[1]刘军霞,唐伟锋,刘晓宁,等.ISIS与ICP-MS耦联检测儿童全血11种微量元素的方法建立及应用研究[J].现代检验医学杂志,2026,41(02):16-22.[doi:10.3969/j.issn.1671-7414.2026.02.004]
　LIU Junxia,TANG Weifeng,LIU Xiaoning,et al.Establishment and Application of a Method for Detecting 11 Trace Elements in Children’s Whole Blood Using ICP-MS Coupled with ISIS[J].Journal of Modern Laboratory Medicine,2026,41(02):16-22.[doi:10.3969/j.issn.1671-7414.2026.02.004]
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ISIS与ICP-MS耦联检测儿童全血11种微量元素的方法建立及应用研究()

《现代检验医学杂志》[ISSN:/CN:]

卷:
第41卷
期数:
2026年02期
页码:
16-22
栏目:
论著
出版日期:
2026-03-15

文章信息/Info

Title:
Establishment and Application of a Method for Detecting 11 Trace Elements in Children’s Whole Blood Using ICP-MS Coupled with ISIS
文章编号:
1671-7414（2026）02-016-07
作者:
刘军霞a，唐伟锋a，刘晓宁a，黄 贤a，袁理利a，敖俊杰a，沈袁恒b，张前龙a
上海交通大学医学院附属新华医院 a.环境与儿童健康教育部与上海市重点实验室；b.检验科，上海 200092
Author(s):
LIU Junxiaa, TANG Weifenga, LIU Xiaoninga, HUANG Xiana, YUAN Lilia, AO Junjiea, SHEN Yuanhengb, ZHANG Qianlonga
a. Ministry of Education-Shanghai Key Laboratory of Children’s Environmental Health; b. Department of Clinical Laboratory, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine , Shanghai 200092, China
关键词:
微量元素; 电感耦合等离子体质谱法; 一体化进样系统; 儿童
分类号:
R446.11；Q503
DOI:
10.3969/j.issn.1671-7414.2026.02.004
文献标志码:
A
摘要:
目的建立一体化进样系统(ISIS)与电感耦合等离子体质谱法(ICP-MS)联用的快速检测全血11种微量元素的方法,并在儿童群体中进行初步应用。方法采用直接稀释法前处理样本,ISIS耦联ICP-MS检测200例儿童全血样本,并对方法的线性范围、检出限、定量限、精密度及准确度进行评估。结果11种微量元素的线性范围为0.10~500.00ng/ml,相关系数(r?)均>0.990;检出限范围为0.02~0.89ng/ml,定量限范围为0.06~2.95ng/ml;低、中、高浓度加标样本的日内和日间精密度均<15.00%,回收率为89.70%~114.96%。200例儿童血样有毒重金属铅、汞、砷的超标率分别为1.00%、4.50%和7.00%。结论ISIS耦联ICP-MS检测全血中11种微量元素性能良好,在儿童微量元素评估中具有较高的应用价值。
Abstract:
Objective To develop a rapid method for detecting 11 trace elements in whole blood using inductively coupled plasma mass spectrometry (ICP-MS) coupled with an integrated injection system (ISIS), and to preliminarily assess its application in pedi-atric populations. Methods Whole blood samples were pretreated using a direct dilution approach, and 200 pediatric samples were analyzed using ICP-MS coupled with ISIS. The method's performance was evaluated based on linearity, limit of detection (LOD), limit of quantification (LOQ), precision and accuracy. Results The linear range of 11 trace elements was 0.10~500.00 ng/ml, with correlation coefficients (r?) exceeding 0.990 for all parameters. The LOD of 11 trace elements ranged from 0.02 to 0.89 ng/ml, and the LOQ ranged from 0.06 to 2.95 ng/ml. The relative standard deviations (RSD) of intra-day and inter-day for 11 trace elements with low, middle, high recovery concentrations were all less than 15.00%, with recovery rates ranging from 89.70% to 114.96%. Among 200 pediatric blood samples, the exceedance rates for toxic heavy metals lead, mercury and arsenic were 1.00%, 4.50% and 7.00%, respectively. Conclusions The coupling of ISIS with ICP-MS demonstrates excellent performance in detecting 11 trace ele-ments in whole blood, and holds high application value for the assessment of trace elements in children.

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相似文献/References:

[1]招湛静,刘爱胜,刘爱玲,等.深圳地区0～7岁儿童微量元素含量现状调查[J].现代检验医学杂志,2017,32(03):149.[doi:10.3969/j.issn.1671-7414.2017.03.042]
　ZHAO Zhan-jing,LIU Ai-sheng,LIU Ai-ling,et al.Present Situation Investigation of Trace Elements of 0~7 Years Old Children in Shenzhen District[J].Journal of Modern Laboratory Medicine,2017,32(02):149.[doi:10.3969/j.issn.1671-7414.2017.03.042]

备注/Memo

备注/Memo:
基金项目：上海市加强公共卫生体系建设三年行动计划重点学科建设项目（GWVI-11.1-41）。
作者简介：刘军霞（1986-），女，医学硕士，主管技师，研究方向：无机质谱检测，E-mail：ljxntmc3312@qq.com。
通讯作者：张前龙（1979-），男，医学硕士，副主任技师，研究方向：环境污染物与人群健康研究，E-mail：zhangql7989@163.com。
更新日期/Last Update: 2026-03-15