[1]张 宁,何晓璇,孟 欣.血细胞分析仪WDF和WNB散点图在血小板数量检测质量管理中的应用[J].现代检验医学杂志,2026,41(02):187-191.[doi:10.3969/j.issn.1671-7414.2026.02.031]
　ZHANG Ning,HE Xiaoxuan,MENG Xin.Application of WDF and WNB Scatter Plots in Quality Management of Platelet Count Detection Using Hematology Analyzers[J].Journal of Modern Laboratory Medicine,2026,41(02):187-191.[doi:10.3969/j.issn.1671-7414.2026.02.031]
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血细胞分析仪WDF和WNB散点图在血小板数量检测质量管理中的应用()

《现代检验医学杂志》[ISSN:/CN:]

卷:
第41卷
期数:
2026年02期
页码:
187-191
栏目:
质量控制·实验室管理
出版日期:
2026-03-15

文章信息/Info

Title:
Application of WDF and WNB Scatter Plots in Quality Management of Platelet Count Detection Using Hematology Analyzers
文章编号:
1671-7414（2026）02-187-05
作者:
张 宁，何晓璇，孟 欣
西安交通大学第一附属医院检验科，西安710061
Author(s):
ZHANG Ning，HE Xiaoxuan，MENG Xin
Department of Clinical Laboratory, the First Affiliated Hospital of Xi’an Jiaotong University, Xi’an 710061, China
关键词:
散点图; 血细胞分析仪; 血小板; 复检流程
分类号:
R-331；R446
DOI:
10.3969/j.issn.1671-7414.2026.02.031
文献标志码:
A
摘要:
目的探讨并验证白细胞WDF通道散点图(WDF)和白细胞WNB通道散点图(WNB)在监测血液微小凝集、大血小板及血小板聚集中的价值。方法收集2023年4~12月西安交通大学第一附属医院EDTA-K2血液样本1788例,Min-drayBC-6800Plus全自动血细胞分析仪触发血小板聚集报警,或血小板直方图异常,或WDF、WNB散点图Ghost区域异常时,使用MC-80全自动血细胞形态学分析仪判断不同异常报警与血液微小凝集、血小板聚集及大血小板的关系。结果173例WDF散点图Ghost区域异常样本,血小板数量减低,与重新采集血液前比较,重新采集血液后血小板数量明显增高,差异具有统计学意义(Z=-10.009,P<0.05)。1252例WNB散点图Ghost区域异常的样本,MC-80全自动血细胞形态学分析系统镜检提示大血小板1035例(占比82.67%)、血小板聚集217例(占比17.33%),平均血小板体积(MPV)、大血小板比率(P-LCR)、血小板分布宽度标准差(PDW-SD)血小板聚集样本明显低于大血小板样本,差异具有统计学意义(t=9.785、8.682、8.344,均P<0.05)。血小板压积(PCT)、大血小板数量(P-LCC)血小板聚集样本高于大血小板样本,差异无统计学意义(t=-1.490、-0.777,均P>0.05)。200例大血小板样本,电阻抗法血小板(PLT-I)计数为100(84,127)×109/L,光学血小板(PLT-O)计数为104(85,132)×109/L,PLT-O高于PLT-I计数,差异无统计学意义(Z=-0.280,P>0.05)。217例WNB散点图Ghost区域异常的血小板聚集样本,PLT-I计数为95(77,119)×109/L,PLT-O计数为110(89,146)×109/L,PLT-O高于PLT-I计数,差异具有统计学意义(Z=-2.987,P<0.05)。结论血细胞分析检测及报告审核过程应结合血细胞计数、WDF散点图、WNB散点图、血小板直方图等信息,合理制定血小板复检规则及流程,必要时结合形态学分析结果综合判断,以保证血细胞分析检验结果准确可靠。
Abstract:
Objective To explore and validate the value of WBC WDF Scattergram (WDF) and WBC NRBC BASO Scattergram (WNB) in monitoring blood microagglutination, large platelets and platelet aggregation. Methods A total of 1 788 EDTA-K2 blood samples were collected from the First Affiliated Hospital of Xi’an Jiaotong University between April 2023 and December 2023. When the Mindray BC-6800 Plus automatic hematology analyzers triggered the platelet agglutination alarms, exhibited ab-normal platelet histograms, or showed abnormal Ghost regions in WDF / WNB scatter plots, the MC-80 automated morphology analyzer was used to assess the relationship between different abnormal alarms and microagglutination, platelet aggregation and large platelet. Results Among 173 samples with abnormal Ghost regions in WDF scatter plots, platelet counts decreased. Com-pared to pre-recollection levels, platelet counts significantly increased after recollection (Z=-10.009, P<0.05). Among 1252 sam-ples with abnormal Ghost zone in WNB scatter plots, the MC-80 automatic morphology analyzer revealed large platelet in 1035 cases (82.67%), and platelet aggregation in 217 cases (17.33%). There was statistically significant difference in the mean platelet volume (MPV), large platelet count ratio (P-LCR), and platelet distribution width standard deviation (PDW-SD) were significant-ly lower in platelet aggregation samples than in large platelet samples (t=9.785, 8.682, 8.344, all P<0.05). Platelet count (PCT) and large platelet count (P-LCC) were higher in platelet aggregation samples than in large platelets samples, with no statistically significant differences (t=-0.777, -1.490, all P>0.05). Among 200 samples with large platelet, the impedance-based platelet count (PLT-I) was 100 (84, 127)×109/L, while the optical platelet count (PLT-O) was 104 (85, 132)×109/L. There was no statistically significant difference between PLT-I and PLT-O (Z=-0.280, P>0.05). Among 217 platelet aggregation samples with abnormal Ghost regions on WNB scatter plots, the PLT-I was 95 (77, 119)×109/L and the PLT-O was 110 (89, 146)×109/L. PLT-O counts were significantly higher than PLT-I counts (Z=-2.987, P<0.05). Conclusions The process of hematology analysis and report review should integrate information from blood cell counts, WDF and WNB scatter plots, and platelet histograms to establish reasonable rules and procedures for platelet reexamination. It will be necessary to incorporate morphological analysis results for comprehensive judgment to ensure the accuracy and reliability of hematology analysis results.

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备注/Memo

备注/Memo:
基金项目：陕西省重点研发计划项目（2021SF-343）。
作者简介：张宁（1981-），男，硕士研究生，主任检验师，研究方向：临床血液体液学检验，E-mail：237474800@qq.com。
通讯作者：孟欣（1980-），女，硕士研究生，副主任检验师，研究方向：临床血液学检验，E-mail：468237221@qq.com。
更新日期/Last Update: 2026-03-15