[1]谢圆媛,杨丹芬.老年COPD患者外周血单核细胞TLR2,TLR4的表达及其与炎症因子的关系研究[J].现代检验医学杂志,2015,30(04):80-83.[doi:10.3969/j.issn.1671-7414.2015.04.022]
　XIE Yuan-yuan,YANG Dan-fen.Study on the Expressions of TLR2,TLR4 in Peripheral Blood Mononuclear Cells and Its Correlation with the Inflammatory Factors Levels of the Elderly Patients with COPD[J].Journal of Modern Laboratory Medicine,2015,30(04):80-83.[doi:10.3969/j.issn.1671-7414.2015.04.022]
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老年COPD患者外周血单核细胞TLR2,TLR4的表达及其与炎症因子的关系研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第30卷
期数:
2015年04期
页码:
80-83
栏目:
论著
出版日期:
2015-08-10

文章信息/Info

Title:
Study on the Expressions of TLR2,TLR4 in Peripheral Blood Mononuclear Cells and Its Correlation with the Inflammatory Factors Levels of the Elderly Patients with COPD
文章编号:
1671-7414(2015)04-080-05
作者:
谢圆媛; 杨丹芬
延安大学附属医院干部病房,陕西延安 716000
Author(s):
XIE Yuan-yuan; YANG Dan-fen
Cadre Ward of the Hospital Affiliated to Yan'an University,Shaanxi Yanan 716000,China
关键词:
老年;  慢性阻塞性肺病;  Toll样受体;  外周血单核细胞;  炎症因子
分类号:
R563; R392.11
DOI:
10.3969/j.issn.1671-7414.2015.04.022
文献标志码:
A
摘要:
目的 观察和分析老年慢性阻塞性肺病(COPD)患者外周血单核细胞TLR2,TLR4等Toll样受体(TLRs)的表达情况及其与炎症因子的关系。方法选取80例老年COPD患者,根据其病情将其分为COPD稳定期组和AECOPD组,分别为43例和37例患者。选取80例健康老年人,根据其是否吸烟分为吸烟组和不吸烟组,分别为41例和39例。对各组研究对象的外周血单核细胞表面TLR2,TLR4表达水平和血清白细胞介素-6(IL-6),白细胞介素-8(IL-8)和肿瘤坏死因子-α(TNF-α)水平进行检测和比较。结果 从AECOPD组、不吸烟组到COPD稳定期组,研究对象的外周血单核细胞表面TLR2,TLR4表达水平依次显著升高,且COPD稳定期组患者的外周血单核细胞表面TLR4表达水平显著高于吸烟组(q=2.815～3.754,P＜0.05); 从AECOPD组,COPD稳定期组、吸烟组到不吸烟组,研究对象的血清IL-6,TNF-α水平依次显著降低且AECOPD组患者的血清IL-8水平显著高于其他三组(q=5.247～8.175,P＜0.05); COPD患者外周血单核细胞表面TLR2的表达水平与血清IL-6水平(标化回归系数=0.458)、血清IL-8水平(标化回归系数=0.622)和血清TNF-α水平(标化回归系数=0.562)具有相关性(P＜0.05),TLR4的表达水平与血清IL-6水平(标化回归系数=0.478)、血清IL-8水平(标化回归系数=0.569)和血清TNF-α水平(标化回归系数=0.352)具有相关性(P＜0.05)。结论 老年COPD患者的外周血单核细胞表面的TLR2,TLR4等TLRs呈现过表达状态,这与患者血清炎症因子水平的上升具有密切的相关性,TLRs的过表达参与了COPD发生和进展的过程。
Abstract:
Objective To observe and analyze the expressions of Toll like receptors(TLRs)of TLR2,TLR4 in peripheral blood mononuclear cells and its correlation with the inflammatory factors levels of the elderly patients with chronic obstructive pulmonary disease(COPD).Methods 80 cases of elderly patients with COPD were selected and divided into the COPD stable phase group and the AECOPD group according to their condition ofdiseases.43 patients and 37 patients were included into each group respectively.80 cases of healthy elderly people were selected and divided into the smokinggroup and the non smoking group.41 cases and 39 cases were included into eachgroup respectively.The expression levels of TLR2,TLR4 in peripheral blood mononuclear cells and the serum levels of interleukin-6(IL-6),interleukin-8(IL-8),tumor necrosis factor-α(TNF-α)of the objects in all the groups weredetected and compared.Results From the AECOPD group,thenon smoking group to the COPD stable phase group,the expression levels of TLR2,TLR4 in peripheral blood mononuclear cells of the objects increased significantly gradually and the TLR4 expression level in peripheral blood mononuclear cells of the patients in the COPD stable phase was significantly higher than that inthe smoking group(q=2.815～3.754,P＜0.05).From the AECOPD group,the COPD stable phase group,the smoking group to the non smoking group,the serum IL-6,TNF-α levels of the objects decreased significantly gradually and the serum IL-8 level of the patients in the AECOPD group was significantly higher thanthat of the other three group(q=5.247～8.175,P＜0.05).The expression level of TLR2 in peripheral blood mononuclear cells of COPD patients was correlated with the serum IL-6 level(standardized regression coefficient=0.458),theserum IL-8 level(standardized regression coefficient=0.622)and the serum TNF-α level(standardized regression coefficient=0.562)(P＜0.05),the expression of TLR4 was correlated with the serum IL-6 level(standard the regression coefficient=0.478),the serum IL-8 level(standardized regression coefficient=0.569)and the serum TNF-α level(standardized regression coefficient=0.352)(P＜0.05).Conclusion The elderly patients with COPD showed the overexpression state of TLRs of TLR2,TLR4 in the peripheral blood mononuclear cells.Such increases were correlated closely with the serum levels of the inflammatory factors in the patients.The overexpression of TLRs shouldinvolve in the occurrence and the progression of COPD.

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备注/Memo

备注/Memo:
作者简介:谢圆媛(1980-),女,本科,主治医师,研究方向:老年呼吸系统疾病的临床诊断,Tel:13409118967。
更新日期/Last Update: 2015-08-10