[1]姚亚洲,郑引索.72例老年急性白血病免疫表型分析[J].现代检验医学杂志,2015,30(05):91-93.[doi:10.3969/j.issn.1671-7414.2015.05.027]
　YAO Ya-zhou,ZHENG Yin-suo.Immunophenotype Analysis of 72 Cases of Elderly Acute Leukemia[J].Journal of Modern Laboratory Medicine,2015,30(05):91-93.[doi:10.3969/j.issn.1671-7414.2015.05.027]
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72例老年急性白血病免疫表型分析()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第30卷
期数:
2015年05期
页码:
91-93
栏目:
论著
出版日期:
2015-12-10

文章信息/Info

Title:
Immunophenotype Analysis of 72 Cases of Elderly Acute Leukemia
作者:
姚亚洲; 郑引索
宝鸡市中心医院血液风湿科，陕西宝鸡721008
Author(s):
YAO Ya-zhou; ZHENG Yin-suo
Department of Blood and Rheumatism，Baoji Central Hospital，Shaanxi Baoji 721008，China
关键词:
白血病; 老年; 免疫表型; 流式细胞术
分类号:
R557；R392.11
DOI:
10.3969/j.issn.1671-7414.2015.05.027
文献标志码:
A
摘要:
目的探讨老年急性白血病流式细胞术免疫分型的特点及临床意义。方法回顾性分析2010年1月～2015年3月，利用三色法流式分析法检测76例老年性白血病的免疫表型及其特点。结果①AML中最主要表达的抗原是MPO，CD13和CD33，表达率分别为98.2％，98.2％和7.2％。47.3％有淋系抗原表达，其中CD7(19.3％)，CD56(26.3％)最为常见。②老年B-ALL中，CD19的表达阳性率最高为100％，其次是cCD79a，CD10和CD20，表达阳性率分别为88.9％，46.2％和66.7%。纯B系抗原表达者77.8%，伴T系抗原表达者22.3%，髓系相关抗原CD13，CD33的表达阳性率分别为33.4%和22.3%。③所有老年T-ALL中均表达CD3与CD7，CD5(+)细胞占66.7%，干祖细胞CD34，HLA-DR阳性率分别为33.3％和0％。结论流式细胞术免疫表型分析是一种简便、快速 、准确的检测技术手段，结合传统的检测手段，有助于老年白血病的精确诊断。
Abstract:
ObjectiveTo explore the flow cytometry(FCM) immune classification of elderly acute leukemic characteristics and clinical significance．MethodsAnalysed retrospectively the immunophenotype and the character of 76 patients with elderly acute leukemia，that performed by 3-color flow cytometric analysis method．Results①The major marker in AML were MPO，CD13，CD33，expression rate of 98.2%，98.2% and 77.2%，respectively．②The lymphoid immunophenotypy marker was 47.3% in all patients，including CD7 (19.3%)，CD56 (26.3%．CD19 expression in all older B-lymphoid leukemia，followed by cCD79a，CD10 and CD20 expression positive rate were 88.9%，46.2% and 66.7% respectively．Pure B antigen expression was 77.8%，with T antigen reaches 22.3%．The expression of myeloid associated antigen CD13 was 33.4%，and CD33 positive rate was 22.3%．③The T-cell special marker CD3 and CD7 were in whole T-ALL，CD5 expression positive rate was 66.7%．Only 33.3% cases expressed progenitor cell marker CD34，no had HLA-DR expression．ConclusionFCM immune phenotype analysis is a simple，rapid and accurate means of detection technology，combined with the traditional detection methods，will effected help accurate diagnosis of senile leukemia．

参考文献/References:

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备注/Memo

备注/Memo:
作者简介：姚亚洲(1970-)，男，学士，副主任医师，主要研究方向：血液系统疾病临床研究，E-mail：yaoyazhou001@tom.com。
更新日期/Last Update: 1900-01-01