[1]张 磊,李 娟,王金华,等.实体肿瘤化疗患者使用粒细胞集落刺激因子对外周血中性粒细胞形态参数变化的影响[J].现代检验医学杂志,2017,32(06):128-131.[doi:10.3969/j.issn.1671-7414.2017.06.001]
　ZHANG Lei,LI Juan,WANG Jin-hua,et al.Changes of Neutrophil Morphological Parameters in Peripheral Blood of Patients with Solid Tumor Treated with Granulocyte Colony Stimulating Factor[J].Journal of Modern Laboratory Medicine,2017,32(06):128-131.[doi:10.3969/j.issn.1671-7414.2017.06.001]
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实体肿瘤化疗患者使用粒细胞集落刺激因子对外周血中性粒细胞形态参数变化的影响()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第32卷
期数:
2017年06期
页码:
128-131
栏目:
研究简报·实验技术
出版日期:
2017-12-25

文章信息/Info

Title:
Changes of Neutrophil Morphological Parameters in Peripheral Blood of Patients with Solid Tumor Treated with Granulocyte Colony Stimulating Factor
文章编号:
1671-7414(2017)06-128-04
作者:
张 磊1; 李 娟2; 王金华1; 张彦平1; 黄 伦1; 张 雯1
1.西安交通大学第二附属医院检验科,西安 710004; 2.希森美康医用电子(上海)有限公司,上海 200120
Author(s):
ZHANG Lei1; LI Juan2; WANG Jin-hua1; ZHANG Yan-pin1; HUANG Lun1; ZHANG Wen1
1.Department of Clinical Laboratory,the Second Affiliated Hospital of Xi'an Jiaotong University,Xi'an 71004,China; 2.Medical Electronics(Sysmex)CO.Shanghai Ltd,Shanghai 200120,China
关键词:
实体肿瘤;  化疗;  中性粒细胞胞内颗粒度;  粒细胞集落刺激因子;  外周血白细胞形态参数
分类号:
R730.43
DOI:
10.3969/j.issn.1671-7414.2017.06.001
文献标志码:
A
摘要:
目的 比较实体肿瘤化疗患者使用粒细胞集落刺激因子(G-CSF)前后外周血中性粒细胞侧向散射光强度即颗粒度(neutrophil lateral scattering intensity,NEUT-X)的变化并探讨其临床意义。方法 选取52例肿瘤化疗使用G-CSF患者(研究组),32例肿瘤化疗未使用G-CSF患者(研究对照组)和50例门诊表观健康查体者(健康对照组),使用Sysmex XE-2100全自动血细胞分析仪检测研究对象的外周血细胞计数,收集外周血白细胞形态参数结果数据。分析研究NEUT-X参数在化疗过程中的变化,同时观察三组患者临床感染发热率,以揭示白细胞形态参数与机体抵抗能力的关系。结果 研究组、研究对照组和健康对照组外周血中性粒细胞NEUT-X为:1 324(890.2,1 358.0),1 440(1 397.3,1 466.3),1 329(1 295.1,1 359.4),三组之间差异具有统计学意义(F=10.778,P=0.002),且两两比较差异具有统计学意义; 研究组患者使用G-CSF前后白细胞数分别为:0.99(0.22,1.75)×109/L,7.53(1.00,14.05)×109/L,两者之间差异具有统计学意义(Z=-2.395,P=0.005); 研究组患者使用G-CSF前后中性粒细胞形态参数NEUT-X分别为:1 382(1 323.6,1 440.4),1 324(890.2,1 358.0),两者之间差异具有统计学意义(Z=-2.832,P=0.004); 研究组与研究对照组患者感染发热病例数分别为:23/52例、4/32例(χ2=9.14,P=0.002)。结论 肿瘤化疗病人使用G-CSF外周血白细胞数升高,中性粒细胞NEUT-X参数降低,且感染发热率高于未使用G-CSF患者,推测中性粒细胞颗粒度对评估实体肿瘤化疗病人免疫力具有一定的价值。
Abstract:
Abstract:Objective To study neutral particle particle(NEUT-X)change in the solid tumor patients with chemotherapy by granulocyte colony-stimulating factor(G-CSF).Methods Chose that 52cases of cancer chemotherapy with G-CSF(study group),32 cases of cancer chemotherapy patients without G-CSF(study control group)and 50 cases of healthy(healthy control group).The automatic hematology analyzer Sysmex XE-2100 were been examined the peripheral blood routine and collected the data whichwes the morphological parameters of peripheral white blood cell.The changes ofneutrophil N-X parameters during chemotherapy were analyzed,and the clinicalinfection fever rates of three groups were collected to reveal the relationshipbetween leukocyte morphological parameters and body resistance.Results In the study group,study control group and healthy control group,the NEUT-X was 1 324(890.2,1 358.0),1 440(1 397.3,1 466.3)and 1 329(1 295.1,1 359.4),and the difference was statistically significant between the three groups(F=10.778,P=0.002).In study group,the count ofWBC before and after G-CSF was 0.99(0.22,1.75)×109/L and 7.53(1.00,14.05)×109/L respitively and there was the significant difference(Z=-2.395,P=0.005).In study group patients the NEUT-X was 1 382(1 323.6,1 440.4)and 1 324(890.2,1 358.0)respectively and there was a significant difference(Z=-2.832,P=0.004).Between the study group and the study control group,there were 23/52 casesand 4/32 cases infection in patients with fever case(Z=9.14,P=0.002).Conclusion By G-CSF the leukocyte counts increased inpatients with chemotherapy,and reduced neutrophil NEUT-X parameters,and the infection rate was higher than the non G-CSF patients.The neutrophilgranularity will be useful for evaluating of patients with chemotherapy for solid tumor immunity.

参考文献/References:

[1] 顾玉兰.粒细胞集落刺激因子在化疗患者中的应用调查[J].中国癌症防治杂志,2014,6(2):177-180. Gu YL.Tnvestigation on the application of granulocyte colony stimulating factor in chemotherapy patients[J].Chinese Journal of Oncology Prevention and Treatment,2014,6(2):177-180.
[2] 张 菁,于世英.临床肿瘤学医师对G-CSF使用的认识及化疗和放疗患者使用G-CSF的现状调查[J].肿瘤,2013,33(5):439-445. Zhang J,Yu SY.A survey of clinician's awareness about the use of G-CSF and the current practice of G-CSF use in cancer patients receiving chemotherapy and radiotherapy[J].Tumor,2013,33(5):439-445.
[3] Furundarena JR,Araiz M,Uranga M,et al.The utility of the Sysmex XE-2100 analyzer's NEUT-X and NEUT-Y parameters for detecting neutrophil dysplasia in myelodysplastic syndromes[J].International Journal of Laboratory Hematology,2010,32(3):360-366.
[4] Luo Y,Lin J,Chen H,et al.Utility of neut-X,neut-Y and neut-Z parameters for rapidly assessing sepsis in tumor patients[J].Clinica Chimica Acta,2013,422:5-9.
[5] 胡型忠,徐 克,林 晓,等.中性粒细胞新参数NEUT-X,NEUT-Y在细菌感染中的应用价值研究[J].中华医院感染学杂志,2012,22(5):1095-1098. Hu XZ,Xu K,Lin X,et al.NEUT-X and NEUT-Y are potential diagnostic markers of bacterial infection[J].Chin J Nosocomiol,2012,22(5):1095-1098.
[6] 陈桂芳,邓文军,翟翼方,等.血流感染患者中性粒细胞参数Neut-X和Neut-Y的临床研究[J].国际检验医学杂志,2016,37(23):3283-3284,3287. Chen GF,Deng WJ,Zhai YF,et al.Clinical research of neutrophil parameters NEUT-X and NEUT-Y in patients with blood stream infection[J].Int JLab Med,2016,37(23):3283-3284,3287.
[7] Sachdev R,Goel S,Jha B,et al.Neut-X and Neut-Y:role in multiparametric approach to mds diagnosis[J].International Journal of Laboratory Hematology,2014(36):88.
[8] 陈旭芳.粒细胞集落刺激因子对肿瘤化疗患者白细胞数及医院感染的影响[J].中国肿瘤临床与康复,2009,16(3):196-198. Chen XF.Effects of granulocybe colony stimulating factor on white blood cell count and nosocomial infection in malignant tunor patients after chemotherapy[J].Xhin J Clin Oncol Rehabil,2009,16(3):196-198.
[9] 陈晓陇.粒细胞集落刺激因子(G-CSF)对肿瘤化疗后骨髓细胞数量及形态学改变的影响[D].南宁:广西医科大学,2007. Chen XL.The effect of granulocyte colony-stimulating factor on thechange of bone marrow in cell population and morphology after chemotherapy[D].Nanning:Guangxi Medical University,2007.
[10] Le Roux G,Vlad A,Eclache V,et al.Routine diagnostic procedures of myelodysplastic syndromes:value of a structural blood cell parameter(NEUT-X)determined by the Sysmex XE-2100M[J].International Journal of Laboratory Hematology,2010,326(pt 1):237-243.
[11] Hoffmann C,Hoffmann P,Zimmermann M.Diagno-stic testing for a high-grade inflammation:parameter dynamics and novel markers[J].Clinical Chemistryand Laboratory Medicine,2015,53(4):541-547.
[12] 魏小平,徐 霞,邓穗燕,等.粒细胞集落刺激因子对中性粒细胞碱性磷酸酶及形态学改变的分析[J].热带医学杂志,2009,9(11):1247-1249. Wei XP,XU X,Deng SY,et al.The effects of granulocyte colony stimulating facyor on neutro philic alkaline phosphatase activity and the neutrophil morphlogy[J].Journal of Tropical Medicine,2009,9(11):1247-1249. 收稿日期:2017-11-05 修回日期:2017-11-23 ----------------------------------------------- (上接127页)
[5] Lakhanpal M,Singh LC,Rahman T,et al.Study of single nucleotide polymorphisms of tumour necrosis factors and HSP genes in nasopharyngeal carcinoma inNorth East India[J].Tumor Biol,2016,37(1):271-281.
[6] 王玉珍,吴逸明,李伟辉,等.热休克蛋白70-hom基因+2437(T/C)位点基因多态性与肺癌的关系[J].郑州大学学报(医学版),2010,45(2):219-222. Wang YZ,Wu YM,Li WH,et al.Heat shock protein 70-hom gene+2 437(T/C)polymorphism and susceptibility to lung cancer in Chinese Han population[J].Journal of Zhengzhou University(Medical Scisence),2010,45(2):219-222.
[7] Dutta SK,Girotra M,Singla M,et al.Serum HSP70: a novel biomarker forearly detection of pancreatic cancer[J].Pancreas,2012,41(4):530-534.
[8] Narayanan NK,Narayanan BA,Bosland M,et al.Docosahexaenoic acid in combination with celecoxib modulates HSP70 and p53 proteins in prostate cancer cells[J].Int J Cancer,2006,119(7):1586-1598.
[9] Ferrer-Ferrer M,Malespín-Bendana W,Ramírez V,et al.Polymorphismsin genes coding for HSP-70 are associated with gastric cancer and duodenal ulcer in a population at high risk of gastric cancer in Costa Rica[J].Arch Med Res,2013,44(6):467-474.
[10] Goloudina AR,Demidov ON,Garrido C.Inhibition of HSP70:A challenging anti-cancer strategy[J].Cancer Lett,2012,325(2):117-124.
[11] Sfar S,Saad H,Mosbah F,et al.Synergistic effect and VEGF/HSP70-homhaplotype analysis: relationship to prostate cancer risk and clinical outcome[J].Hum Immunol,2010,71(4):377-382.
[12] Eachkoti R,Qanoon HA,Koul PA,et al.Polymorphic analysis of MHC-linked heat shock protein 70(HSP70-2 AND HSP70-HOM)genes:their susceptibilityand prognostic implications in breast carcinoma cases of Kashmiri Population[J].Asian J Biomed Pharm Sci,2013,3(25):13-20.
[13] Wang YP,Tang Z,Peng BK,et al.Heat shock protein 70 polymorphisms inChinese patients with Graves' disease[J].Genet Mol Res,2015,14(4):18376-18383.
[14] 徐锦雯,赵丽萍,朱春华,等.热休克蛋白70-hom基因多态性与儿童过敏性紫癜的相关性[J].南京医科大学学报(自然科学版),2012,32(8):1075-1078. Xu JW,Zhao LP,Zhu CH,et al.HSP70-hom polymorphism and susceptibilityto childhood Henoch-Schonlein purpura[J].Acta Universitis Medicinals Nanjing(Natural Science),2012,32(8):1075-1078. 收稿日期:2017-08-31 修回日期:2017-09-21

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备注/Memo

备注/Memo:
基金项目:陕西省自然科学基金(2014JM2-8170)。 作者简介:张 磊(1983-),男,内科学硕士,主管检验医师,研究方向:临床血液/体液学检验,E-mail:6168677536@qq.com。
更新日期/Last Update: 2017-12-26