[1]李 勤,石 佳,卢兴兵,等.XN-9000全自动血液细胞分析仪有核红细胞计数与手工方法验证的探讨[J].现代检验医学杂志,2017,32(01):124-127.[doi:10.3969/j.issn.1671-7414.2017.01.034]
　LI Qin,SHI Jia,LU Xing-bing,et al.Evaluation of the Nucleated Red Blood Cells Count of Sysmex XN-9000 Automatic Hematology Analyzer and the Validation of Manual Method[J].Journal of Modern Laboratory Medicine,2017,32(01):124-127.[doi:10.3969/j.issn.1671-7414.2017.01.034]
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XN-9000全自动血液细胞分析仪有核红细胞计数与手工方法验证的探讨()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第32卷
期数:
2017年01期
页码:
124-127
栏目:
研究简报·实验技术
出版日期:
2017-01-25

文章信息/Info

Title:
Evaluation of the Nucleated Red Blood Cells Count of Sysmex XN-9000 Automatic Hematology Analyzer and the Validation of Manual Method
文章编号:
1671-7414(2017)01-124-04
作者:
李 勤; 石 佳; 卢兴兵; 毛志刚; 朱新勤; 江 虹; 曾素根
四川大学华西医院实验医学科,成都 610041
Author(s):
LI Qin; SHI Jia; LU Xing-bing; MAO Zhi-gang;  ZHU Xin-qin; JIANG Hong; ZENG Su-gen
Department of Clinical Laboratory, Huaxi Hospital of Sichuan University,Chengdu 610041,China
关键词:
血液细胞分析流水线;  有核红细胞计数;  手工验证
分类号:
R446.111
DOI:
10.3969/j.issn.1671-7414.2017.01.034
文献标志码:
A
摘要:
目的 探讨日本Sysmex公司生产的XN-9000全自动血液细胞分析流水线有核红细胞(nucleated red blood cell,NRBC)计数,与手工方法进行比较,验证仪器计数结果的准确性。方法 对60例全自动血液细胞分析流水线检测出NRBC大于1%的患者的血液标本,制作血液涂片染色后通过传统的显微镜手工方法镜检,验证仪器结果。结果 60例临床标本的NRBC与显微镜计数的结果比较,所有检测值的可信度分析均在可信范围内; 相关性比较中,NRBC(%)在1～10和＞10两组的仪器与手工方法的相关系数(r)分别为0.972 1和0.996 2,差异均无统计学意义(P＞0.05)。结论 无论NRBC值的高低,XN-9000全自动血液细胞分析流水线检测NRBC计数,与手工方法结果比较,均在可信范围内,相关性也较好。仪器检测的NRBC准确可靠,可应用于临床标本的检测。
Abstract:
Objective To evaluate the nucleated red blood cell(NRBC)count of Sysmex XN-9000 automatic hematology analysis lines comparing with manual method,and verify the accuracy of the analyzer results.Methods 60 blood samples with more than 1% of NRBCs detected by XN-9000 were counted NRBCs by traditional manual microscopy in blood smears,and verified the analyzer results.Results According tothe reliability analysis,the results of total 60 samples were all within the range of the reliability; correlation analysis showed that correlation coefficient(r)of group NRBC(%)1～10 and ＞10 were 0.972 1 and 0.996 2,respectively.There were no significant differences between them(P＞0.05).Conclusion Compared with manual method,the results of NRBC count ofXN-9000 were within the range of reliability,and showed good correlation.Theanalyzer test results of NRBC were accurate and reliable and could be applied tothe detection of clinical samples.

参考文献/References:

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备注/Memo

备注/Memo:
作者简介:李 勤(1989-),女,本科,检验技师,主要从事临床血液方面的研究,E-mail:476807260@qq.com。
通讯作者:曾素根,高级实验师,E-mail:zsg8077118@163.com。
江 虹,教授,Tel:028-80298017。
更新日期/Last Update: 2017-01-20