[1]任 强,付钰淋,李 华,等.陕西省新发现艾滋病感染者HIV耐药警戒线调查研究[J].现代检验医学杂志,2017,32(06):136-138.[doi:10.3969/j.issn.1671-7414.2017.06.001]
　REN Qiang,FU Yu-lin,LI Hua,et al.Investigation on Drug Resistance Threshold of Newly Found HIV Infected Persons in Shaanxi[J].Journal of Modern Laboratory Medicine,2017,32(06):136-138.[doi:10.3969/j.issn.1671-7414.2017.06.001]
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陕西省新发现艾滋病感染者HIV耐药警戒线调查研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第32卷
期数:
2017年06期
页码:
136-138
栏目:
研究简报·实验技术
出版日期:
2017-12-25

文章信息/Info

Title:
Investigation on Drug Resistance Threshold of Newly Found HIV Infected Persons in Shaanxi
文章编号:
1671-7414(2017)06-136-03
作者:
任 强; 付钰淋; 李 华; 邹扬帆; 张梦妍; 张 超; 常文辉
陕西省疾病预防控制中心,西安 710054
Author(s):
REN Qiang; FU Yu-lin; LI Hua; ZOU Yang-fan; ZHANG Meng-yan; ZHANG Chao; CHANG Wen-hui
Shaanxi Provincial Center for Disease Control and Prevention,Xi'an 710054,China
关键词:
艾滋病病毒;  耐药基因;  耐药警戒线;  亚型
分类号:
R512.91; Q786
DOI:
10.3969/j.issn.1671-7414.2017.06.001
文献标志码:
A
摘要:
目的 分析新发现且尚未治疗的艾滋病病毒(HIV)感染人群的耐药基因序列,了解陕西省HIV耐药毒株的传播情况和流行特点。方法 根据世界卫生组织推荐的艾滋病病毒耐药警戒线调查方法(HIVDR-TS),对47例16～25岁新确证HIV感染者的阳性血清,用In-house方法测定pol基因区部分序列,采用在线CPR软件判断是否存在传播性耐药基因突变,并提交至美国斯坦福大学HIV在线耐药数据库中进行耐药程度分析,使用MEGA5.0软件构建进化树,分析亚型。结果 47份样本中没有发现传播性耐药基因突变,但有14份样本发现有与耐药相关的基因突变位点,包括蛋白酶区的T69N,E138A和V179D/E突变位点,反转录酶区的A71T/V和L10I/V突变位点; 其中在CRF01_B亚型的一份样本中出现了K103R位点,其与V179E存在协同作用,导致对依非韦伦(EFV)和奈韦拉平(NVP)等药物的中度耐药。其余13份样本中发现的突变位点或可引起部分药物的低度潜在耐药,没有实际临床意义。基于pol区基因型分型,共有四种亚型,其中以CRF01_AE为主(26/47),其次为CRF07_BC(17/47)。结论 陕西省HIV耐药株流行率<5%,属于低度流行水平,个体治疗前尚不需要进行耐药性检测,同时还需定期进行耐药警戒线调查研究。
Abstract:
Abstract:Objective To analyze the resistance gene sequences of newly untreated HIV infected people,and to understand the prevalence and epidemic characteristics of HIV drug-resistant strains in Shaanxi Province.Methods According to the recommended by the World HealthOrganization HIV drug resisitance threshold(HIVDR-TS),sera from 47 newly diagnosed HIV infected patients aged 16～25 years were analyzed.In-house determination of pol gene partial sequence,using online CPR software to determine whether there was transmission of resistant mutants,and submitted to Stanford University in the United States online HIV drug resistance database for the extent ofthe drug resistance analysis,the phylogenetic tree was constructed using mega 5.0 software,analysis of subtypes.Results No spread drug mutations were found in 47 samples,but there were 14 samples that were foundto have associated with resistance gene mutation sites,including the RT regionT69N and E138A,V179D/E mutations,PR A71T/V,L10I/V mutation sites and in subtype CRF01_B a like this in the site K103R,with V179E and there was a synergistic effect,resulting in accordance with efavirenz(EFV)and nevirapine(NVP)drugs such as moderately resistant.The mutations found in the remaining 13 sampleswould lead to a low degree of resistance to some of the drugs,and there was noclinical significance.Conclusion The prevalence rate ofHIV resistant strains in Shaanxi province was <5%,which be longed to the low epidemic level.It is not necessary to detect drug resistance before individual treatment,and it is necessary to carry out the investigation of drug resistance.

参考文献/References:

[1] Havlir D,Vella S,Hammer S.The global HIV drug resistance surveillance program:a partnership between WHO and IAS.International AIDS Society[J].AIDS,2002,16(10):7-9.
[2] 童 毅,邢 辉,申莉梅,等.贵州省2015年HIV耐药警戒线调查[J].临床医学研究与实践,2016,1(15):100. Tong Y,Xing H,Shen LM,et al.Surveillance of HIV drug resistancein Guizhou Province in 2015[J].Clinical Research and Practice,2016,1(15):100.
[3] 孙晓光,于海英,苏生利,等.山东省2013～2015年新发现未治疗HIV-1感染者耐药毒株传播警戒线调查[J].中华预防医学杂志,2017,51(7):604-609. Sun XG,Yu HY,Su SL,et al.Survey of HIV-1 drug resistance threshold in shandong province in 2013-2015[J].Chinese Journal of Preventive Medicine,2017,51(7):604-609.
[4] World Health Organization.Guidelines for surveillance of HIV drug resistance[R].Switzerland:Geneva,WHO,2003:30-36.
[5] Bennett DE,Camacho RJ,Otelea D,et al.Drug resistance mutations for surveillance of transmitted HIV-1 drug-resistance:2009 Update[J].PLoS One,2009,4(3):e4724.
[6] Levy JA.HIV and the pathogenesis of AIDS[M].//Shao YM.Beijing:Science Press,2000:289-290.
[7] Shafer RW.Genotypic testing for human immunodeficiency virus type 1 drugresistance[J].Clin Microbiol Rev,2002,15(2):247-277.
[8] Beinker NK,Mayers DL,Lange CG,et al.Genotypic drug resistance and cause of death in HIV-infected persons who died in 1999[J].J Acquir Immune Defic Syndr,200l,28(3):250-253.
[9] 张 娜,蒋 岩,廖清华,等.2013年江西省HIV-1耐药警戒线调查[J].中国卫生检验杂志,2015,25(3):425-428. Zhang N,Jiang Y,Liao QH,et al.Drug resisitance threshold surveyof HIV-1 in Jiangxi province in 2013[J].Chinese Journal of Health LaboratoryTechnology,2015,25(3):425-428.
[10] 周全华,凌 华,邢 辉,等.重庆市HIV-1耐药警戒线调查[J].现代预防医学,2013,40(20):3860-3862,3864. Zhou QH,Ling H,Xing H,et al.Survey on the drug resisitance threshold of HIV-1 in Chongqing[J].Modern Preventive Medicine,2013,40(20):3860-3862,3864.
[11] 徐晓琴,郭宏雄,胡海洋,等.江苏省连续三年HIV-1耐药警戒线及流行亚型的调查研究[J].南京医科大学学报(自然科学版),2015,35(9):1329-1332. Xu XQ,Guo HX,Hu HY,et al.Investigation on HIV-1 drug resistant alertline and epidemic subtype in Jiangsu province for three years[J].Acta Universitis Medicinalis Nanjing(Natural Sciences),2015,35(9):1329-1332.
[12] 任 强,常文辉,张梦妍,等.2009～2014年陕西省艾滋病哨点监测重点人群HIV感染和新发感染检测分析[J].现代检验医学杂志,2015,30(3):56-59. Ren Q,Chang WH,Zhang MY,et al.Analysis of HIV infection and new infections detection of AIDS sentinel surveillance focus groups in Shaanxi Province 2009～2014[J].Journal of Modern Laboratory Medicine,2015,30(3):56-59. 收稿日期:2017-09-28 修回日期:2017-10-23

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备注/Memo

备注/Memo:
基金项目:陕西省卫生与计划生育委员会科研基金项目:2012E2。 作者简介:任 强(1983-),男,西安交通大学硕士研究生,主管检验师,研究方向:艾滋病防治与实验室检测。E-mail:175538852@qq.com。 通讯作者:常文辉(1972-),女,博士研究生,副主任医师,艾滋病综合防治,E-mail:429674121@qq.com。
更新日期/Last Update: 2017-12-26