[1]沈守星,刘小凤,徐 刚,等.珠海地区性病实验室淋球菌培养鉴定能力室间质评结果分析[J].现代检验医学杂志,2015,30(03):159-161.[doi:10.3969/j.issn.1671-7414.2015.03.049]
　SHEN Shou-xing,LIU Xiao-feng,XU Gang,et al.Analisis of External Quality Assessment of Neisseria Gonorrhoeae Identification and Validation in STD Laboratories of Zhuhai Area[J].Journal of Modern Laboratory Medicine,2015,30(03):159-161.[doi:10.3969/j.issn.1671-7414.2015.03.049]
点击复制

珠海地区性病实验室淋球菌培养鉴定能力室间质评结果分析()
分享到：

《现代检验医学杂志》[ISSN:/CN:]

卷:
第30卷
期数:
2015年03期
页码:
159-161
栏目:
质量控制·实验室管理
出版日期:
2015-06-10

文章信息/Info

Title:
Analisis of External Quality Assessment of Neisseria Gonorrhoeae Identification and Validation in STD Laboratories of Zhuhai Area
文章编号:
1671-7414(2015)03-159-03
作者:
沈守星; 刘小凤; 徐 刚; 魏秋姣
珠海市慢性病防治中心,广东珠海 519000
Author(s):
SHEN Shou-xing; LIU Xiao-feng; XU Gang; WEI Qiu-jiao
the Center for Chronic Disease Control of Zhuhai,Guangdong Zhuhai 519000,China
关键词:
性病实验室;  淋球菌;  室间质评
分类号:
R378.16; R446
DOI:
10.3969/j.issn.1671-7414.2015.03.049
文献标志码:
A
摘要:
目的 为了建立珠海地区淋球菌耐药检测网络,了解性病实验室淋球菌分离鉴定的能力,及时发现影响鉴定结果的因素和存在的问题。方法2011～2014年度淋球菌质控样品统一发放到参评实验室,各室按照常规操作进行淋球菌培养分离鉴定和PPNG实验,各实验室在规定时间内回报鉴定结果,由市慢性病防治中心对回报结果进行统计分析,并将考评结果反馈给各参评实验室。结果 四年间共发放220份质评样本,综合考核成绩平均合格率88.6%(39/44); 淋球菌鉴定结果符合率分别为78.8%(26/33),90.3%(28/31),87.9%(29/33)和100%(33/33); PPNG符合率分别为80.8%(21/26),78.6%(22/28),86.2%(25/29)和100%(33/33)。结论 通过规范化建设性病实验室,每年进行室间质评活动,珠海地区性病实验室淋球菌培养鉴定能力明显提高,但个别实验室存在人员粗心大意、结果报告准确性和规范性等问题,仍需加强实验室质量控制工作。
Abstract:
Objective To recognize the ability to identifyand validate N.gonorrhoeae in Zhuhai STD laboratories to timely find out the factors or problems influencing the results.Methods Samples were uniformly delivered to STD laboratories in 2011～2014.The laboratories identified and validated N.gonorrhoeae and PPNG.Test results were analyzed and evaluation results were fed back to participating laboratories.Results 220 samples were delivered to STD laboratories in four years.The average percentage of qualified labs was 88.6%(39/44).The coincidence rates for N.gonorrhoeae quality control samples respectively were 78.8%(26/33),90.3%(28/31),87.9%(29/33)and 100%(33/33)in 2011～2014.The coincidence rates for PPNG respectively were 80.8%(21/26),78.6%(22/28),86.2%(25/29)and 100%(33/33).Conclusion Through the construction of STD standardization laboratory and the external quality assessment everyyear,the STD laboratories of Zhuhai area improved the ability to validate andidentify N.gonorrhoeae obviously.But several laboratories had some problem,such as careless technicians and inaccurate reports.So quality control shouldbe still strengthened.

参考文献/References:

[1] 尹跃平.性传播疾病实验室诊断指南[M].上海:科技出版社,2007:22.
Yin YP.Guidelines for Laboratory Diagnosis of Sexually TransmittedDiseases[M].Shanghai:Shanghai Scientific and Technology Press,2007:22.
[2] 中华人民共和国卫生部.WS268-2007淋病诊断标准[S].北京:人民卫生出版社,2009.
Department of Health poling and Pegulation,mimistry of Health.WS268-2007 Diagnostic Criteria for Gonorrhea[S].Beijing:People's Medical Publishing House,2009.
[3] 戴秀芹,尹跃平,彭锐锐,等.2010年全国性病实验室淋球菌分离鉴定室间质评结果分析[J].中华检验医学杂志,2011,34(6):551-553.
Dai XQ,Yin YP,Peng RR,et al.The analysis of external quality assessment of Neisseria Gonorrhoeae identification and validation in nationwideSTD laboratories in 2010[J].Chin J Lab Med,2011,34(6):551-553.
[4] 刘小凤,沈守星,魏秋姣,等.某地区淋球菌对抗菌药物敏感性及质粒耐药流行状况研究[J].检验医学与临床,2014,11(11):1530-1531,1534.
Liu XF,Shen SX,Wei QJ,et al.In vitro susceptibility to antimicrobial agents and resistance plasmids genotype of neisseria gonorrhoeae in Zhuhai area[J].Laboratory Medicine and Clinical,2014,11(11):1530-1531,1534.
[5] 曾维英,郑和平,黄进梅.广东省性病实验室淋球菌培养鉴定能力分析[J].热带医学杂志,2008,8(10):1069-1070.
Zeng WY,Zheng HP,Huang JM.Quality evaluation and proficiency testing of isolation and identification of neisseria gonorrhoear among the STIslaboratories in Guangdong[J].Journal of Tropical Medicine,2008,8(10):1069-1070.

备注/Memo

备注/Memo:
基金项目:珠海市医学科研基金项目(项目编号:2015J026)。
作者简介:沈守星(1980-),男,硕士,主管检验师,从事性病检验和实验室管理工作,研究方向为淋球菌耐药性检测及分子流行病学研究,Tel:13680361657,E-mail:649708763@qq.com。
更新日期/Last Update: 2015-06-10