[1]聂署萍,张 扬,王 琼,等.红霉素体外诱导金黄色葡萄球菌耐药分析[J].现代检验医学杂志,2016,31(01):138-139,143.[doi:10.3969/j.issn.1671-7414.2016.01.040]
　NIE Shu-ping,ZHANG Yang,WANG Qiong,et al.Drug-Resistance of Staphylococcus Aureus Induced by Erythromycin in Vitro[J].Journal of Modern Laboratory Medicine,2016,31(01):138-139,143.[doi:10.3969/j.issn.1671-7414.2016.01.040]
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红霉素体外诱导金黄色葡萄球菌耐药分析()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第31卷
期数:
2016年01期
页码:
138-139,143
栏目:
研究简报·实验技术
出版日期:
2016-01-25

文章信息/Info

Title:
Drug-Resistance of Staphylococcus Aureus Induced by Erythromycin in Vitro
作者:
聂署萍1; 张 扬1; 2; 王 琼1; 黄 烈1; 吴润香1; 凌利芬1; 范菲楠1
1.广东医学院附属深圳福田医院检验科,广东深圳 518033; 2.陕西中医学院,陕西咸阳 712046
Author(s):
NIE Shu-ping1; ZHANG Yang1; 2; WANG Qiong1; HUANG Lie1; WU Run-xiang1;  LING Li-fen1; FAN Fei-nan1
1.Department of Laboratory Medicine,Futian Hospital of Guangdong Medical College,Guangdong Shenzhen 518033,China; 2.Shaanxi University of Chinese Medicine,Shaanxi Xianyang 712046,China
关键词:
金黄色葡萄球菌;  红霉素;  交叉耐药性
分类号:
R378.11; R446.5
DOI:
10.3969/j.issn.1671-7414.2016.01.040
文献标志码:
A
摘要:
目的 体外诱导金黄色葡萄球菌标准株ATCC25923对红霉素耐药,分析其耐药后菌株生长及对其它药物敏感性变化。方法 用红霉素浓度2倍递增的方法对金黄色葡萄球菌ATCC25923进行体外诱导,比较诱导前后金黄色葡萄球菌的生长表型以及对其它抗菌药物的耐药性变化。结果 诱导后ATCC25923红霉素MIC由0.032 5 mg/L增加到>256 mg/L,诱导耐药后金黄色葡萄球菌出现生长缓慢,溶血性减弱等变异,对其它非诱导抗菌药物耐药性未发生变化。结论经红霉素体外诱导后金黄色葡萄球菌可以获得稳定耐药性,但同时也产生了表型及部分生化特性变化,与其它非诱导抗菌药物无交叉耐药。
Abstract:
Objective To investigate the drug-resistance of Staphylococcus aureus induced by erythromycin in vitro and its changes ingrowth and susceptibility of antibiotics.Methods Erythromycin in vitro induction was conducted with the S.aureus reference strainATCC25923 on the serial of erythromycin agar plates.The growth of S.aureus,and the susceptibility against other antibiotics was compared after induced tothe parent strain.Results Resistance to erythromycin was successfully,and themaximum MIC was over 256 mg/L.The Erythromycin-resistant S.aureus grew much slower than the susceptible parent,and the strains didn't have cross-resistance to other antibiotics.Conclusion S.aureus could be induced resistance in vitro by erythromycin,and this resistance inherited stably.Some phenotype and biochemical characteristic features of the strain were changed after induced.

参考文献/References:

[1] Yang Z,Wang J,Wang W,et al.Proportions of Sta-phylococcusaureus and methicillin-resistant Staphylococcus aureus in patients with surgical site infections in mainland China:a systematic review and meta-analysis[J].PLoS One,2015,10(1):e0116079.
[2] Gardiner BJ,Grayson ML,Wood GM.Inducible resistance to clindamycin inStaphylococcus aureus:validation of Vitek-2 against CLSI D-test[J].Pathology,2013,45(2):181-184.
[3] 陈方圆,马笑雪,蔡景钰,等.多重耐药性金黄色葡萄球菌(MRSA)的临床药物治疗及耐药机制研究[J].微生物学杂志,2010,30(1):71-74. Chen FY,Ma XX,Cai JY,et al.Clinical medical treatment against multiple drug-resistant Staphylococcus aureus(MDRSA)and its drug-resistancemechanism[J].Journal of Microbiology,2010,30(1):71-74.
[4] 郝秀红,赵强元,李艳君,等.2008年～2011年金黄色葡萄球菌的临床分布和耐药特征[J].现代检验医学杂志,2013,28(1):59-62. Hao XH,Zhao QY,Li YJ,et al.Clinical distribution and drug resistance of Staphylococcus aureus to antibiotics in recent four years[J].Journal of Modern Laboratory Medicine,2013,28(1):59-62.
[5] 李艳芬,王 俊,王淑香,等.利奈唑胺体外诱导金黄色葡萄球菌耐药的实验研究[J].中华实验和临床感染病杂志(电子版),2013,7(5):694-698. Li YF,Wang J,Wang SX,et al.The drug-resistance of Staphylococcus aureus induced by linezolid in vitro[J].Chinese Journal of Experimentaland Clinical Infectious Diseases(Electronic Edition),2013,7(5):694-698.
[6] 王 强,王 跃,刘明方.红霉素体外诱导肺炎链球菌耐药的研究[J].第三军医大学学报,2009,31(12),1185-1188. Wang Q,Wang Y,Liu MF.Study on the Streptococcus pneumoniae resistance induced by erythromycin in vitro[J].Acta Academiae Medicinae Militaris Tertiae,2009,31(12),1185-1188.
[7] 王 轶,刘珊珊,黄 瑾.红霉素诱导呼吸道分离金黄色葡萄球菌耐药状况研究[J].中国实验诊断学,2011,15(10):1719-1720. Wang Y,Liu SS,Huang J.Study on Staphylococcus aureus from respiratory tract resistance induced by erythromycin[J].Chinese Journal of Laboratory Diagnosis,2011,15(10):1719-1720.

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备注/Memo

备注/Memo:
基金项目:深圳市福田区卫生系统公益性科研项目(FTWS201236)。 作者简介:聂署萍(1983-),女,硕士,副主任技师,从事微生物鉴定与耐药监测研究,E-mail:nieping2@126.com。
更新日期/Last Update: 2016-01-20