[1]许兆杰a,吴京学b,王 娇b,等.大连地区健康成年人群外周血T淋巴细胞15种免疫表型分析研究[J].现代检验医学杂志,2025,40(01):1-6+12.[doi:10.3969/j.issn.1671-7414.2025.01.001]
　XU Zhaojiea,WU Jingxueb,WANG Jiaob,et al.Analysis of 15 Immunophenotypes of Peripheral Blood T Lymphocytes in Dalian Area[J].Journal of Modern Laboratory Medicine,2025,40(01):1-6+12.[doi:10.3969/j.issn.1671-7414.2025.01.001]
点击复制

大连地区健康成年人群外周血T淋巴细胞15种免疫表型分析研究()
分享到：

《现代检验医学杂志》[ISSN:/CN:]

卷:
第40卷
期数:
2025年01期
页码:
1-6+12
栏目:
论著
出版日期:
2025-01-15

文章信息/Info

Title:
Analysis of 15 Immunophenotypes of Peripheral Blood T Lymphocytes in Dalian Area
文章编号:
1671-7414（2025）01-001-07
作者:
许兆杰a; 吴京学b; 王 娇b; 田 瑶b; 汤亚微b; 朱 杰b
（大连医科大学附属第二医院a. 检验科；b. 流式细胞中心，辽宁大连 116023）
Author(s):
XU Zhaojiea; WU Jingxueb; WANG Jiaob; TIAN Yaob; TANG Yaweib; ZHU Jieb
（a. Department of Clinical Laboratory；b. Department of Flow Cytometry Center，the Second Hospital of Dalian Medical University，Liaoning Dalian 116023，China)
关键词:
T 淋巴细胞; 免疫表型; 流式细胞术; 大连地区
分类号:
R446.6
DOI:
10.3969/j.issn.1671-7414.2025.01.001
文献标志码:
A
摘要:
目的 分析大连地区健康成年人外周血T 淋巴细胞15 种免疫表型特征与年龄及性别之间的关系。方法 选取2022 年10 月～ 2023 年6 月在大连医科大学附属第二医院体检中心诊疗的277 例健康成年人作为研究对象，其中男性154 例，女性123 例。按不同年龄将研究对象分为三组：青年组（18~44 岁，n=103）、中年组（45~60 岁，n=114）和老年组（>60 岁，n=60）。通过流式细胞术检测外周血T 淋巴细胞免疫表型，包括初始（N）、中央记忆（CM）、效应记忆（EM）、终末分化（TEM）、活化（HLA-DR+）及衰老（CD28-）CD4+ 和CD8+ T 淋巴细胞绝对计数和百分比，比较不同性别和不同年龄段T 淋巴细胞免疫表型绝对计数和百分比的差异性；同时采用Spearman 相关性分析评估年龄与T 淋巴细胞免疫表型绝对计数和百分比的相关性。结果 与青年组比较，中年组和老年组中CD8+TEM，CD4+HLADR+，CD8+HLA-DR+ 和CD8+CD28- T 淋巴细胞绝对计数和百分比升高（Z=2.009 ～ 6.607），CD8+N T 淋巴细胞绝对计数和百分比降低（Z=5.574 ～ 7.999），差异具有统计学意义（均P<0.05）。与女性组比较，男性组中CD8+CM，CD8+HLA-DR+，CD8+CD28- T 淋巴细胞绝对计数和百分比升高（Z=2.945 ～ 6.131），CD3+，CD4+ 和CD8+N T 淋巴细胞绝对计数和百分比降低（Z=2.075 ～ 4.225），差异具有统计学意义（均P<0.05）。Spearman 相关性分析表明，年龄与CD4+CM，CD4+HLA-DR+，CD4+CD28-，CD8+TEM，CD8+HLA-DR+ 和CD8+CD28- T 淋巴细胞绝对计数和百分比呈正相关（r=0.125 ～ 0.479，均P<0.05），与CD4+N 和CD8+N T 淋巴细胞绝对计数和百分比呈负相关（r=-0.538 ～ -0.148，均P<0.05）。结论 大连地区健康成年人外周血T 淋巴细胞15 种免疫表型受年龄和性别影响，有必要建立适宜当地的参考区间，为机体免疫功能评估提供更精准的参考依据。
Abstract:
Objective To analyze the relationship between 15 immunophenotypes of peripheral blood T cells and age and gender in healthy adults in the Dalian area. Methods A total of 277 healthy adults admitted to the physical examination center of the Second Affiliated Hospital of Dalian Medical University from October 2022 to June 2023 were selected as the research subjects, including 154 males and 123 females. They were divided into three groups according to age: young group（18 ～ 44 years，n =103）, middle-aged group（45 ～ 60 years，n=114） and old age group（>60 years，n=60）. Flow cytometry was used to determine immunophenotypes of T cells, including the absolute count and proportion of na?ve cells (N), central memory (CM), effector memory (EM), terminal effector memory (TEM), activation (HLA-DR+) and senescence (CD28-) CD4+ and CD8+ T cells, and the differences of them among age and gender were analyzed. Spearman correlation analysis was also used to evaluate the correlation between age and immunophenotypes of T cells. Results Compared with the young group, the absolute count and proportion of CD8+TEM, CD4+HLA-DR+, CD8+HLA-DR+ and CD8+CD28- T lymphoaytes cells were increased in the middleaged group and old age group (Z=2.009 ～ 6.607), while the absolute count and proportion of CD8+N T cells were decreased in the middle-aged group (Z=5.574 ～ 7.999) and old age group, and the differences were statistically significant (all P<0.05),respectively. Compared with the female group, the absolute count and proportion of CD8+CM, CD8+HLA-DR+and CD8+CD28-T cells were increased in the female group (Z=2.945 ～ 6.131), while the absolute count and proportion of CD3+, CD4+ and CD8+N T cells were decreased in the male group (Z=2.075 ～ 4.225), and the differences were statistically significant(all P<0.05), respectively. Spearman correlation analysis showed that age was positively correlated with the absolute count and proportion of CD4+CM, CD4+HLA-DR+, CD4+CD28-, CD8+TEM, CD8+HLA-DR+ and CD8+CD28- T lymphocytes cells (r=0.125 ～ 0.479, all P<0.05), while negatively correlated the absolute count and proportion of CD4+N and CD8+N T lymphocytes cells (r=-0.538 ～ - 0.148, all P<0.05). Conclusion The 15 immunophenotypes of peripheral blood T lymphocytes cells in healthy adults from Dalian area are affected by age and gender, so it is necessary to establish a suitable local reference interval to provide a more accurate reference for immune function assessment.

参考文献/References:

[1] WALDMAN A D, FRITZ J M, LENARDO M J. A guide to cancer immunotherapy: from T cell basic science to clinical practice [J]. Nature Reviews Immunology, 2020, 20(11): 651-668.
[2] BARBERIO M T, PAWLOWSKA N, DHAWAN M, et al. Exhausted T cell signature predicts immunotherapy response in ER-positive breast cancer[J]. Nature Communications, 2020, 11(1): 3584.
[3] BHANDARKAR A R, BHANDARKAR S, VUKSANOVIC D B, et al. Characterizing T-cell dysfunction and exclusion signatures in malignant peripheral nerve sheath tumors reveals susceptibilities to immunotherapy[J]. Journal of Neurooncology, 2023, 164(3): 693-699.
[4] MIRSHARIF E S, CHENARY M R, BOZORGMEHR M, et al. Immunophenotyping characteristics of COVID-19 patients: Peripheral blood CD8+ HLA-DR+ T cells as a biomarker for mortality outcome[J]. Journal of Medical Virology, 2023, 95(1): e28192.
[5] DU Juan, WEI Lirong, LI Guoli, et al. Persistent high percentage of HLA-DR+CD38high CD8+ T cells associated with immune disorder and disease severity of COVID-19[J]. Frontiers in Immunology, 2021, 12: 735125.
[6] GORONZY J J, HU Bin, KIM C, et al. Epigenetics of T cell aging[J].Journal of Leukocyte Biology, 2018, 104(4): 691-699.
[7] BISSET L R, LUNG T L, KAELIN M, et al. Reference values for peripheral blood lymphocyte phenotypes applicable to the healthy adult population in Switzerland[J]. European Journal of Haematology, 2004, 72(3): 203-212.
[8] RANE S, HOGAN T, SEDDON B, et al. Age is not just a number: Naive T cells increase their ability to persist in the circulation over time[J]. PLoS Biology, 2018, 16(4): e2003949.
[9] XIA Ying, LIU Aqing, LI Wentao, et al. Reference range of na?ve T and T memory lymphocyte subsets in peripheral blood of healthy adult[J]. Clinical and Experimental Immunology, 2022, 207(2): 208-217.
[10] G?MEZ R O, ARQUEROS C, GALANO C, et al. Effector mechanisms of CD8+ HLA-DR+ T cells in breast cancer patients who respond to neoadjuvant chemotherapy[J]. Cancers, 2021, 13(24): 6167.
[11] 林帝金, 刘纯岳, 方俊粤, 等. 乳腺肿瘤患者外周血中T 淋巴细胞表面活化分子和Tregs 的表达研究[J].现代检验医学杂志, 2018, 33(5):31-34，37. LIN Dijin, LIU Chunyue, FANG Junyue, et al. Expression of T lymphocyte surface activating molecules and tregs in peripheral blood of patients with breast neoplass [J]. Journal of Modern Laboratory Medicine, 2018, 33(5): 31-34，37.
[12] JIN Jun, MU Yunmei, ZHANG Huimin, et al. CISH impairs lysosomal function in activated T cells resulting in mitochondrial DNA release and inflammaging[J]. Nature Aging, 2023, 3(5): 600-616.
[13] WANG Yun, LUO Ying, TANG Guoxing, et al. HLA-DR expression level in CD8+ T cells correlates with the severity of children with acute infectious mononucleosis[J].Frontiers in Immunology, 2021, 12: 753290.
[14] WENG Nanping, AKBAR A N, GORONZY J. CD28- T cells: their role in the age-associated decline of immune function[J]. Trends in Immunology, 2009, 30(7): 306-312.
[15] LI Mingde, YAO Danlin, ZENG Xiangbo, et al. Age related human T cell subset evolution and senescence[J].Immunity & Ageing , 2019, 16: 24.
[16] MALY K, SCHIRMER M. The story of CD4+CD28-T cells revisited: solved or still ongoing?[J]. Journal of Immunology Research, 2015, 2015: 348746.
[17] NANDI M, PAL S, GHOSH S, et al. CD8+CD28- T cells: key cytotoxic players impacting disease pathogenesis in chronic HBV infection[J]. Clinical science (London, England : 1979), 2019, 133(17): 1917-1934.
[18] ZHANG Ti, LIU Xin, ZHAO Yue, et al. Excessive IL-15 promotes cytotoxic CD4+CD28- T cell-mediated renal injury in lupus nephritis[J]. Immunity & Ageing, 2022, 19(1): 50.
[19] DEDEOGLU B, MEIJERS R W, KLEPPER M, et al. Loss of CD28 on peripheral T cells decreases the risk for early acute rejection after kidney transplantation[J]. PLoS One, 2016, 11(3): e0150826.
[20] JIA Zhenghu, REN Zhiyao, YE Dongmei, et al. Immune-ageing evaluation of peripheral T and NK lymphocyte subsets in chinese healthy adults[J]. Phenomics (Cham, Switzerland), 2023, 3(4): 360-374.
[21] TANG Guoxing, YUAN Xu, LUO Ying, et al. Establishing immune scoring model based on combination of the number, function, and phenotype of lymphocytes[J]. Aging, 2020, 12(10): 9328-9343.
[22] GLEICHER N, BARAD D H. Gender as risk factor for autoimmune diseases[J]. Journal of Autoimmunity, 2007, 28(1):1-6.
[23] TAKAHASHI T, IWASAKI A. Sex differences in immune responses[J]. Science (New York, N.Y.), 2021, 371(6527): 347-348.

相似文献/References:

[1]姚亚洲,郑引索.72例老年急性白血病免疫表型分析[J].现代检验医学杂志,2015,30(05):91.[doi:10.3969/j.issn.1671-7414.2015.05.027]
　YAO Ya-zhou,ZHENG Yin-suo.Immunophenotype Analysis of 72 Cases of Elderly Acute Leukemia[J].Journal of Modern Laboratory Medicine,2015,30(01):91.[doi:10.3969/j.issn.1671-7414.2015.05.027]
[2]常　玉,刘江鑫,李文生.髓系肉瘤伴T细胞异常表达病例的临床特征、形态学、免疫表型及分子遗传学研究[J].现代检验医学杂志,2022,37(03):11.[doi:10.3969/j.issn.1671-7414.2022.03.003]
　CHANG Yu,LIU Jiang-xin,LI Wen-sheng.Clinical, Morphological, Immunophenotypic and Genetic Analysis of Myeloid Sarcoma with Abnormal T-cell Expression[J].Journal of Modern Laboratory Medicine,2022,37(01):11.[doi:10.3969/j.issn.1671-7414.2022.03.003]
[3]周林颖,刘 兴,伍 勇,等.HIV/AIDS 患者并发EBV 和HCMV 感染临床免疫学特征及影响因素分析[J].现代检验医学杂志,2024,39(02):91.[doi:10.3969/j.issn.1671-7414.2024.02.017]
　ZHOU Linying,LIU Xing,WU Yong,et al.Clinical Immunological Characteristic and Influencing Factors Analysis of HIV/AIDS Patients Infected with EBV and HCMV[J].Journal of Modern Laboratory Medicine,2024,39(01):91.[doi:10.3969/j.issn.1671-7414.2024.02.017]

备注/Memo

备注/Memo:
基金项目：国家自然科学基金项目（82302047）；辽宁省博士科研启动基金（2023-BS-160）；大连医科大学附属第二医院“1+X”计划临床技术水平提升优势技术项目（2022LCJSYS03）。
作者简介：许兆杰（1996-），男，学士，主管技师，研究方向：免疫监控与自身免疫病，E-mail：zhaojie_hsu@163.com。
通迅作者：朱杰（1975-），男，硕士研究生，主任技师，研究方向：血液病诊断与感染性疾病免疫监测，E-mail：13998654975@163.com。
更新日期/Last Update: 2025-01-15