[1]李青松,赵聪平,刘 静,等.CRISPR-Cas13a 系统在病原体检测应用中的最新研究进展[J].现代检验医学杂志,2024,39(03):199-204.[doi:10.3969/j.issn.1671-7414.2024.03.034]
　LI Qingsong,ZHAO Congping,LIU Jing,et al.Recent Advances in the Application of CRISPR-Cas13a Systems in Pathogen Detection[J].Journal of Modern Laboratory Medicine,2024,39(03):199-204.[doi:10.3969/j.issn.1671-7414.2024.03.034]
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CRISPR-Cas13a 系统在病原体检测应用中的最新研究进展()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第39卷
期数:
2024年03期
页码:
199-204
栏目:
综述
出版日期:
2024-05-15

文章信息/Info

Title:
Recent Advances in the Application of CRISPR-Cas13a Systems in Pathogen Detection
文章编号:
1671-7414（2024）03-199-06
作者:
李青松1; 赵聪平1; 刘 静1; 杨 均2; 燕思羽1; 张银河3
（1. 西南医科大学公共卫生学院，四川泸州646000；2. 泸州市人民医院，四川泸州 646000；3. 太极集团重庆涪陵制药厂有限责任公司，重庆 408000）
Author(s):
LI Qingsong1; ZHAO Congping1; LIU Jing1; YANG Jun2; YAN Siyu1; ZHANG Yinhe3
（1. School of Public Health，Southwest Medical University, Sichuan Luzhou 646000, China; 2. Luzhou People’s Hospital, Sichuan Luzhou 646000, China; 3. Taiji Group Chongqing Fuling Pharmaceutical Co. Ltd., Chongqing 408000, China）
关键词:
规律成簇的间隔短回文重复序列及相关蛋白13a 系统; 病原体; 核酸检测
分类号:
R446；
DOI:
10.3969/j.issn.1671-7414.2024.03.034
文献标志码:
A
摘要:
规律成簇的间隔短回文重复序列及相关蛋白（CRISPR-Cas）系统已作为近年来兴起的新一代分子诊断工具，被广泛应用于基因编辑、核酸检测等领域，其中Cas13a 亚型因其在核酸检测中高特异度、高灵敏度、无需昂贵设备、操作简单、费用低廉等特点，在病原体检测领域展现出巨大的潜力。该文综述了CRISPR-Cas13a 的作用机制、在病原体检测中的应用以及相关检测方法，并对Cas13a 应用前景进行了展望。以期为CRISPR-Cas13a 系统的进一步研究及其在病原体检测中的应用提供借鉴和参考。
Abstract:
As a new generation of molecular diagnostic tools emerged in recent years, the clustered regularly interspaced short palindromic repeats/CRISPR-associated (CRISPR-Cas) systems have been widely used in gene editing, nucleic acid detection, and other fields. Due to its high specificity, high sensitivity, without expensive equipment, simple operation, and inexpensive characteristics in nucleic acid detection, the Cas13a subtype has shown great potential in the field of pathogen detection. This article reviews the mechanism of action of CRISPR-Cas13a, its application in pathogen detection, and related detection methods, and looks forward to the application prospects of Cas13a. To facilitate future investigations on the CRISPR-Cas13a systems and their potential in pathogen detection, this study aims to offer inspiration and serve as a valuable reference.

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备注/Memo

备注/Memo:
基金项目：西南医科大学应用基础项目（NO. 2021ZKQN002）。
作者简介：李青松（2001-），男，本科在读，主要研究方向：病原体检测，E-mail: 17380784728@163.com。
通讯作者：刘静，博士，讲师，主要从事荧光传感检测的研究，E-mail: liujing.1583@163.com。
更新日期/Last Update: 2024-05-15