[1]安立娟,张 翔,庞学成,等.子宫内膜癌组织中LGALS3BP mRNA，G3BP1 mRNA的表达与Wnt/β-catenin通路基因及临床病理学特征的相关性研究[J].现代检验医学杂志,2025,40(04):43-49.[doi:10.3969/j.issn.1671-7414.2025.04.008]
　AN Lijuan,ZHANG Xiang,PANG Xuecheng,et al.Expression of LGALS3BP mRNA, G3BP1 mRNA in Endometrial Cancer Tissue and Their Correlation with the Wnt/β-catenin Pathway Genes and Clinical Pathological Characteristics[J].Journal of Modern Laboratory Medicine,2025,40(04):43-49.[doi:10.3969/j.issn.1671-7414.2025.04.008]
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子宫内膜癌组织中LGALS3BP mRNA，G3BP1 mRNA的表达与Wnt/β-catenin通路基因及临床病理学特征的相关性研究()

《现代检验医学杂志》[ISSN:/CN:]

卷:
第40卷
期数:
2025年04期
页码:
43-49
栏目:
论著
出版日期:
2025-07-15

文章信息/Info

Title:
Expression of LGALS3BP mRNA, G3BP1 mRNA in Endometrial Cancer Tissue and Their Correlation with the Wnt/β-catenin Pathway Genes and Clinical Pathological Characteristics
文章编号:
1671-7414（2025）04-043-07
作者:
安立娟，张 翔，庞学成，钱素敏，杨伟伟
（沧州市中心医院妇科，河北沧州 061000）
Author(s):
AN Lijuan, ZHANG Xiang, PANG Xuecheng, QIAN Sumin, YANG Weiwei
(Department of Gynaecology, Cangzhou Central Hospital, Hebei Cangzhou 061000, China)
关键词:
子宫内膜癌; 半乳糖凝集素-3 结合蛋白; GTP 酶激活蛋白SH3 功能区结合蛋白1; Wnt/β-catenin通路
分类号:
R737.33；R730.43
DOI:
10.3969/j.issn.1671-7414.2025.04.008
文献标志码:
A
摘要:
目的 探讨子宫内膜癌（EC）组织中半乳糖凝集素-3 结合蛋白（LGALS3BP）、GTP 酶激活蛋白SH3 功能区结合蛋白1（G3BP1）表达与Wnt/β-catenin 通路基因的相关性及临床预后意义。方法 选择2016 年2 月~2019 年2月沧州市中心医院诊治的138 例EC 患者。采用实时荧光定量PCR(qRT-PCR) 检测癌和癌旁组织LGALS3BP mRNA，G3BP1 mRNA 及Wnt/β-catenin 通路基因Wnt5a mRNA，β-catenin mRNA，基质金属蛋白酶-9（MMP-9）mRNA 表达。采用免疫组织化学法(IHC) 检测癌和癌旁组织LGALS3BP 蛋白、G3BP1 蛋白表达。Pearson 相关分析LGALS3BPmRNA，G3BP1 mRNA 及Wnt/β-catenin 通路基因的相关性。利用Kaplan-Meier 曲线分析LGALS3BP mRNA，G3BP1mRNA 对EC 患者生存预后的影响。COX 回归模型分析影响EC 患者预后的因素。结果 EC 癌组织中 LGALS3BPmRNA（3.01±0.34），G3BP1 mRNA（2.87±0.33），Wnt5a mRNA(2.29±0.26)，β-catenin mRNA(3.25±0.41)，MMP-9 mRNA(2.68±0.36) 表达均高于癌旁组织(1.10±0.23，1.06±0.24，0.84±0.17，0.88±0.26，0.69±0.17），差异具有统计学意义（t=52.109 ～ 58.719，均P ＜ 0.001）。EC 中LGALS3BP mRNA，G3BP1 mRNA 与Wnt5a mRNA，β-cateninmRNA，MMP-9 mRNA 表达呈正相关（r=0.675 ～ 0.781，均P ＜ 0.001）。EC 癌组织中LGALS3BP 蛋白（3.54±0.47），G3BP1 蛋白（2.84±0.44）表达高于癌旁组织（0.51±0.16，0.42±0.13），差异具有统计学意义（t=71.692，61.962，均P ＜ 0.001）。FIGO 分期Ⅲ期、淋巴结转移EC 患者癌组织LGALS3BP mRNA，G3BP1 mRNA 表达高于FIGO 分期Ⅰ ~ Ⅱ期、无淋巴结转移患者，差异具有统计学意义（t=40.279 ～ 557.671，均P <0.001）。LGALS3BP mRNA 高表达组5 年生存率为58.82%（40/68），低于低表达组的94.29%（66/70），差异具有统计学意义（Log-rank χ2=24.970，P＜ 0.001）；G3BP1 mRNA 高表达组5 年生存率为62.12%（41/66），低于低表达组的90.28%（65/72），差异具有统计学意义（Log-rankχ2=15.960，P ＜ 0.001）。FIGO 分期Ⅲ期、淋巴结转移、LGALS3BP mRNA 高表达、G3BP1 mRNA 高表达是EC 患者不良预后的危险因素（Wald χ2=7.847~12.054，均P<0.001）。结论 EC 中LGALS3BP，G3BP1 mRNA 表达升高，两者均与Wnt/β-catenin 通路基因表达呈正相关，促进EC 肿瘤的恶性进展，是新的评估EC 患者预后的肿瘤标志物。
Abstract:
Objective To investigate the correlation between the expression of lectin galactoside binding soluble-3 binding protein (LGALS3BP), GTP enzyme activating protein SH3 functional region binding protein 1(G3BP1） and Wnt / β-catenin pathway genes in endometrial cancer (EC） tissues and its clinical prognostic significance. Methods 138 patients with EC treated in Cangzhou Central Hospital from February 2016 to February 2019 were selected. qRT-PCR was used to detect the expression of LGALS3BP mRNA, G3BP1 mRNA and Wnt / β-catenin pathway genes Wnt5a mRNA, β-catenin mRNA and matrix metalloproteinase-9 (MMP-9） mRNA in cancer and adjacent tissues.The expression of LGALS3BP protein and G3BP1 protein in cancer and adjacent tissues was detected by immunohistochemistry.Pearson correlation analysis was used to analyze the correlation between LGALS3BP mRNA, G3BP1 mRNA and Wnt / β-catenin pathway genes.Kaplan-Meier curve was used to analyze the effect of LGALS3BP mRNA and G3BP1 mRNA on the survival and prognosis of EC patients. COX regression model was used to analyze the factors affecting the prognosis of EC patients. Results The expression of LGALS3BP mRNA(3.01± 0.34), G3BP1 mRNA(2.87±0.33), Wnt5a mRNA(2.29±0.26), β-catenin mRNA(3.25±0.41) and MMP-9 mRNA(2.68±0.36) in EC cancer tissues were higher than those in adjacent tissues(1.10±0.23，1.06±0.24，0.84±0.17，0.88±0.26，0.69±0.17), and the differences were statistically significant (t = 52.109 ~ 58.719, all P < 0.001）.The expression of LGALS3BP mRNA and G3BP1 mRNA in EC was positively correlated with the expression of Wnt5a mRNA, β-catenin mRNA and MMP-9 mRNA (r = 0.675~0.781, all P < 0.001）.The expression of LGALS3BP protein (3.54 ± 0.47 vs 0.51 ± 0.16） and G3BP1 protein (2.84 ± 0.44 vs 0.42 ± 0.13） in EC cancer tissues were higher than that in adjacent tissues, and the differences were statistically significant (t = 71.692,61.962, all P < 0.001）.The expression of LGALS3BP mRNA and G3BP1 mRNA in cancer tissues of EC patients with FIGO stage III and lymph node metastasis were higher than that of FIGO stage I ~ II and no lymph node metastasis, and the differences were statistically significant (t = 40.279 ~ 557.671, all P < 0.001）.The 5-year survival rate of LGALS3BP mRNA high expression group was 58.82 % (40 / 68）, which was lower than that of low expression group 94.29 % (66 / 70）, and the difference was statistically significant (Log-rank χ2 = 24.970, P < 0.001）.The 5-year survival rate of G3BP1 mRNA high expression group was 62.12 % (41 / 66）, which was lower than that of low expression group 90.28 % (65 / 72）, and the difference was statistically significant (Log-rank χ2= 15.960, P < 0.001）.FIGO stage III, lymph node metastasis, high expression of LGALS3BP mRNA and high expression of G3BP1 mRNA were risk factors for poor prognosis of EC patients (Wald χ2=7.847~12.054，all P < 0.001）. Conclusion The expression of LGALS3BP and G3BP1 mRNA is elevated in EC, both of which are associated with Wnt/ β- catenin pathway genes, promoting the malignant progression of EC tumors, and are new tumor markers for evaluating the prognosis of EC patients.

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备注/Memo

备注/Memo:
基金项目：河北省“三三三人才工程”资助项目（C20221122）。
作者简介：安立娟（1982-），女，硕士研究生，主治医师，研究方向：妇科肿瘤，E-mail：anlijuan820226@163.com。
通讯作者：杨伟伟，男，硕士研究生，主治医师，研究方向：妇科肿瘤，E-mail:1145293016@qq.com。
更新日期/Last Update: 2025-07-15