[1]郝文斌,相芬芬,乐红红,等.肝癌细胞单克隆抗体磁珠对人肝癌、胃癌细胞株特异性富集的实验研究[J].现代检验医学杂志,2017,32(06):132-135.[doi:10.3969/j.issn.1671-7414.2017.06.001]
　HAO Wen-bin,XIANG Fen-fen,YUE Hong-hong,et al.Study on the Specificity Enrichment of Hepatoma Cell Monoclonal Antibody Against Hepatocellular Carcinoma and Gastric Cancer[J].Journal of Modern Laboratory Medicine,2017,32(06):132-135.[doi:10.3969/j.issn.1671-7414.2017.06.001]
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肝癌细胞单克隆抗体磁珠对人肝癌、胃癌细胞株特异性富集的实验研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第32卷
期数:
2017年06期
页码:
132-135
栏目:
研究简报·实验技术
出版日期:
2017-12-25

文章信息/Info

Title:
Study on the Specificity Enrichment of Hepatoma Cell Monoclonal Antibody Against Hepatocellular Carcinoma and Gastric Cancer
文章编号:
1671-7414(2017)06-132-04
作者:
郝文斌; 相芬芬; 乐红红; 范 嫣; 许 健; 刘巧丽; 吴 蓉; 康向东
上海中医药大学附属普陀医院检验科,上海 200062
Author(s):
HAO Wen-bin; XIANG Fen-fen; YUE Hong-hong; FAN Yan; XU Jian; LIU Qiao-li; WU Rong; KANG Xiang-dong
Department of Clinical Laboratory,Putuo Hospital Affiliated to Shanghai University of Tradition Chinese Medicine,Shanghai200062,China
关键词:
4-E9;  上皮黏附分子;  循环肿瘤细胞
分类号:
R735; R730.43
DOI:
10.3969/j.issn.1671-7414.2017.06.001
文献标志码:
A
摘要:
目的 探讨肝癌细胞单克隆抗体(4-E9)免疫磁珠与上皮黏附分子(Epcam)磁珠的联合应用能否提高免疫磁珠对人乳腺癌细胞(MCF-7)、人肝癌细胞(BEL-7402)、人胃癌细胞(BGC-823)的富集效率。方法 通过生物素、链霉亲和素将制备的单克隆抗体与磁珠连接,制备成免疫磁珠。通过制备的两种免疫磁珠及两种免疫磁珠的联合应用分别对MCF-7,BEL-7402和BGC-823细胞进行富集率检测。结果 4-E9免疫磁珠的包被率为57.8%,Epcam免疫磁珠的包被率为65.8%。4-E9免疫磁珠对MCF-7,BGC-823和BEL-7402细胞富集率分别为(44±5.33)%,(71±11.33)%和(78.3±8.46)%,Epcam免疫磁珠对BGC-823,BEL-7402和MCF-7细胞富集率分别为(55.5±8.67)%,(78.88±13.11)%和(84.31±6.83)%,两种免疫磁珠联合对BGC-823,BEL-7402和MCF-7细胞富集率分别为(80.4±8.33)%,(85.125±6.77)%和(93.23±4.33)%,联合磁珠组较4-E9免疫磁珠对BGC-823,BEL-7402,MCF-7细胞富集率差异均有统计学意义(P值分别为0.03,0.03,0.04)。联合磁珠组较Epcam免疫磁珠对BGC-823,BEL-7402,MCF-7细胞富集率差异均有统计学意义(P分别为0.04,0.03,0.04)。结论 两种免疫磁珠联合使用能显著提高Epcam免疫磁珠对BEL-7402,BGC-823及MCF-7细胞富集率,4-E9抗体对循环肿瘤细胞的富集可能具有一定的临床应用价值。
Abstract:
Abstract:Objective To investigate whether the combination of 4-E9 immunomagnetic beads and epithelial adhesion molecules(Epacm)beadscan enhance the enrichment efficiency of MCF-7,BEL-7402 and BGC-823 cells.Methods A monoclonal antibody was prepared and ligated with magnetic bead by a biotin and a streptavidin to prepare an immunomagnetic beads.The enrichment rate of MCF-7,BEL-7402 and BGC-823 cells was detected bythe combination of two kinds of immunomagnetic beads and two kinds of immunomagnetic beads.Results The encapsulation rate of 4-E9 immunomagnetic beads was 57.8%,and the encapsulation rate of Epcam immunomagneticbeads was 65.8%.4-E9 immunomagnetic beads on MCF-7,BGC-823 and BEL-7402cell capture rate was(44±5.33)%,(71±11.33)% and(78.3±8.46)% respectively.Epcam immunomagnetic beads on BGC-823,BEL-7402 and MCF-7 cell capture rate was(55.5±8.67)%,(78.88±13.11)% and(84.31±6.83)% respectively.The combination of two kinds of immunomagnetic beads on BGC-823,BEL-7402 and MCF-7cell capture rate was(80.4±8.33)%,(85.125±6.77)% and(93.23±4.33)% respectively.Joint beads group compared with 4-E9 immunomagnetic beads on BGC-823,BEL-7402 and MCF-7 cell enrichment rate were statistically significant(P=0.03,0.03,0.04),and joint beads group compared with 4-E9 immunomagnetic beads on BGC-823,BEL-7402 and MCF-7 cell enrichment rate were statistically signific(P=0.04,0.03,0.04).ConclusionThe combination of two kinds of immunomagnetic beads can significantly improve the enrichment efficiency of Epcam immunomagnetic beads on BEL-7402,BGC-823 and MCF-7 cells.4-E9 antibody enrichment of circulating tumor cells may have some clinical value.

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备注/Memo

备注/Memo:
基金项目:上海市普陀区卫计委/普陀区科委(2015PTKW013)。 作者简介:郝文斌(1990-),男,本科,在读研究生,E-mail:15002120779@163.com。 通讯作者:康向东,E-mail:xd_kang@163.com。
更新日期/Last Update: 2017-12-26