[1]王春燕,刘慧赏,马少未.妊娠期糖尿病患者血清LncRNA DANCR 和miR-33a-5p 水平表达对妊娠结局预测价值研究[J].现代检验医学杂志,2024,39(03):84-89.[doi:10.3969/j.issn.1671-7414.2024.03.014]
　WANG Chunyan,LIU Huishang,MA Shaowei.Study on the Predictive Value of Serum LncRNA DANCR and miR-33a-5p Levels in Patients with Gestational Diabetes Mellitus on Pregnancy Outcome[J].Journal of Modern Laboratory Medicine,2024,39(03):84-89.[doi:10.3969/j.issn.1671-7414.2024.03.014]
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妊娠期糖尿病患者血清LncRNA DANCR 和miR-33a-5p 水平表达对妊娠结局预测价值研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第39卷
期数:
2024年03期
页码:
84-89
栏目:
论著
出版日期:
2024-05-15

文章信息/Info

Title:
Study on the Predictive Value of Serum LncRNA DANCR and miR-33a-5p Levels in Patients with Gestational Diabetes Mellitus on Pregnancy Outcome
文章编号:
1671-7414（2024）03-084-06
作者:
王春燕; 刘慧赏; 马少未
（衡水市第四人民医院妇产科，河北衡水053000）
Author(s):
WANG Chunyan;  LIU Huishang;  MA Shaowei
(Department of Obstetrics and Gynecology, the Fourth People’s Hospital of Hengshui, Hebei Hengshui 053000，China)
关键词:
妊娠期糖尿病; 妊娠结局; 长链非编码核糖核酸分化拮抗非蛋白编码RNA; 微小RNA-33a-5p
分类号:
R714.256；R392.11
DOI:
10.3969/j.issn.1671-7414.2024.03.014
文献标志码:
A
摘要:
目的 探讨妊娠期糖尿病（gestational diabetes mellitus，GDM）患者血清长链非编码核糖核酸分化拮抗非蛋白编码RNA（long non coding RNA differentiation antagonizing non protein coding RNA，LncRNA DANCR）、微小RNA-33a-5p（miR-33a-5p）水平表达对妊娠结局的预测价值。方法 选取2021 年8 月～ 2023 年2 月于衡水市第四人民医院产检和分娩的154 例GDM 患者为GDM 组，并根据妊娠结局分为良好结局组（n=115）和不良结局组（n=39）；同期收集24 周葡萄糖耐量试验正常的149 例健康孕产妇作为对照组。采用实时荧光定量PCR(qRT-PCR) 检测血清LncRNADANCR 和miR-33a-5p 水平；采用受试者工作特征（receiver operating characteristic，ROC）曲线分析血清LncRNADANCR 和miR-33a-5p 预测GDM 患者不良妊娠结局的价值；采用Pearson 相关性分析GDM 不良妊娠结局患者血清LncRNA DANCR，miR-33a-5p 与空腹血糖（FBG）、空腹胰岛素（FINS）、稳态模式评估法计算胰岛素抵抗指数(HOMA-IR) 的相关性；Logistic 回归分析GDM 患者不良妊娠结局的影响因素。结果 GDM 组血清LncRNA DANCR水平（0.69 ± 0.15）显著低于对照组（1.01 ± 0.22），miR-33a-5p（1.59 ± 0.40）及不良妊娠结局总发生率（25.34%）显著高于对照组（1.02 ± 0.23，5.36%），差异具有统计学意义（t/χ2=14.835，15.140，23.011，均P ＜ 0.05）。不良妊娠结局组血清LncRNA DANCR 水平（0.50 ± 0.14）显著低于良好结局组（0.75 ± 0.18），miR-33a-5p（2.00 ± 0.58），FBG（8.97±0.66mmol/L），FINS（18.63±1.31pmol/ml）和HOMA IR（7.42±0.98）显著高于良好结局组(1.45 ± 0.26，8.01±0.59mmol/L，14.32±1.29pmol/ml，5.10±0.86)，差异具有统计学意义（t=7.895~17.961，均P ＜ 0.05）。LncRNADANCR，miR-33a-5p 单独及二者联合预测GDM患者不良妊娠结局的曲线下面积（area under curve，AUC）分别为0.820，0.819 和0.897。GDM 不良妊娠结局患者血清LncRNA DANCR 与FBG，FINS，HOMA-IR 呈负相关（r=-0.498，-0.513，-0.509，均P ＜ 0.05），miR-33a-5p 与FBG，FINS，HOMA-IR 呈正相关（r=0.517，0.494，0.507，均P ＜ 0.05）。LncRNA DANCR 是影响GDM 患者不良妊娠结局的保护因素（OR=0.804，95%CI：0.693 ～ 0.933，P=0.004），miR-33a-5p 是影响GDM 患者不良妊娠结局的危险因素（OR=2.747，95%CI：1.444 ～ 5.225，P=0.002）。结论 GDM 不良妊娠结局患者LncRNA DANCR 降低，miR-33a-5p 升高，二者均是不良妊娠结局的影响因素。
Abstract:
Objective To investigate predictive value of serum levels of long non coding RNA differentiation antagonizes nonprotein coding RNA (LncRNA DANCR) and microRNA (miR)-33a-5p in patients with gestational diabetes mellitus (GDM) on pregnancy outcome. Methods A total of 154 GDM patients who underwent prenatal examination and delivery in the Fourth People’s Hospital of Hengshui from August 2021 to February 2023 were collected as the GDM group, and these patients were grouped into a good outcome group of 115 cases and an adverse outcome group of 39 cases based on pregnancy outcomes. Meantime, 149 healthy pregnant women with normal glucose tolerance test for 24 weeks were collected as the control group. Real time fluorescence quantitative PCR(qRT-PCR) method was applied to detect serum LncRNA DANCR and miR-33a-5p levels, and receiver operating characteristic (ROC) curve was used to analyze the value of serum LncRNA DANCR and miR- 33a-5p in predicting adverse pregnancy outcomes in GDM patients. Pearson correlation was applied to analyze the correlation among serum LncRNA DANCR，miR-33a-5p, fasting blood glucos(FBG）, fasting insulin（FINS）, and homeostasis model assessment of insulin resistance (HOMA-IR) in patients with GDM adverse pregnancy outcomes. Logistic regression was used to analyze the influencing factors of adverse pregnancy outcomes in GDM patients. Results The serum LncRNA DANCR level (0.69 ± 0.15) in the GDM group was lower than that in the control group(1.01 ± 0.22), while the level of miR-33a-5p (1.59 ± 0.40) and the total incidence of adverse pregnancy outcomes（25.34%） were higher than those in the control group（1.02 ± 0.23， 5.36%）, with significant differences (t/χ2=14.835，15.140，23.011，all P<0.05). The serum LncRNA DANCR level(0.50 ± 0.14) in the adverse outcome group was lower than that in the good outcome group(0.75 ± 0.18), while miR-33a-5p (2.00 ± 0.58), FBG（8.97±0.66mmol/L）, FINS（18.63±1.31pmol/ml） and HOMA-IR（7.42±0.98） were higher than those in the good outcome group(1.45 ± 0.26，8.01±0.59mmol/L，14.32±1.29pmol/ml，5.10±0.86), the differences were statistically significant(t=7.895 ～ 17.961, all P<0.05). The areas under curve (AUC) of predicting adverse pregnancy outcomes in GDM patients with LncRNA DANCR and miR-33a-5p alone and in combination were 0.820, 0.819 and 0.897, respectively. For patients with GDM adverse pregnancy outcomes, serum LncRNA DANCR was negatively correlated with FBG, FINS and HOMA IR (r=-0.498，-0.513，-0.509, all P<0.05), while miR-33a-5p was positively correlated with FBG, FINS and HOMA-IR (r=0.517, 0.494, 0.507, all P<0.05). LncRNA DANCR was a protective factor that affected adverse pregnancy outcomes in GDM patients (OR=0.804，95%CI: 0.693 ～ 0.933, P=0.004)；while miR-33a-5p was a risk factor for adverse pregnancy outcomes in GDM patients(OR=2.747，95%CI: 1.444 ～ 5.225, P=0.002). Conclusion LncRNA DANCR was decreased and miR-33a-5p was increased in GDM patients with adverse pregnancy outcomes, indicating both may be influencing factors for adverse pregnancy outcomes.

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备注/Memo

备注/Memo:
基金项目：衡水市科技计划项目（2022014021Z）：妊娠期糖尿病危险因素及其预防价值的分析。
作者简介：王春燕（1980-），女，本科，主治医师，研究方向：妊娠期糖尿病，E-mail：nkg99t9@163.com。
更新日期/Last Update: 2024-05-15