[1]林 江a,王 珂a,陈孙云b.自身免疫性肝病和病毒性肝炎患者血清中抗肝抗体检测的临床意义[J].现代检验医学杂志,2015,30(03):153-156.[doi:10.3969/j.issn.1671-7414.2015.03.047]
　LIN Jianga,WANG Kea,CHEN Sun-yunb.Clinical Significance of Detection Antibody of Liver Antigens in Patients with Autoimmune Hepatic Diseases and Viral Hepatitis[J].Journal of Modern Laboratory Medicine,2015,30(03):153-156.[doi:10.3969/j.issn.1671-7414.2015.03.047]
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自身免疫性肝病和病毒性肝炎患者血清中抗肝抗体检测的临床意义()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第30卷
期数:
2015年03期
页码:
153-156
栏目:
检验与临床
出版日期:
2015-06-10

文章信息/Info

Title:
Clinical Significance of Detection Antibody of Liver Antigens in Patients with Autoimmune Hepatic Diseases and Viral Hepatitis
文章编号:
1671-7414(2015)03-153-04
作者:
林 江a; 王 珂a; 陈孙云b
东南大学医学院附属江阴医院 a.检验科; b.感染科,江苏江阴 214400
Author(s):
LIN Jianga; WANG Kea; CHEN Sun-yunb
a.Department of Clinical Laboratory;
b.Department of Infectious Diseases,Jiangyin People's Hospital Affiliated to Medical School of Southeast University,Jiangsu Jiangyin 214400,China
关键词:
自身免疫性肝病;  免疫印迹法;  自身抗体
分类号:
R512.6; R575; R392.11
DOI:
10.3969/j.issn.1671-7414.2015.03.047
文献标志码:
A
摘要:
目的 探讨抗肝原几种抗体在自身免疫性肝病和病毒性肝炎患者血清中检测率及其临床意义。方法 收集江阴市人民医院2010～2014年肝病患者血清,根据疾病诊断分为自身免疫性肝病组48例:包括自身免疫性肝炎(AIH)12例,原发性胆汁性肝硬化(PBC)36例; 病毒性肝炎组62例:包括10例HAV,30例HBV,14例HCV,8例HEV; 以及30例正常对照组。采用免疫印迹法分别检测上述血清标本中抗肝抗原(AMA-M2,LKM-1,LC-1,SLA/LP,GP210,SP100)自身抗体。结果 抗AMA-M2抗体,抗LKM-1抗体,抗LC-1抗体,抗SLA/LP抗体,抗GP210抗体和抗SP100抗体在AIH组的阳性率分别为16.7%(2/12),16.7%(2/12),8.3%(1/12),25%(3/12),0%(0/12)和16.7%(2/12); 在PBC组阳性率分别为83.3%(30/36),0%(0/36),0%(0/36),0%(0/36),44.4%(16/36)和27.8%(10/36); 在乙型肝炎患者中检出1例(3.3%,1/30)AMA-M2阳性,1例(3.3%,1/30)抗GP210抗体阳性,2例(6.7%,2/30)抗SP100抗体阳性; 在丙型肝炎患者中检出1例(7.1%,1/14)抗SP100抗体阳性; 在甲型肝炎、戊型肝炎未检出自身抗体。抗AMA-M2抗体、抗GP210抗体和抗SP100抗体在自身免疫性肝病组阳性率明显高于病毒性肝炎组(χ2值分别为33.9,10.6,8.8,P值均＜0.05)。抗SLA/LP抗体在AIH组阳性率明显高于PBC组(χ2=6.4,P＜0.05)。AMA-M2和抗GP210抗体在PBC组阳性率明显高于AIH组(χ2值分别为15.1和6.1,P＜0.05)。病毒性肝炎患者检出率低,与正常对照组比较差异无统计学意义(χ2=1.1,P＞0.05)。结论 抗肝抗体对自身免疫性肝病的明确诊断及鉴别诊断有重要的临床意义。
Abstract:
Objective To study the positive incidence and clinical significance of auto-antibody of liver antigens in the serum of patients with autoimmune hepatitis diseases and viral hepatitis.Methods The serum samples with hepatic diseases were collected in Jiangyin People's Hospital from 2010 to 2014 year.Patients were divided into three groups according to diseases:autoimmune hepatic disease group including autoimmune hepatitis(AIH)12 cases and primary biliary cirrhosis(PBC)36 case; viral hepaticgroup including HAV 23 cases,HBV 30 cases,HCV 14 cases and HEV 8 cases; normalcontrol group 30 cases.Auto-antibody of live antigens AMA-M2,LKM-1,LC-1,SLA/LP,GP210 and SP100 were tested respectively by western blotting assay.Results The positive rates of anti-AMA-M2,anti-LKM-1,anti-LC-1,anti-SLA/LP,anti-GP210,anti-SP100 were 16.7%,16.7%,8.3%,25%,0% and 16% respectively in AIH group,83.3%,0%,0%,0%,44.4% and 27.8%respectively in PBC group.Patients with viral hepatitis,one case were anti-AMA-M2 positive,one case were anti-GP210 positive and three case with positiveanti-SP100.anti-AMA-M2,anti-GP210 and anti-SP100 were detected more frequently in autoimmune liver diseases group than viral hepatitis group(χ2=33.9,10.6 and 8.8,P＜0.05),anti-SLA/LP were detected more frequentlyin AIH group than PBC group(χ2=6.4,P＜0.05),while anti-AMA-M2,anti-GP210 and were detected more frequently in PBC group than AIH group(χ2=15.1 and 6.1,P＜0.05).The positive rate of viral hepatic group was a very low frequency,there are little statistically significant difference(χ2=1.1,P＞0.05)compared with normal control group.Conclusion The antibody of liver antigens are useful clinically for diagnosing andclassifying autoimmune liver disease.

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备注/Memo

备注/Memo:
作者简介:林 江(1977-),女,硕士研究生,主管技师,研究方向:免疫检验,Tel:13812135610,E-mail:15086265@qq.com。
更新日期/Last Update: 2015-06-10