of hypertension(6.69±1.35 年), total cholesterol (TC)(5.96±0.86mmol/L) and low-density lipoprotein cholesterol (LDL-C) (3.36±0.51mmol/L) in the concurrent group were higher than those in the non concurrent group(5.87±1.32 年, 5.42±0.67mmol/L, 2.98±0.48mmol/L), the serum levels of miR-27a-3p(1.35±0.34) and HOXC6(1.29±0.33) in the concurrent group were higher than those in the non concurrent group(1.06±0.23, 1.03±0.25), and the differences were statistically significant (t=4.808, 5.454, 6.001, 7.755, 6.911, all P<0.05). The expression levels of miR-27a-3p (0.54 ± 0.13, 1.19 ± 0.31, 2.18 ± 0.55) and HOXC6 (0.47 ± 0.12, 1.12 ± 0.31, 2.13 ± 0.52) in mild, moderate and severe groups were gradually increased, and the differences were significant (F=191.026, 191.026, all P<0.05). There were positive correlations between serum miR-27a-3p, HOXC6, and SYNTAX scores in patients with hypertension complicated by coronary heart disease (r=0.563，0.517， all P<0.05). Serum miR-27a-3p [OR(95%CI)：1.627(1.177 ～ 2.248)] and HOXC6 [OR(95%CI)：1.715(1.069 ～ 2.750)] were independent risk factors for hypertension complicated with coronary heart disease (all P<0.05). The combination of miR-27a-3p and HOXC6 levels had an area under the curve (AUC) of 0.896 (95%CI:0.851 ～ 0.931) for predicting hypertension complicated with coronary heart disease, which was superior to their respective individual diagnoses (Z=2.133, 2.815, all P<0.05). Conclusion The serum levels of miR-27a-3p and HOXC6 in patients with hypertension complicated by coronary heart disease were increased, which was closely related to coronary artery disease. The combination of the two has a high predictive value for hypertension complicated with coronary heart disease.

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备注/Memo

备注/Memo:
作者简介：孙实（1988-），女，硕士研究生，主治医师，从事心内相关临床研究，E-mail： shin08462840@163.com。
通讯作者：李想（1988-），男，本科，主治医师，从事临床病理等相关研究，E-mail： 18645615046@163.com。
更新日期/Last Update: 2024-05-15