[1]赵玉梅,张月月,秦晓琨,等.不同染色过程对提升流式细胞仪检测外周血自然杀伤细胞的效果评价[J].现代检验医学杂志,2026,41(01):165-169.[doi:10.3969/j.issn.1671-7414.2026.01.032]
　ZHAO Yumei,ZHANG Yueyue,QIN Xiaokun,et al.Evaluation of the Effectiveness of Different Staining Processes on Enhancing Flow Cytometry Detection of Peripheral Blood Natural Killer Cells[J].Journal of Modern Laboratory Medicine,2026,41(01):165-169.[doi:10.3969/j.issn.1671-7414.2026.01.032]
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不同染色过程对提升流式细胞仪检测外周血自然杀伤细胞的效果评价()

《现代检验医学杂志》[ISSN:/CN:]

卷:
第41卷
期数:
2026年01期
页码:
165-169
栏目:
论著
出版日期:
2026-01-15

文章信息/Info

Title:
Evaluation of the Effectiveness of Different Staining Processes on Enhancing Flow Cytometry Detection of Peripheral Blood Natural Killer Cells
文章编号:
1671-7414（2026）01-165-05
作者:
赵玉梅1，张月月2，秦晓琨3，薛国庆3，高华方1
1.国家卫生健康委科学技术研究所国家人类遗传资源中心，北京100081;2.北京大学国际医院检验科，北京102218;3.北京指真生物科技有限公司研发部，北京100176
Author(s):
ZHAO Yumei1，ZHANG Yueyue2，QIN Xiaokun3，XUE Guoqing3，GAO Huafang1
1.National Center for Human Genetic Resources, National Research Institute for Health and Family Planning, Beijing 100081, China;2.Department of Clinical Laboratory, Peking University International Hospital, Beijing 102218, China;3.Department of R&D, Beijing Zhizhen Biological Science and Technology Co. Ltd, Beijing 100176, China
关键词:
流式细胞术; 染色; 自然杀伤细胞; 细胞分群
分类号:
R446
DOI:
10.3969/j.issn.1671-7414.2026.01.032
文献标志码:
A
摘要:
目的分析两种染色过程对人外周血中的自然杀伤（NK）细胞检测效果的影响。方法选取2024年6月在北京大学国际医院体检中心诊疗的66例不同免疫水平患者作为研究对象，其中男性33例，女性33例，分别使用两个厂商的试剂，每种试剂共涉及两种染色过程（直接染色和先洗涤后染色），通过流式细胞术检测淋巴细胞免疫表型CD3+百分比和CD3-(CD56+CD16)+百分比，比较两种染色过程淋巴细胞免疫表型的差异性；同时分析NK细胞分离度来考察两种染色过程对NK细胞分离效果的影响。结果两种试剂结果均显示CD3+百分比和CD3-(CD56+CD16)+百分比在两种染色过程差异无统计学意义（Z=-1.793～-0.379,均P>0.05）。国产试剂在两种染色过程检测CD3+百分比的组内相关系数（ICC）为0.977，CD3-(CD56+CD16)+百分比的ICC为0.990；进口试剂在两种染色过程检测CD3+百分比的ICC为0.977，CD3-(C-D56+CD16)+百分比的ICC为0.991，两种试剂均具有较好一致性。两种试剂结果均显示NK细胞分离度在两种染色过程差异具有统计学意义（Z=-6.624、-6.736，均P<0.0001），与直接染色相比，先洗涤后染色NK细胞分离度更大。结论两种染色过程测试得到的CD3+百分比和CD3-(CD56+CD16)+百分比一致性较好，针对NK细胞分群较差或无法分群的标本可以尝试先洗涤后染色，改善CD3-(CD56+CD16)+细胞群与CD3-(CD56+CD16)-细胞群的分离效果，从而提升NK细胞检测准确度，为临床提供辅助诊断价值。
Abstract:
Objective To analyze the effects of two staining processes on the detection of natural killer (NK) cells in human pe-ripheral blood. Methods A total of 66 patients with different immune levels who were diagnosed and treated at the Physical Ex-amination Center of Peking University International Hospital in June 2024 were enrolled as the research subjects, including 33 males and 33 females. Reagents from two manufacturers were used, each involving two staining processes (direct staining and washing followed by staining). The lymphocyte immunophenotype percentage of CD3+and CD3-(CD56+CD16)+ were detected by flow cytometry, and the differences in lymphocyte immunophenotypes between the two staining processes were compared. Simultaneously, NK cells isolation efficienty was analyzed to examine the impact of the two staining processes on NK cells separation. Results Both reagents showed no statistically significant differences in CD3+ percentage and CD3-(CD56+CD16)+percentage between the two staining processes (Z= -1.793～-0.379, all P>0.05). The intraclass correlation coefficients (ICC) for CD3+ percentage and CD3-(CD56+CD16)+ percentage detected by the domestic reagents across two staining processes were 0.977 and 0.990, respectively. The ICCs for detecting percentages of CD3+and CD3-(CD56+CD16)+ using the imported reagents in both staining processes were 0.977 and 0.991, respectively, indicating good consistency for both reagents. The results from both reagents showed statistically significant differences in NK cell separation rates between the two staining processes (Z= -6.624,-6.736, all P<0.000 1). Compared with the direct staining, NK cell separation rates were higher after washing followed bystain-ing. Conclusions The CD3+ percentage and CD3-(CD56+CD16)+ percentage obtained from both staining processes showed good consistency. For specimens with poor NK cell separation or those that can’t be separated, attempting washing followed by-stain-ing may improve the separation of CD3-(CD56+CD16)+ and CD3-(CD56+CD16)- cell populations. This approach enhances accuracy of NK cell detection and provides auxiliary diagnostic value for clinical practice.

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备注/Memo

备注/Memo:
作者简介：赵玉梅（1983-），女，硕士，高级工程师，研究方向：检验医学、免疫学，E-mail：aux1983@163.com。
更新日期/Last Update: 2026-01-15