[1]梅 竹,任 赛,唐思恬,等.膀胱癌患者尿液外泌体中miR-494-3p，LncRNA MAGI2-AS3 表达水平及临床价值研究[J].现代检验医学杂志,2024,39(04):5-9+55.[doi:10.3969/j.issn.1671-7414.2024.04.002]
　MEI Zhu,REN Sai,TANG Sitian,et al.Study on Expression Levels and Clinical Value of miR-494-3p and LncRNA MAGI2-AS3 in Urine Exosomes of Patients with Bladder Cancer[J].Journal of Modern Laboratory Medicine,2024,39(04):5-9+55.[doi:10.3969/j.issn.1671-7414.2024.04.002]
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膀胱癌患者尿液外泌体中miR-494-3p，LncRNA MAGI2-AS3 表达水平及临床价值研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第39卷
期数:
2024年04期
页码:
5-9+55
栏目:
论著
出版日期:
2024-07-15

文章信息/Info

Title:
Study on Expression Levels and Clinical Value of miR-494-3p and LncRNA MAGI2-AS3 in Urine Exosomes of Patients with Bladder Cancer
文章编号:
1671-7414（2024）04-005-06
作者:
梅 竹; 任 赛; 唐思恬; 胡礼仪
（重庆两江新区人民医院医学检验科，重庆 401121）
Author(s):
MEI Zhu;  REN Sai;  TANG Sitian;  HU Liyi
(Department of Medical Laboratory; Chongqing Liangjiang New Area People’s Hospital, Chongqing 401121, China)
关键词:
膀胱癌; 微小RNA-494-3p; 长链非编码RNA MAGI2- 反义RNA 3; 外泌体
分类号:
R737.14；R730.43
DOI:
10.3969/j.issn.1671-7414.2024.04.002
文献标志码:
A
摘要:
目的 探讨膀胱癌患者尿液外泌体中微小核糖核酸（microRNA，miR）-494-3p，长链非编码RNA（long noncodingRNA，LncRNA）MAGI2- 反义RNA 3（MAGI2-antisense RNA 3，MAGI2-AS3）表达水平及临床应用价值。方法 选取重庆两江新区第一人民医院2021 年7 月~2023 年6 月收治的105 例膀胱癌患者为癌变组，另选取同期膀胱良性疾病患者101 例作为良性组。实时荧光定量PCR（qRT-PCR）检测尿液外泌体miR-494-3p 和LncRNA MAGI2-AS3水平。Kappa 检验分析尿液外泌体miR-494-3p 和LncRNA MAGI2-AS3 与病理活检诊断膀胱癌的一致性；受试者工作特征（receiver operating characteristic，ROC）曲线分析尿液外泌体miR-494-3p 和LncRNA MAGI2-AS3 水平对膀胱癌的诊断价值。结果 癌变组尿液外泌体miR-494-3p 水平（1.41±0.32）高于良性组（1.02±0.24），LncRNA MAGI2-AS3 水平（0.79±0.19）低于良性组（1.05±0.22），差异具有统计学意义（t=9.866，9.089，均P ＜ 0.05）。miR-494-3p 高表达组肿瘤直径＞ 3 cm，T3~T4 期以及淋巴结转移的占比显著高于miR-494-3p 低表达组，差异具有统计学意义（χ2=5.806，6.999，8.289，均P ＜ 0.05）。LncRNA MAGI2-AS3 低表达组T3~T4 期及发生淋巴结转移的比例明显高于LncRNA MAGI2-AS3 高表达组，差异具有统计学意义（χ2=9.244，7.795，均P ＜ 0.05）。尿液外泌体miR-494-3p和LncRNA MAGI2-AS3 联合与病理活检诊断膀胱癌的一致性较高（Kappa 值=0.718，P ＜ 0.05）。ROC 曲线显示，尿液外泌体miR-494-3p 和LncRNA MAGI2-AS3 水平单独诊断膀胱癌的AUC（95%CI）分别为0.812（0.752~0.863）和0.779（0.716~0.833），尿液外泌体miR-494-3p 和LncRNA MAGI2-AS3 水平联合诊断膀胱癌的AUC(95%CI)[0.877，（0.824~0.918）] 显著高于两者分别单独诊断（Z=2.053，2.647，P=0.040，0.008）。结论 膀胱癌患者尿液外泌体中miR-494-3p 水平升高，LncRNA MAGI2-AS3 水平下降，二者联合对膀胱癌的诊断价值较高。
Abstract:
Objective To investigate expression level and clinical application value of microRNA(miR)-494-3p and long noncoding RNA (LncRNA) MAGI2-antisense RNA 3 (MAGI2-AS3) in urine exosomes of patients with bladder cancer. Methods A total of 105 bladder cancer patients admitted to Chongqing Liangjiang New Area People’s Hospital from July 2021 to June 2023 were selected as the cancer group, and 101 patients with benign bladder diseases were as the benign group. Real-time fluorescence quantitative PCR(qRT-PCR) was applied to detect miR-494-3p and LncRNA MAGI2-AS3 levels in urine exosomes. Kappa test was used to analyze the consistency among miR-494-3p, LncRNA MAGI2-AS3 in urinary exosomes and pathological biopsy diagnosis of bladder cancer. Receiver operating characteristic (ROC) curve was applied to analyze the diagnostic value of miR-494-3p and LncRNA MAGI2-AS3 levels in urinary exosomes for bladder cancer. Results The level of miR-494-3p (1.41±0.32) in the urine exosomes of the cancer group was higher than that of the benign group(1.02±0.24), while the level of LncRNA MAGI2-AS3 [(0.79±0.19) vs (1.05±0.22)] was lower than that of the benign group(1.05±0.22), and the differences were statistically significant(t=9.866, 9.089, all P<0.05). The proportions of tumor diameter > 3 cm, T3~T4 stage, and lymph node metastasis in the high expression group of miR-494-3p were higher than those in the low expression group, and the differences were significant (χ2=5.806, 6.999, 8.289, all P<0.05). The proportions of T3~T4 stages and lymph node metastasis in the low expression group of LncRNA MAGI2-AS3 were higher than those in the high expression group, and the differences were significant (χ2=9.244, 7.795, all P<0.05). MiR-494-3p and LncRNA MAGI2-AS3 in urine exosomes combined with pathological biopsy showed high consistency in the diagnosis of bladder cancer (Kappa value=0.718, P<0.05). ROC curve showed that the areas under the curve(95% confidence interval) [AUC(95%CI)] of miR-494-3p and LncRNA MAGI2-AS3 levels in urinary exosomes alone for diagnosis of bladder cancer were 0.812 (0.752~0.863) and 0.779 (0.716~0.833), respectively. MiR-494-3p and LncRNA MAGI2-AS3 levels in urinary exosomes combined to diagnose the AUC (95%CI)of bladder cancer 0.877(0.824~0.918) were higher than that of AUC(95%CI) diagnosed separately (Z=2.053, 2.647, P=0.040, 0.008). Conclusion The level of miR-494-3p in urine exosomes of patients with bladder cancer was increased, while the level of LncRNA MAGI2-AS3 was decreased. The combination of the two may have high diagnostic value for bladder cancer.

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备注/Memo

备注/Memo:
基金项目：重庆市科卫联合医学科研项目（2022QNXM036）。
作者简介：梅竹（1984-），女，本科，主管检验师，研究方向：医学检验，E-mail：aecna3qq@163.com。
通讯作者：胡礼仪（1972-），男，硕士研究生，主任技师，研究方向：医学检验，E-mail：cqljhly@163.com。
更新日期/Last Update: 2024-07-15