[1]虞红燕,刘 禹,郁大伟,等.2015～2017年上海地区人群血浆PC和PS活性检测情况的调查分析[J].现代检验医学杂志,2019,34(04):154-157.[doi:10.3969/j.issn.1671-7414.2019.04.038]
　YU Hong-yan,LIU Yu,YU Da-wei,et al.Investigation and Analysis of Plasma PC and PS Activities in Shanghai Population from 2015 to 2017[J].Journal of Modern Laboratory Medicine,2019,34(04):154-157.[doi:10.3969/j.issn.1671-7414.2019.04.038]
点击复制

2015～2017年上海地区人群血浆PC和PS活性检测情况的调查分析()
分享到：

《现代检验医学杂志》[ISSN:/CN:]

卷:
第34卷
期数:
2019年04期
页码:
154-157
栏目:
质量控制·实验室管理
出版日期:
2019-08-20

文章信息/Info

Title:
Investigation and Analysis of Plasma PC and PS Activities in Shanghai Population from 2015 to 2017
文章编号:
1671-7414(2019)04-154-04
作者:
虞红燕1; 刘 禹2; 郁大伟3; 梁 茜2; 王梦汝3; 许冠群2; 丁 磊3
(1.解放军第904医院 苏州院区检验科,江苏苏州 215000; 2.上海交通大学医学院附属瑞金医院检验科,上海 200001; 3.苏州市第五人民医院 苏州大学附属传染病医院检验科,江苏苏州 215000)
Author(s):
YU Hong-yan1; LIU Yu2; YU Da-wei3; LING Qian2; WANG Meng-ru3; XU Guan-qun2; DING Lei3
(1.Department of Clinical Laboratory,PLA 904 Hospital Suzhou District,Jiangsu Suzhou 215000, China; 2.Department of Clinical Laboratory,Ruijin Hospital,Shanghai Jiaotong University School of Medicine,Shanghai 200001,China; 3.Department of Clinical Laboratory,the Fifth People's Hospital of Suzhou,Infectious Disease Hospital,Suzhou University,Jiangsu Suzhou 215000,China)
关键词:
蛋白C;  蛋白S;  易栓症;  异常率
分类号:
R446.112
DOI:
10.3969/j.issn.1671-7414.2019.04.038
文献标志码:
A
摘要:
目的 分析目前上海市及周边地区医院实验室对血浆蛋白C活性(PC:A)和蛋白S活性(PS:A)的检测情况,以及临床上对其临床意义的认知情况。方法 回顾性分析2015年1月～2017年12月上海市瑞金医院检验科出凝血实验室对PC:A和PS:A检测情况,收集外单位送检标本(n=20)进行4℃环境保存下不同时间检测PC:A和PS:A,通过EXCEL,Graphpad Prism5.0统计软件进行数据分析。结果 门诊、住院、外单位送检标本及其检测异常率均呈现增长趋势,其中门诊标本量最大,外单位送检量次之,住院标本量最少,2015～2017年度总体异常率分别为4.6%,5.8%和7.8%; 外单位送检共12家医院性质分为:公立医院4家,第三方实验室3家,外资医院2家,民营医院3家,其中外资医院中PC:A和PS:A检测异常率最高,第三方实验室次之; 在4℃环境下不同时间检测结果与即刻相比,PC:A和PS:A在第1,2,3天结果整体呈现平稳下降趋势,差异无统计学意义(P>0.05),PS:A检测结果第3天呈现异常波动,可能与其运输过程中标本保存不当引起污染、变质有关。结论 部分临床医生对血浆蛋白PC和PS的临床意义认知肤浅或缺乏,上海及周边地区医院开展血浆PC和PS活性检测的实验室较少,抗凝蛋白活性检测技术及临床应用必须加强宣传教育和推广普及。
Abstract:
Objective To analyze the detection of plasma protein C activity(PC:A)and protein S activity(PS:A)in hospitals of Shanghaiand surrounding areas,as well as the clinical understanding of its clinical significance.Methods A retrospective investigation was conducted on the detection of plasma PC and PS specimens from the coagulation laboratory of Shanghai Ruijin Hospital from January 2015 to December 2017,and collected other units samples(n=20)and preserved at 4℃ environment,then teststhe plasma activity of PC,PS under different time,and data were analyzed by EXCEL and Graphpad Prism 5.0 statistical software.Results Outpatient,inpatient,and external unit inspection specimens and their abnormal rate of detection showed an increasing trend,among them,the amount of outpatient specimens was the largest.The number of specimens sent by external unitswas second,and the number of hospitalized specimens was the least.The overallabnormal rate were 4.6%,5.8% and 7.8% respectively in 2015～2017.According to the nature of the hospital,there were 12 out-of-bound units,which were divided into four public hospitals,three third-party laboratories,two foreign-funded hospitals and three private hospitals.Among them,the abnormal ratesof PC:A and PS:A in foreign-funded units were the highest,followed by the third-party laboratories.Compared with the immediate test results in 4° environment,PC:A and PS:A showed a steady downward trend on the 1,2 and 3 days,and the difference was not statistically significant(P>0.05).PS:A test results showed abnormal fluctuations on the 3 day,which would be related to the pollution and deterioration caused by improper preservation of specimens during transportation.Conclusion Some clinicians had superficialor insufficient understanding of the clinical significance of plasma protein PCand PS.There were fewer laboratories in hospitals in Shanghai and its surrounding areas to carry out the detection of plasma PC and PS activity.It is necessary to strengthen the popularization and education of anticoagulant protein activity detection technology and its clinical application.

参考文献/References:

[1] EGEBERG O.Inherited antithrombin deficiency cau-sing thrombophilia[J].Thromb Diath Haemorrh,1965,13:516-530. [2] 朱铁楠,赵永强.2012版易栓症诊断中国专家共识解读[J].临床血液学杂志,2013,26(3):156-157. ZHU Tienan,ZHAO Yongqiang.Interpretation of the Chinese expert consensus on the diagnosis of thrombosis in 2012[J].Journal of Clinical Hematology(China),2013,26(3):156-157. [3] OKAJIMA K.Prevention of endothelial cell injury by activated protein C:the molecular mechanism(s)and therapeutic implications[J].Curr Vasc Pharmacol,2004,2(2):125-133. [4] 赵慧茹,王聪,苏玉,等.关节置换围术期患者血浆内皮细胞蛋白C受体和血栓调节蛋白水平变化分析[J].现代检验医学杂志,2018,33(2):125-126,133. ZHAO Huiru,WANG Cong,SU Yu,et al.Analysis of changes in plasma endothelial protein C receptor and thrombomodulin levels in patients withjoint replacement during perioperative period[J].Journal of Modern Laboratory Medicine,2018,33(2):125-126,133. [5] 刘秀丽,周文宾,李臣宾,等.抗凝蛋白检测现状全国性调查与分析[J].中华医学杂志,2017,97(22):1699-1704. LIU Xiuli,ZHOU Wenbin,LI Chenbin,et al.Study and analysis of national current status and problems of anticagulant proteins assay[J].Natl MedJ China,2017,97(22):1699-1704. [6] ZHU Tienan,DING Qiulan,BAI Xia,et al.Normal ranges and genetic variants of antithrombin,protein C and protein S in the general Chinese population.Results of the Chinese Hemostasis Investigation on Natural Anticoagulants Study IGroup[J].Haematologica,2011,96(7):1033-1040. [7] 陈慧芬.产科易栓症的检测在反复自然流产病因诊断中的临床价值[J].中华检验医学杂志,2017,40(11):856-859. CHEN Huifen.The clinical value of the detection of obstetrical thrombophilia in the diagnosis of recurrent spontaneous abortion[J].Chinese Journal of Laboratory Medicine,2017,40(11):856-859. [8] 李蕾,王培昌.蛋白C抗凝系统及生物学作用最新研究进展[J].四川医学,2012,33(9):1685-1687. LI Lei,WANG Peichang.The latest research progress of protein C anticoagulant system and its biological effects[J].Sichuan Medical Journal,2012,33(9):1685-1687. [9] 孟景晔,彭武建,骆子义,等.全外显子测序检测一个遗传性易栓症家系的致病基因[J].中国优生与遗传杂志,2017,25(4):42-44. MENG Jingye,PENG Wujian,LUO Ziyi,et al.Identification of causative gene in a Chinese family with inherited thrombophilia by whole exome sequencing[J].Chinese Journal of Birth Health & Heredity,2017,25(4):42-44. [10] MOSNIER L O,ZLOKOVIC B V,GRIFFIN J H.Cytoprotective-selective activated protein C therapy for ischaemic stroke[J].Thromb Haemost,2014,112(5):883-892. [11] CHRISTIAANS S C,WAGENER B M,ESMON C T,et al.Protein C and acute inflammation:a clinical and biological perspective[J].Am J Physiol Lung Cell MolPhysiol,2013,305(7):L455-L466. [12] 谢燕,唐巧云,郑海建,等.2型糖尿病肾病凝血异常相关因素分析[J].中国综合临床,2012,28(10):1009-1012. XIE Yan,TANG Qiaoyun,ZHENG Haijian,et al.Von willebrand factor research on related factor of coagulation abnormalities in type 2 diabetic nephropathy[J].Clinical Medicine of China,2012,28(10):1009-1012. [13] ZHU Tienan,ZHAO Yongqiang,DING Qiulan,et al.The activity levels andprevalence of deficiency of protein C,protein S and antithrombin in Chinese Hanpopulation[J].Chinese Journal of Hematology,2012,33(2):127-130.

备注/Memo

备注/Memo:
基金项目:国家自然科学基金(81400100)。 作者简介:虞红燕(1988-),女,本科,主管检验师,研究方向:出血及血栓实验室检测工作,E-mail:578457931@qq.com。 通讯作者:丁 磊(1988-),男,本科,主管检验师,研究方向:出血及血栓实验室检测工作,E-mail:dinglei_0126@126.com。 许冠群,E-mail:rj_40442@163.com,共同通讯作者。 收稿日期:2019-01-15 修回日期:2019-05-16
更新日期/Last Update: 2019-07-30