[1]李大鹏a,吕春雷b,王凤娇a,等.大鼠X射线辐照后对血清抗氧化酶影响的实验研究[J].现代检验医学杂志,2015,30(06):94-97.[doi:10.3969/j.issn.1671-7414.2015.06.028]
 LI Da-penga,L Chun-leib,WANG Feng-jiaoa,et al.Experimental Study on Influence on the Antioxidant Enzymes of Serum in Rates Irradiated by X-Rays[J].Journal of Modern Laboratory Medicine,2015,30(06):94-97.[doi:10.3969/j.issn.1671-7414.2015.06.028]
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大鼠X射线辐照后对血清抗氧化酶影响的实验研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第30卷
期数:
2015年06期
页码:
94-97
栏目:
研究简报·实验技术
出版日期:
2015-12-30

文章信息/Info

Title:
Experimental Study on Influence on the Antioxidant Enzymes of Serum in Rates Irradiated by X-Rays
作者:
李大鹏a吕春雷b王凤娇a翟真真a郑文哲b
解放军88医院a.中心实验室;b.医务处,山东泰安271000
Author(s):
LI Da-pengaL Chun-leibWANG Feng-jiaoaZHAI Zhen-zhenaZHENG Wen-zheb
a.Department of Central Laboratory; b.Department of Medical,the 88th Hospital of PLA,Shandong Taian 271000,China
关键词:
X射线辐射大鼠脂质过氧化抗氧化酶超氧化物歧化酶过氧化氢酶谷胱甘肽过氧化物酶
分类号:
R-332
DOI:
10.3969/j.issn.1671-7414.2015.06.028
文献标志码:
A
摘要:
目的探讨大鼠X射线辐照后对血清抗氧化酶的影响。方法选择无特定病原体成年大鼠19只,随机分为试验组(A)、试验组(B)和对照组(C)。A,B两组大鼠均每日进行全身一次性X线照射,连续6天,1次/天。A组照射剂量为3Gy,B组照射剂量为2Gy,C组不进行X线照射,完毕后留取血样检测3组大鼠血清中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)活性,并予以统计分析。结果SOD:A组185.486±27.606 U/ml,B组255.087±43.519 U/ml,C组299.171±26.890 U/ml,A,C组间与A,B组间差异均有统计学意义(P<0.05),B与C组间差异无统计学意义(P>0.05),表明A组SOD水平显著低于C组,且A组显著低于B组;CAT:A组0.438±0.400 U/ml,B组1.173±0.457 U/ml,C组1.497±0.702 U/ml,A,C组间与A,B组间差异均有统计学意义(P<0.05),B与C组间差异无统计学意义(P>0.05),表明A组CAT水平显著低于C组,且A组显著低于B组;GSH-Px:A组3139.474±435.677 U/ml,B组3160.150±335.989 U/ml,C组3189.473±338.204 U/ml,GSH-Px检测各组间差异均无统计学意义(P>0.05),表明A和B组GSH-Px水平低于C组,且A组低于B组。结论X线辐射对血清SOD和CAT指标具有显著性影响,可显著降低血清SOD及CAT水平,抗氧化酶SOD及CAT活性与照射剂量呈负相关;X线辐射可降低血清GSH-Px水平,但对GSH-Px指标无显著性影响。
Abstract:
ObjectiveTo explore influence on the antioxidant enzymes of serum in rates irradiated by X-ray.MethodsChose 19 rats without specific pathogen free and they were randomly divided into experimental group (A),experimental group (B) and the control group (C).A,B two group rats were daily with whole body one-time X-ray irradiation,A,B group to X-ray irradiation for 6 d consecutive time and 1 time/d,A group radiation dose were 3Gy,B group radiation dose were 2Gy,C group hadn’t radiation.Then got blood specimens of 3 group rats and superoxide dismutase (SOD),hydrogen peroxide enzyme (CAT) and glutathione oxidation reductase (GSH-Px) wrer detected.The data had statistics analyse.ResultsSOD:A group 185.486±27.606 U/ml,B group 255.087±43.519 U/ml,C group 299.171±26.890 U/ml,the difference between A and C group was statistically significant (P<0.05),the difference between A and B group was statistically significant (P<0.05),no difference between B and C groups was statistically significant (P>0.05),while the level of SOD for A group was significantly below than C group,and A group was significantly below than B group.CAT:A group 0.438±0.400 U/ml,B group 1.173±0.457 U/ml,C group 1.497±0.702 U/ml,the difference between A and C group was statistically significant (P<0.05),the difference between A and B group was statistically significant (P<0.05),no difference between B and C groups was statistically significant (P>0.05),while the level of CAT for A group was significantly below than C group,and A group was significantly below than B group.GSH-Px:A group 3 139.474±435.677 U/ml,B group 3 160.150±335.989 U/ml,C group 3 189.473±338.204 U/ml,there was no statistically significant difference between the groups in GSH-Px detection (P>0.05),while the level of GSH-Px for A and B group were below than C group,and A group was below than B group.ConclusionX-ray radiation had significant influence on the SOD and CAT of serum,and could significantly reduce the level of serum SOD and CAT.The antioxidant enzyme active of SOD and CAT was anticorrelation with irradiation dose.X-ray radiation can reduce the level of serum GSH-Px,there was no significant influence on the GSH-Px.

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备注/Memo

备注/Memo:
作者简介:李大鹏(1964-),男,本科,副主任技师,研究方向:医学实验,Tel:13583848186,E-mail:lidapeng1964@126.com。 通讯作者:吕春雷,男,主任医师,Tel:15505486678,E-mail:15653808901@163.com。
更新日期/Last Update: 1900-01-01