[1]郝树彬,孙洪厚,孙秀会,等.氧化酶法和钒酸盐法测定血清胆红素的方法比较[J].现代检验医学杂志,2017,32(01):113-116,120.[doi:10.3969/j.issn.1671-7414.2017.01.031]
　HAO Shu-bin,SUN Hong-hou,SUN Xiu-hui,et al.Developed for the Determination of Serum Bilirubin between Oxidase Method and Vanadate Oxidation Method[J].Journal of Modern Laboratory Medicine,2017,32(01):113-116,120.[doi:10.3969/j.issn.1671-7414.2017.01.031]
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氧化酶法和钒酸盐法测定血清胆红素的方法比较()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第32卷
期数:
2017年01期
页码:
113-116,120
栏目:
研究简报·实验技术
出版日期:
2017-01-25

文章信息/Info

Title:
Developed for the Determination of Serum Bilirubin between Oxidase Method and Vanadate Oxidation Method
文章编号:
1671-7414(2017)01-113-05
作者:
郝树彬1; 孙洪厚2; 孙秀会3; 刘兴翠4; 王志全2; 孙爱国5
1.山东省医疗器械产品质量检验中心/山东省医疗器械生物学评价重点实验室,济南 250101;
2.莘县中日友好医院,山东聊城 252400;
3.潍坊泽城生物技术有限公司,山东潍坊 261100;
4.山东省恒信检测技术开发中心,济南 250101;
5.青州荣军医院,山东潍坊 262500
Author(s):
HAO Shu-bin1; SUN Hong-hou2; SUN Xiu-hui3; LIU Xing-cui4; WANG Zhi-quan2; SUN Ai-guo5
1.Shandong Quality Inspection Center for Medical Devices/Shandong Medical Devices Biological Evaluation of Key Laboratory,Jinan 250101,China;
2.the China-Japan Friendship Hospital of Shen Country,Shandong Liaocheng 252400,China;
3.Weifang Zechen
关键词:
总胆红素;  直接胆红素;  胆红素氧化酶法;  钒酸盐氧化法;  新生儿直接胆红素
分类号:
R446.112
DOI:
10.3969/j.issn.1671-7414.2017.01.031
文献标志码:
A
摘要:
目的 通过对测定结果进行比对,研究胆红素钒酸盐氧化法和胆红素氧化酶法的反应方法原理,探讨两种方法之间异同点及结果是否具有可比性。方法 以两种方法分别检测310例血清,计算回归方程,评估两种方法的相关性。对异常样本进一步实验以研究方法之间的异同。结果 总胆红素测定结果的回归方程为Y= 1.065 1X+1.197 2,相关系数r=0.997 0。成年人及＞30天儿童直接胆红素测定结果回归方程为Y=0.945 9X+0.599 5,相关系数r=0.994 4; 新生儿测定结果的回归方程为Y=0.410 4X+2.756 3,相关系数r=0.883 5; 结果异常新生儿血清梯度稀释后钒酸盐氧化法结果不可接受。结论 总胆红素和成人及＞30天儿童的直接胆红素两种方法测定结果的相关性在可接受范围内; 新生儿直接胆红素测定两种方法的相关性不可接受; 钒酸盐氧化法不可用于新生儿直接胆红素测定。
Abstract:
Objective To study reaction principle of bilirubin vanadate oxidation method and bilirubin oxidase method through comparison of the determination results,and discuss similarities and difference between the two methods.Methods 310 cases were measured and analyzed with each method.Abnormal samples were further investigated.Results Fortotal bilirubin,the regression equation obtained was Y=1.065 1X+1.197 2,the correlation coefficient r=0.997 0.For direct bilirubin of adults and children greater than 30 days,the regression equation was Y=0.945 9X+0.599 5 and the correlation coefficient r=0.994 4.For neonatal direct bilirubin,the regression equation was Y=0.410 4X+2.756 3 and the correlation coefficient r=0.883 5.The results from vanadate oxidation method were unacceptable for abnormal neonatal serum measurement after serial dilution.Conclusion The overall conclusions were that for the measurement of total bilirubin,and direct bilirubin for adults and children older than 30 days.The correlation between thesetwo methods is in an acceptable range,for measurement of neonatal direct bilirubin,the correlation between thesetwo methods was not acceptable.It is not recommended to measure neonatal direct bilirubin by vanadate oxidation method.

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备注/Memo

备注/Memo:
作者简介:郝树彬(1979-),男,硕士研究生,研究方向:微生物学和体外诊断技术,Tel:15805317808,E-mail: 530082989@qq.com。
通讯作者:孙秀会,Tel:13655366231,E-mail:xinzebio@126.com。
更新日期/Last Update: 2017-01-20