[1]魏 贤a,何 源a,黄 杰a,等.急性呼吸窘迫综合征新生儿血清miR-138-5p和miR-574-5p水平的变化及临床意义[J].现代检验医学杂志,2025,40(01):69-72+83.[doi:10.3969/j.issn.1671-7414.2025.01.013]
　WEI Xiana,HE Yuana,HUANG Jiea,et al.Changes and Clinical Significance of Serum miR-138-5p and miR-574-5p Levels in Newborns Acute Respiratory Distress Syndrome[J].Journal of Modern Laboratory Medicine,2025,40(01):69-72+83.[doi:10.3969/j.issn.1671-7414.2025.01.013]
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急性呼吸窘迫综合征新生儿血清miR-138-5p和miR-574-5p水平的变化及临床意义()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第40卷
期数:
2025年01期
页码:
69-72+83
栏目:
论著
出版日期:
2025-01-15

文章信息/Info

Title:
Changes and Clinical Significance of Serum miR-138-5p and miR-574-5p Levels in Newborns Acute Respiratory Distress Syndrome
文章编号:
1671-7414（2025）01-069-05
作者:
魏 贤a; 何 源a; 黄 杰a; 冯 卓a; 王 锋b
（武汉科技大学附属孝感医院a. 新生儿科；b. 检验科，湖北孝感432100）
Author(s):
WEI Xiana; HE Yuana; HUANG Jiea; FENG Zhuoa; WANG Fengb
（a . Department of Neonatology；b. Department of Clinical Laboratory， Xiaogan Hospital Affiliated to Wuhan University of Science and Technology, Hubei Xiaogan 432100，China）
关键词:
新生儿急性呼吸窘迫综合症; 微小RNA-138-5p; 微小RNA-574-5p
分类号:
R722.1；R392.11
DOI:
10.3969/j.issn.1671-7414.2025.01.013
文献标志码:
A
摘要:
目的 探讨新生儿急性呼吸窘迫综合征（nARDS）患儿血清miR-138-5p 和miR-574-5p 表达水平的变化及临床意义。方法 收集2018 年9 月～ 2023 年8 月武汉科技大学附属孝感医院新生儿科收治的nARDS 患儿112 例作为观察组，并根据预后分为预后良好组（n=98）和预后不良组（n=14）；选择同期出生的足月健康新生儿104 例作为对照组。定量实时聚合酶链反应（qRT-PCR）法检测血清miR-138-5p 和miR-574-5p 的表达水平；ROC 曲线分析血清miR-574-5p，miR-138-5p 水平对nARDS 患儿预后的诊断价值；多因素Logistic 回归分析影响nARDS 患儿预后不良的因素。结果 与对照组比较，观察组血清miR-574-5p 表达水平（1.72±0.23 vs 1.04±0.17）升高，miR-138-5p 表达水平（0.55±0.08vs 1.02±0.16）降低，差异具有统计学意义（t=24.557，25.597，均P ＜ 0.05）；与预后良好组比较，预后不良组血清miR-138-5p 表达水平（0.41±0.06 vs 0.57±0.08）降低，miR-574-5p 表达水平（2.07±0.25 vs 1.67±0.19）升高，差异具有统计学意义（t=7.188，7.069，均P ＜ 0.05）；预后不良组产妇年龄、宫内窘迫、羊水异常比例较预后良好组升高，差异具有统计学意义（t=5.359，4.257，6.577，均P ＜ 0.05）。miR-574-5p 是nARDS 患儿预后不良的独立危险因素，miR-138-5p 是保护因素（均P ＜ 0.05）。血清miR-138-5p，miR-574-5p 单独及联合诊断nADRS 患儿预后不良的AUC（95%CI）分别为0.793(0.706 ～ 0.864)，0.898(0.826 ～ 0.947) 和0.931(0.867 ～ 0.970)，二者联合预测优于miR-138-5p，miR-574-5p 各自单独诊断（Z=2.151，1.982，均P ＜ 0.05）。结论 nARDS 患儿血清miR-138-5p 表达水平降低，miR-574-5p 表达水平升高，二者对nARDS 患儿预后不良具有一定的诊断价值。
Abstract:
Objective To investigate the changes of serum microRNA (miR) -138-5p and microRNA（miR）-574-5p expression levels and their clinical significance in newborns acute respiratory distress syndrome (nARDS). Methods A total of 112 infants with nARDS from September 2018 to August 2023 in the Neonatal Department of Xiaogan Hospital Affiliated to Wuhan University of Science and Technology were collected as the observation group. They were divided into good prognosis group (n=98) and the poor prognosis group (n=14). A total of 104 healthy full-term newborns born at the same time were selected as the control group. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to detect the expression levels of miR-138-5p and miR-574-5p in serum. ROC curve was used to analyze the diagnostic value of serum miR-574-5p and miR- 138-5p levels for poor prognosis of infants with nARDS. Multivariate Logistic regression analysis of factors affecting the poor prognosis of infants with nARDS. Results Compared with the control group, the expression level of serum miR-574-5p in the observation group increased (1.72 ± 0.23 vs 1.04 ± 0.17), with the expression level of miR-138-5p decreased (0.55 ± 0.08 vs 1.02 ± 0.16), with statistically significant differences (t=24.557, 25.597, all P<0.05). Compared with the good prognosis group, the serum miR-138-5p expression level in the poor prognosis group decreased (0.41 ± 0.06 vs 0.57 ± 0.08), with the miR-574- 5p expression level increased (2.07 ± 0.25 vs 1.67 ± 0.19), with statistically significant differences (t=7.188, 7.069, all P<0.05). The age，intrauterine distress，and proportion of abnormal amniotic fluid in the poor prognosis group were higher than those in the good prognosis group (t=5.359, 4.257, 6.577, all P<0.05). MiR-574-5p was an independent risk factor for poor prognosis with nARDS , and miR-138-5p was a protective factor (all P<0.05).The AUC (95% CI) of serum miR-138-5p and miR-574-5p alone and combined diagnosis of nADRS were 0.793 (0.706 ～ 0.864), 0.898 (0.826 ～ 0.947) and 0.931 (0.867 ～ 0.970), respectively. The combined prediction of miR-138-5p and miR-574-5p were better than that of miR-138-5p alone and miR-574- 5p alone (Z=2.151，1.982， all P<0.05). Conclusion The expression levels of miR-138-5p in serum of infant with nARDS were lower and the expression levels of miR-574-5p were higher, both of which have certain diagnostic value for poor prognosis of infant with nARDS .

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备注/Memo

备注/Memo:
基金项目：孝感市科学技术局资助项目（编号：XGKJ2021010080）。
作者简介：魏贤（1983-），女，硕士研究生，副主任医师，研究方向：新生儿呼吸系统疾病，早产儿营养及神经发育评估，E-mail：r43ykb@163.com。
更新日期/Last Update: 2025-01-15