[1]王 珊,吕 媛.分子生物学方法在肠球菌耐药性研究中的应用[J].现代检验医学杂志,2015,30(01):4-7.[doi:10.3969/j.issn.1671-7414.2015.01.002]
　WANG Shan,LU Yuan.Application of Molecular Biology in Investigation of Resistance in Enterococci[J].Journal of Modern Laboratory Medicine,2015,30(01):4-7.[doi:10.3969/j.issn.1671-7414.2015.01.002]
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分子生物学方法在肠球菌耐药性研究中的应用()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第30卷
期数:
2015年01期
页码:
4-7
栏目:
述评
出版日期:
2015-01-30

文章信息/Info

Title:
Application of Molecular Biology in Investigation of Resistance in Enterococci
文章编号:
1671-7414(2015)01-004-04
作者:
王 珊; 吕 媛
北京大学第一医院临床药理研究所,北京 100191
Author(s):
WANG Shan; LU Yuan
Institute of Clinical Pharmacology,the First Hospital of Peking University,Beijing100191,China
关键词:
肠球菌;  耐药性;  分子生物学;  万古霉素耐药肠球菌
分类号:
R378.1; R392.11
DOI:
10.3969/j.issn.1671-7414.2015.01.002
文献标志码:
A
摘要:
目的 为了及时准确地检测细菌的耐药性,该文将肠球菌耐药性研究中的分子生物学方法进行综述。方法 对近年来肠球菌耐药性研究的相关文献进行整理、分析与归纳。结果 一些方法比较常见,如PCR,PFGE,MSLT和Southern杂交等,而一些方法如焦磷酸测序和基因芯片技术,目前在细菌耐药机制研究中的应用并不广泛,属于交叉学科,但是稍加利用会有一定的发展前景。结论 任何一个方法都有优点,也存在一些局限性,应取长补短,发挥每种方法的最大优势。
Abstract:
Objective This article aims to provide mechanisms and recent developments of molecular biology pertaining to resistance of Enterococci,providing rapid approaches for detecting resistant strains.Methods This article reviewed recent literatures on resistanceof Enterococci and a systemic analysis was conducted.ResultsCommon detecting methods include polymerase chain reaction(PCR),pulsed field gel electrophoresis(PFGE),multilocus sequence typing(MLST)and Southern blot.There also exist less widely-used methods such as pyrosequencing andgenechip technique,which may prove efficient in some aspects.Conclusion Every method has its advantages and disadvantages.This article discussed how to utilize these methods to achieve their maximum capabilities.

参考文献/References:

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备注/Memo

备注/Memo:

作者简介:王 珊(1981-),女,硕士,助理研究员,从事细菌耐药机制研究,Tel:13691189003,E-mail:tigerwangshan@126.com。
通讯作者:吕 媛,女,研究员,博士生导师,从事临床药理学研究,E-mail:lyzx5857@163.com。
更新日期/Last Update: 2015-01-30