[1]曹 云a,张晓艳a,曹德勇,等.凝血五项质控血浆低温冷冻后复融方式对其稳定性的影响[J].现代检验医学杂志,2017,32(01):128-130.[doi:10.3969/j.issn.1671-7414.2017.01.035]
　CAO Yuna,ZHANG Xiao-yana,CAO De-yong,et al.Thawing Modes on Stability of Coagulation Control Products afterFrozen[J].Journal of Modern Laboratory Medicine,2017,32(01):128-130.[doi:10.3969/j.issn.1671-7414.2017.01.035]
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凝血五项质控血浆低温冷冻后复融方式对其稳定性的影响()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第32卷
期数:
2017年01期
页码:
128-130
栏目:
研究简报·实验技术
出版日期:
2017-01-25

文章信息/Info

Title:
Thawing Modes on Stability of Coagulation Control Products afterFrozen
文章编号:
1671-7414(2017)01-128-03
作者:
曹 云1a; 张晓艳1a; 曹德勇2; 张 瑞3; 辛田田1b
1.延安市人民医院a.检验科;
b.重症医学科,陕西延安 716000;
2.延安大学附属医院东关分院心血管内科,陕西延安 716000;
3.延安大学附属医院检验科,陕西延安 716000
Author(s):
CAO Yun1a; ZHANG Xiao-yan1a; CAO De-yong2; ZHANG Rui3; XIN Tian-tian1b
1a.Department of Clinical Laboratory;
1b.Department ofICU,the People's Hospital of Yan'an, Shaanxi Yan'an 716000,China;
2.Department of Cardiovascular,Dongguan Branch of Yan'an University Hospital,Shaanxi Yan'an 716000,China;
3.Departmentof Cl
关键词:
凝血质控物;  复融;  稳定性
分类号:
R446.111
DOI:
10.3969/j.issn.1671-7414.2017.01.035
文献标志码:
A
摘要:
目的 探讨凝血五项质控血浆低温冷冻后复融方式对其稳定性的影响,为实验室成本控制寻找新的理论依据。方法 采用美国IL公司ACL TOP700全自动血凝分析仪及其配套同批号试剂和质控品进行该项研究:常规做完质控后,立即将剩余质控品分装到2个EP管中并冻存于-40℃,24 h后通过室温放置30 min和37℃水浴快速两种方式复融,并随当日质控物一起进行检测,连续收集20组数据分析两种复融方式对凝血系列质控品质控参数稳定性的影响。结果 室温复融组复融后测定的APTT,PT中值和高值,FIB中值,D-Dimer高值复融前后差异均无统计学意义(P＞0.05); 而FIB高值平均升高0.23 g/L(t=4.0269,P＜0.05); TT中值平均缩短0.46 s(t=-3.813 8,P＜0.05),TT高值平均缩短0.41s(t=-3.972 8,P＜0.05); D-Dimer低值平均增加14.75 ng/ml FEU(t=2.281 6,P＜0.05)。37℃水浴快速复融组复融后测定的APTT,PT,FIB,TT,D-Dimer两个水平差异均无统计学意义(P＞0.05)。结论 商用凝血质控品短期冻融后只要遵循快速复融原则并及时检测,是可以作为二次质控物来应用,其他实验室可作为参考。
Abstract:
Objective To investigate the thawing modes on stability of coagulation control products after frozen,looking for a new theoretical basis for cost control and the quality and safety of laboratory.Methods Using ACL TOP 700 automated coagulation analyzer and supporting the same batch of reagents and quality control materials conduct of the study:after daily QC,recycled the remaining control materials immediately and dispensed into two EP tubes and frozen at -40℃,respectively thawed by room-temperature and 37℃water bath after 24 hours, and examined together with the dateof quality control material,got 20 pairs of data for analysis the financial impact of two alternate ways on coagulation QC parameters.Results For the room-temperature thawing group,FIB high value increasedby an average 0.23 g/L(t=4.026 9,P＜0.05); TT normal value average reduction of 0.46 s(t=-3.813 8,P＜0.05),TT high value reduced by an average 0.41s(t=-3.972 8,P＜0.05); D-Dimer low-value increased by an average of 14.75 ng/ml FEU(t=2.281 6,P＜0.05),while APTT,PT normal and high value,FIB normal value,D-Dimer high value were no significant difference after thawing(P＞0.05).For the 37℃ water bath group,both normal and high valueof APTT,PT,FIB,TT and D-Dimer were no significant difference after thawing(P＞0.05).Conclusion The commercialization of coagulation control materials can be for the second QC,just follow the principle of rapid after melting and timely detection,other laboratories can be used as a reference.

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备注/Memo

备注/Memo:
基金项目:延安市科技惠民专项(2015HM-16)。
作者简介:曹 云(1964-),女,本科,主任检验师,研究方向:主要从事临床检验及质量控制方面的研究。
通讯作者:辛田田,主治医师,研究方向:重症患者凝血稳态变化机制,E-mail:yananznfe@126.com。
更新日期/Last Update: 2017-01-20