[1]胥康妮,余 艳,张 琱,等.HPV阳性宫颈癌组织中AIB1 mRNA和ANCO1 mRNA表达及临床意义[J].现代检验医学杂志,2024,39(06):113-118.[doi:10.3969/j.issn.1671-7414.2024.06.019]
 XU Kangni,YU Yan,ZHANG Diao,et al.Expression and Clinical Significance of AIB1 mRNA and ANCO1 mRNA in HPV Positive Cervical Cancer Tissue[J].Journal of Modern Laboratory Medicine,2024,39(06):113-118.[doi:10.3969/j.issn.1671-7414.2024.06.019]
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HPV阳性宫颈癌组织中AIB1 mRNA和ANCO1 mRNA表达及临床意义()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第39卷
期数:
2024年06期
页码:
113-118
栏目:
论著
出版日期:
2024-11-15

文章信息/Info

Title:
Expression and Clinical Significance of AIB1 mRNA and ANCO1 mRNA in HPV Positive Cervical Cancer Tissue
文章编号:
1671-7414(2024)06-113-06
作者:
胥康妮余 艳张 琱王 珂程琪惠
(绵阳市第三人民医院妇产科,四川绵阳 621000)
Author(s):
XU Kangni YU Yan ZHANG Diao WANG Ke CHENG Qihui
(Deprtment of Obstetrics and Gynaecology, the Third People’s Hospital of Mianyang, Sichuan Mianyang 621000, China)
关键词:
宫颈癌人乳头状瘤病毒乳腺癌扩增基因1锚蛋白重复结构域11
分类号:
R737.33;R730.43
DOI:
10.3969/j.issn.1671-7414.2024.06.019
文献标志码:
A
摘要:
目的 研究人乳头状瘤病毒(HPV)阳性宫颈癌组织中乳腺癌扩增基因1(AIB1)、锚蛋白重复结构域11(ANKRD11/ANCO1)表达与临床病理特征的关系及对术后复发的预测价值。方法 选取2018 年1 月~ 2020 年1 月就诊于绵阳市第三人民医院的94 例HPV 阳性宫颈癌患者癌组织和癌旁组织,以同期50 例HPV 阴性宫颈癌组织为对照。采用实时荧光定量PCR(RT qPCR)检测HPV 阳性宫颈癌癌组织和癌旁组织、HPV 阴性宫颈癌组织中AIB1 mRNA,ANCO1 mRNA 表达。采用Pearson 分析HPV 阳性宫颈癌癌组织中AIB1 mRNA 与ANCO1 mRNA 表达的相关性。采用Logistic 回归分析筛选术后复发的影响因素。采用受试者工作特征(ROC)曲线研究AIB1 mRNA,ANCO1 mRNA 对患者术后复发的预测价值。结果 HPV 阳性宫颈癌癌组织中AIB1 mRNA(3.04±0.37)高于癌旁组织(0.87±0.21) 及HPV 阴性宫颈癌癌组织(1.02±0.33),ANCO1 mRNA(1.13±0.26)低于癌旁组织(1.91±0.35) 及HPV 阴性宫颈癌癌组织(1.82±0.36),差异具有统计学意义(t=68.499,53.137;23.649,17.434,均P < 0.05)。HPV 阳性宫颈癌癌组织中AIB1 mRNA 与ANCO1 mRNA 表达呈负相关(r= - 0.714,P < 0.001)。相比于FIGO 分期Ⅰ A ~Ⅰ B 期、无淋巴结转移患者,肿瘤FIGO 分期Ⅱ A 期、淋巴结转移HPV 阳性宫颈癌患者癌组织中AIB1 mRNA(3.88±0.32 vs 2.04±0.41,4.46±0.33 vs 2.16±0.46)较高,ANCO1 mRNA(0.67±0.29 vs 1.68±0.20,0.49±0.24 vs 1.53±0.32)较低,差异具有统计学意义(t=24.425,26.097;19.288,16.777,均P < 0.001)。FIGO 分期Ⅱ A 期、淋巴结转移、AIB1 mRNA 高水平[OR(95% CI):1.644(1.223 ~ 2.210);1.779(1.295 ~ 2.444),1.247(1.050 ~ 1.728)] 是影响HPV 阳性宫颈癌患者术后复发的危险因素,ANCO1 mRNA 高水平[OR(95% CI):0.634(0.451 ~ 0.891)] 是保护因素。AIB1mRNA,ANCO1 mRNA 联合检测对HPV 阳性宫颈癌患者术后复发预测AUC 下面积(95%CI)为0.914(0.863 ~ 0.952),高于单指标检测[0.821(0.782 ~ 0.869),0.794(0.763 ~ 0.847)],差异具有统计学意义(Z=4.123,4.432,均P < 0.001)。结论 HPV 阳性宫颈癌中AIB1 mRNA 升高,ANCO1 mRNA 降低,均参与HPV 阳性宫颈癌的发生发展,两者联合对评估术后复发具有较高的预测价值。
Abstract:
Objective To study the expression of amplified in breast cancer 1 (AIB1) and ankyrin repeat domain containing11 (ANKRD11/ANCO1) in human papillomavirus (HPV) positive cervical cancer and their relationship with clinicopathological characteristics and their predictive value for postoperative recurrence. Methods Cancer tissues and paracancer tissues of 94 HPV positive cervical cancer patients who visited the Third People’s Hospital of Mianyang from January 2018 to January 2020 were selected, while 50 HPV negative cervical cancer tissues during this period were taken as controls. Real-time qPCR (RT-qPCR) was conducted to detect AIB1 mRNA and ANCO1 mRNA expression in cancer tissue and paracancer tissues of HPV positive cervical cancer and cancer tissue of HPV negative cervical. Pearson analysis of the correlation between AIB1 mRNA and ANCO1 mRNA expression in HPV positive cervical cancer tissues. Logistic regression analysis was used to identify the risk factors for postoperative recurrence of HPVpositive cervical cancer patients. The predictive value of AIB1 mRNA, ANCO1 mRNA, and their combination in predicting postoperative recurrence in HPV positive cervical cancer patients was analyzed by receiver operating characteristic curve. Results Compared to the adjacent tissues of HPV positive cervical cancer and cancer tissues of HPV negative cervical cancer, AIB1 mRNA (3.04 ± 0.37 vs 0.87 ± 0.21, 1.02 ± 0.33) in HPV positive cervical cancer tissues was higher, while ANCO1 mRNA (1.13 ± 0.26 vs 1.91 ± 0.35, 1.82 ± 0.36) was lower, with significant differences (t=68.499, 53.137; 23.649, 17.434, all P<0.05). The expression of AIB1 mRNA and ANCO1 mRNA in HPV positive cervical cancer tissues showed a negative correlation (r= -0.714, P<0.001). Compared to patients with FIGO stage Ⅱ A ~Ⅱ B and without lymph node metastasis, AIB1 mRNA (3.88 ± 0.32 vs 2.04 ± 0.41, 4.46 ± 0.33 vs 2.16 ± 0.46) in HPV positive cervical cancer tissues with FIGO stage IIA and lymph node metastasis was higher, while ANCO1 mRNA (0.67 ± 0.29 vs 1.68 ± 0.20, 0.49 ± 0.24 vs 1.53 ± 0.32) was lower, with significant differences (t=24.425, 26.097;19.288, 16.777, all P<0.001). FIGO stage IIA, lymph node metastasis, and high AIB1 mRNA [OR(95%CI)=1.644 (1.223 ~ 2.210),1.779(1.295 ~ 2.444),1.247(1.050 ~ 1.728)] were risk factors for postoperative recurrence in HPV positive cervical cancer patients, while high ANCO1 mRNA [(OR (95%CI): 0.634(0.451 ~ 0.891)]was a protective factor. The AUC (95%CI) of AIB1 mRNA and ANCO11 mRNA combined for predicting postoperative recurrence in HPV positive cervical cancer patients was 0.914 (0.863 ~ 0.952), which was higher than the single indicator detection of 0.821 (0.782 ~ 0.869) and 0.794 (0.763 ~ 0.847), and the differences were significant (Z=4.123, 4.432, all P < 0.001). Conclusion The expression of AIB1 mRNA is increased and the expression of ANCO1 mRNA is reduced in HPV positive cervical cancer tissue, which are related to the occurrence and progression of cancer. The combination of the two has a high predictive value for evaluating postoperative recurrence.

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备注/Memo

备注/Memo:
基金项目:四川省医学科研课题(编号:S20077)。
作者简介:胥康妮(1984-),女,硕士,主治医师,主要从事妇科肿瘤方向的研究,E -mail:xukangni198405@163.com。
通讯作者:余艳(1983-),女,汉族,主要从事妇科肿瘤方面的研究,E- mail:2262784516@qq.com。
更新日期/Last Update: 2024-11-15