[1]洪 俊,饶永彩.评估CellaVisionTM DM96全自动数字细胞形态学分析系统在外周血血涂片中检测细胞内寄生虫的临床价值[J].现代检验医学杂志,2016,31(05):123-126，129.[doi:10.3969/j.issn.1671-7414.2016.05.035]
　HONG Jun,RAO Yong-cai.Performance Evaluation of the CellaVisionTM DM96 AnalyzerDuring Detection of Intracellular Parasites of Peripheral Blood Smears[J].Journal of Modern Laboratory Medicine,2016,31(05):123-126，129.[doi:10.3969/j.issn.1671-7414.2016.05.035]
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评估CellaVisionTM DM96全自动数字细胞形态学分析系统在外周血血涂片中检测细胞内寄生虫的临床价值()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第31卷
期数:
2016年05期
页码:
123-126，129
栏目:
研究简报·实验技术
出版日期:
2016-10-21

文章信息/Info

Title:
Performance Evaluation of the CellaVisionTM DM96 AnalyzerDuring Detection of Intracellular Parasites of Peripheral Blood Smears
文章编号:
1671-7414(2016)05-123-05
作者:
洪 俊1; 饶永彩2
1.武汉大学人民医院检验科,武汉 430060;
2.武汉生物制品所,武汉 430060
Author(s):
HONG Jun1; RAO Yong-cai2
1.Departmentof Clinical Laboratory,Renmin Hospital of Wuhan University,Wuhan 430060,China;
2.Wuhan Institute of Biological Products,Wuhan 430060,China
关键词:
血涂片;  细胞内寄生虫;  CellaVisionTM DM96全自动数字细胞形态学分析系统
分类号:
R446.111
DOI:
10.3969/j.issn.1671-7414.2016.05.035
文献标志码:
A
摘要:
目的 评估全自动数字细胞形态学分析系统CellaVisionTMDM96在外周血血涂片中检测细胞内寄生虫的能力。方法 检验普通技师和血液专业技师分别采用光学显微镜和CellaVisionTM DM96对142张阳性血涂片和165张阴性对照组血涂片进行判读,然后对判读结果进行统计学分析。对阳性血涂片按疟原虫含量百分率进行分类,观察CellaVisionTMDM96对不同含量疟原虫血涂片的疟原虫检出率。结果 检验普通技师和血液专业技师无论是使用光学显微镜还是CellaVisionTM DM96都能准确识别所有阴性对照,其特异性均为100%。血液专业技师使用CellaVisionTM DM96的敏感度与实验室普通技师和血液专业技师使用光学显微镜检测疟原虫的敏感度均无统计学差异(χ2=0.24,0.03,0.43,P>0.05)。血涂片中疟原虫百分率越高,CellaVisionTM DM96的疟原虫检出率就越高。当血涂片疟原虫百分率≥1%时,仅有2个含有恶性疟原虫的样本被误判为阴性。血涂片疟原虫百分率≥2.0%时使用CellaVisionTM DM96能将所有血涂片100%正确判读。结论 CellaVisionTM DM9检测疟原虫的特异度和敏感度与光学显微镜一致。使用CellaVisionTM DM9检测疟原虫提供了另一种检测红细胞内疟原虫的方法。
Abstract:
Objective To evaluate the performance of the CellaVisionTM DM96 analyzer during detection of intracellular parasites ofperipheral blood smears.Methods This study compared thedetection rates of Plasmodium and Babesia species on peripheral blood smears utilizing the CellaVision DM96 with the rates for a routine red blood cell morphology scan.A total of 307 slides were analyzed,consisting of 142 slides positivefor Plasmodium or Babesia species and 165 negative controls.Slides were blinded,randomized,and analyzed by CellaVision 96 and microscopy for red cell morphology scans.The technologists were blinded to prior identification.Results The examination of common technician and blood professional technician,whether using optical microscope or CellaVision DM96 could accuratelyidentify all the negative control,its specificity was 100%.The sensitivity ofblood professionals using CellaVision DM96 was not statistically significant inthe sensitivity of the laboratory and the use of light microscopy for the detection of Plasmodium parasites(χ2=0.24,0.03,0.43,P>0.05).The higher percentage of Plasmodium in blood smear,the higher detection rate of CellaVision DM96.When the blood smear Plasmodium white rate greater than 1%,only 2contain Plasmodium falciparum samples were negative for the miscarriage of justice.The blood smear percentage was higher than 2% CellaVision of Plasmodium DM96 will all the blood smear 100% correct interpretation.ConclusionThis study demonstrates that CellaVision had the potential to detectintracellular parasites in routine screening of blood smears resource for storing images to be used for educational purposes,including training technologists in the detection of intracellular parasites.

参考文献/References:

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备注/Memo

备注/Memo:

作者简介:洪 俊(1973-),男,博士,副主任技师,主要从事临床免疫及血液学方面的研究,Tel:15971431905,E-mail:wonderman1973@hotmail.com。
更新日期/Last Update: 2016-10-30