[1]李 娅,张 赟,皇 海,等.HCV-RNA与HCV-Ab,HCV-cAg相关性分析研究[J].现代检验医学杂志,2016,31(05):120-122.[doi:10.3969/j.issn.1671-7414.2016.05.034]
　LI Ya,ZHANG Yun,HUANG Hai,et al.Correlation Analysis of HCV-RNA,HCV-Ab and HCV-cAg[J].Journal of Modern Laboratory Medicine,2016,31(05):120-122.[doi:10.3969/j.issn.1671-7414.2016.05.034]
点击复制

HCV-RNA与HCV-Ab,HCV-cAg相关性分析研究()
分享到：

《现代检验医学杂志》[ISSN:/CN:]

卷:
第31卷
期数:
2016年05期
页码:
120-122
栏目:
研究简报·实验技术
出版日期:
2016-10-21

文章信息/Info

Title:
Correlation Analysis of HCV-RNA,HCV-Ab and HCV-cAg
文章编号:
1671-7414(2016)05-120-03
作者:
李 娅1; 张 赟1; 皇 海2; 章 迪3; 苏明权2; 郭旭昌1
1.西安市第四医院检验科,西安 710004;
2.第四军医大学西京医院全军检验医学研究所,西安 710032;
3.西安市中心血站,西安 710061
Author(s):
LI Ya1; ZHANG Yun1; HUANG Hai2; ZHANG Di3; SU Ming-quan2; GUO Xu-chang1
1.Department of Clinical Laboratory,the Fourth Hospital of Xi'an,Xi'an 710004,China;
2.Research Institute of Clinical Laboratory Medicine of PLA,Xijing Hospital,the Fourth Military Medical University,Xi'an 710032,China;
3.Blood Center of Xi'an,
关键词:
丙型肝炎病毒;  丙型肝炎核酸;  丙型肝炎病毒核心抗原;  丙型肝炎病毒抗体
分类号:
R512.63; R392.11
DOI:
10.3969/j.issn.1671-7414.2016.05.034
文献标志码:
A
摘要:
目的 探讨丙型肝炎患者中HCV-RNA与HCV-Ab,HCV-cAg三种检测指标的相关性和临床应用效果。方法 采用酶联免疫法分别检测实时荧光定量PCR法检测阳性的140例HCV患者血清中的HCV-Ab和HCV-cAg,以了解检测结果及符合率。结果 在140例HCV-RNA阳性血清中,HCV-cAg阳性127例,符合率90.71%; HCV-Ab阳性110例,符合率78.57%; 对不同HCV-RNA浓度的血清进行HCV-cAg检测结果其阳性检出率随着HCV病毒含量的升高而增高; 不同HCV-RNA浓度的血清进行HCV-Ab检测结果无明显变化。结论 通过对HCV-RNA阳性患者中HCV-Ag和HCV-Ab的检测观察分析,HCV-cAg检测与HCV-RNA的检测结果具有较高的符合率。因此HCV-cAg检测可作为HCV-Ab的补充检测,为HCV“窗口期”感染以及免疫低下人群感染的筛查提供简便、廉价方法; 同时为不具备HCV-RNA检测条件的基层医疗单位,作为HCV感染的快速筛查提供诊断依据。
Abstract:
Objective To investigate the correlation of HCV-RNA with detection indexes HCV-Ab and HCV-cAg in its clinical application effect among patients with hepatitis C.Methods HCV-cAg and HCV-Ab in 140 cases of HCV-RNA were detected by enzyme linked immunosorbent assay in cases of PCR,which were detected by real-time fluorescence quantitative PCR.Results 127 cases in 140 cases of HCV-RNA positive serum were HCV-cAg positive,in line with the rate of 90.71%,and the cases of 110 HCV-Ab positive,in line with the rate of 78.57%.The positive detection rate of HCV-cAg with different HCV-RNA concentration was increased with the increase of HCV virus content,and the serum of different HCV-RNA concentration had no significant changes in HCV-Ab detection results.Conclusion The detection results of HCV-cAg had a high coincidence rate with HCV-RNA.Therefore detection of HCV-cAg can be as a complementary detection of HCV-Ab,as the window period of HCV infection and infection in immunocompromised persons screening provides a simple,inexpensive method.At the same time it provides rapid screening for HCV infection provide diagnostic basis for those basic medical units who do not have the conditions for detection of HCV-RNA.

参考文献/References:

[1] 许 方,李晓兰,祝 琳.丙肝病毒核心抗原检测对于丙型肝炎诊断的价值[J].中国实验诊断学,2010,14(5):713-715. Xu,Li XL,Zhu L.Diagnostic value of HCV core antigen in the hepatitis C[J].Chinese Jouurnal of Laboratory Diagnostics,2010,14(5):713-715.
[2] 张 瑞,李金明.丙型肝炎病毒感染临床检测程序的建立及结果报告与解释[J].中华检验医学杂志,2010,33(10):990-992. Zhang R,Li JM.Laboratory testing and result reporting of antibodyto hepatitis C virus[J].Chinese Journal of Laboratory Medicine,2010,33(10):990-992.
[3] 李 蓉.丙肝抗HCV检测与荧光定量PCR HCV-RNA检测结果对比分析[J].临床医学杂志,2015,35(5):110-111. Li R.Anti HCV HCV detection with fluorescence quantitative PCR,comparative analysis of the results of HCV-RNA[J].Journal of Clinical Medicine,2015,35(5):110-111.
[4] 曹军皓,黄前川.丙肝病毒核心总抗原定量检测的临床应用[J].现代检验医学杂志,2014,29(2):108-109,114. Cao JH,Huang QC.Clinical application and evaluation of hepatitis Cvirus core antigen quantitative assays[J].Journal of Modern Laboratory Medicine,2014,29(2):108-109,114.
[5] Bostan N,Mahmood T.An overview about hepatitis C:Adevasting virus[J].Crit Rew Microbial,2010,36(2):91-133.
[6] Lavanchy D.Evoling epidemiology of hepatitis C vi-rus[J].Clin Microbiol Infect,2011,17(2):107-115.

相似文献/References:

[1]吴泽刚,李 艳,郑红云.湖北地区慢性丙型肝炎患者HCV基因分型结果分析[J].现代检验医学杂志,2016,31(01):38.[doi:10.3969/j.issn.1671-7414.2016.01.010]
　WU Ze-gang,LI Yan,ZHENG Hong-yun.Analysis on HCV Genotype of Patients with Chronic Hepatitis C inHubei[J].Journal of Modern Laboratory Medicine,2016,31(05):38.[doi:10.3969/j.issn.1671-7414.2016.01.010]
[2]甘志超,刘 莹,赵冰红,等.丙型肝炎患者自身抗体检出率、 丙肝病毒载量及肝功能间的相关性[J].现代检验医学杂志,2016,31(03):108.[doi:10.3969/j.issn.1671-7414.2016.03.030]
　GAN Zhi-chao,LIU Ying,ZHAO Bing-hong,et al.Correlation among the Positive Rate of Autoantibody,HCV Load and the Status of Liver Function in Patients Infected HCV[J].Journal of Modern Laboratory Medicine,2016,31(05):108.[doi:10.3969/j.issn.1671-7414.2016.03.030]
[3]蒋玲丽,杨依绡,黄中强,等.上海地区临床实验室HCV RNA检测正确度验证结果分析[J].现代检验医学杂志,2019,34(05):156.[doi:10.3969/j.issn.1671-7414.2019.05.040]
　JIANG Ling-li,YANG Yi-xiao,HUANG Zhong-qiang,et al.Analysis on the Results of HCV RNA Determination Trueness Verification of Clinical Laboratories in Shanghai[J].Journal of Modern Laboratory Medicine,2019,34(05):156.[doi:10.3969/j.issn.1671-7414.2019.05.040]
[4]李雅静,王戌刚,董倩倩,等.河北地区丙肝感染患者丙型肝炎病毒基因型分布特征[J].现代检验医学杂志,2020,35(06):87.[doi:doi:10.3969/j.issn.1671-7414.2020.06.021]
　LI Ya-jing,WANG Xu-gang,DONG Qian-qian,et al.HCV Genotype and Subtype Distribution of Patients with ChronicHepatitis C in Hebei Area[J].Journal of Modern Laboratory Medicine,2020,35(05):87.[doi:doi:10.3969/j.issn.1671-7414.2020.06.021]
[5]冯飞雪,王大望,史小武,等.DA3200-ABI7500全自动核酸提取平台高敏 HCV…RNA定量检测性能验证[J].现代检验医学杂志,2021,36(01):108.[doi:10.3969/j.issn.1671-7414.2021.01.027]
　FENG Fei-xue,WANG Da-wang,SHI Xiao-wu,et al.Performance Verification of DA3200-ABI7500 Automatic Nucleic Acid Extraction Platform for High Sensitive HCV RNA Quantitative Detection[J].Journal of Modern Laboratory Medicine,2021,36(05):108.[doi:10.3969/j.issn.1671-7414.2021.01.027]

备注/Memo

备注/Memo:

作者简介:李 娅(1980-),女,硕士研究生,主管检验师,研究方向:分子生物学,Tel:029-87480873,E-mail:yaya50211cm@sina.com。
更新日期/Last Update: 2016-10-30