[1]许鹏宇,李冬秀,郭瑞娟,等.不同浓度乙醇对人精子形态、前向运动力和DNA 碎片指数影响的体外实验研究[J].现代检验医学杂志,2020,35(03):97-99.[doi:10.3969/j.issn.1671-7414.2020.03.025]
 XU Peng-yu,LI Dong-xiu,GUO Rui-juan,et al.Study on the Effects of Different Concentration Alcohol on Motility, Morphology and DNA Fragment Index of Spermatozoa Cultured in Vitro[J].Journal of Modern Laboratory Medicine,2020,35(03):97-99.[doi:10.3969/j.issn.1671-7414.2020.03.025]
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不同浓度乙醇对人精子形态、前向运动力和DNA 碎片指数影响的体外实验研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第35卷
期数:
2020年03期
页码:
97-99
栏目:
研究简报·实验技术
出版日期:
2020-07-20

文章信息/Info

Title:
Study on the Effects of Different Concentration Alcohol on Motility, Morphology and DNA Fragment Index of Spermatozoa Cultured in Vitro
文章编号:
1671-7414(2020)03-097-04
作者:
许鹏宇 李冬秀郭瑞娟程立立
(河北医科大学第三医院生殖医学科,石家庄 050051)
Author(s):
XU Peng-yu LI Dong-xiu GUO Rui-juan CHENG Li-li
(Reproductive Medicine Center, the Third Hospital of Hebei Medical University, Shijiazhuang 050051, China)
关键词:
乙醇正常形态精子前向运动精子DNA 碎片指数体外培养
分类号:
R446.19
DOI:
10.3969/j.issn.1671-7414.2020.03.025
摘要:
目的 探索不同浓度乙醇对体外培养的人精子形态、前向运动力和DNA 碎片指数的影响。方法 采用密度梯 度离心法处理精液后将回收的30 份精子混悬于不同乙醇浓度的精子培养液中,其中对照组0 mg/dl(A 组),实验组(低 浓度)20 mg/dl(B 组),C 组(中浓度)80 mg/dl 和D 组(高浓度)160 mg/dl。6h 后检测各组正常形态精子比例、前 向运动精子以及精子DNA 碎片指数。结果 B 组正常形态精子(9.60±2.13)% 与A 组(10.23±2.48)% 相比,差异 无统计学意义(P=0.128)。C 组(8.03±1.96)% 和D 组(5.23±1.23)% 正常形态精子低于A 组,差异有统计学意义 (P=0.011,0.000)。前向运动精子比例依次下降[A 组(73.03±2.83)%,B 组(63.13±4.22)%,C 组(53.03±3.05)%, D组(45.07±2.97)%],差异有统计学意义(F=283.454,P=0.000)。D组精子DFI (20.30±5.06)%,高于A组(12.37±3.68)%、 B组(12.67±3.78)% 和C 组(15.70±4.06)%,差异均有统计学意义( P=0.000,0.000,0.001),B 组、C 组精子 DFI 高于A 组,但差异均无统计学意义(P=0.804,0.339),B 组与C 组精子DFI 比较,差异亦无统计学意义(P=0.478)。
Abstract:
Objective To explore the effects of different concentration alcohol on motility, morphology and DNA fragment index of spermatozoa cultured in vitro. Methods Thirty spermatozoa samples were prepared by density gradient centrifugation and the recovered spermatozoa was suspended in the culture medium with different concentration of alcohol. The control group with concentration of alcohol 0 mg/dl was named as group A , the experimental groups with concentration of alcohol 20mg/ dl , 80mg/dl and 160mg/dl were named as group B (low concentration), group C (medium concentration) and group D (high concentration). 6 hours later, the ratio of progressive motility, normal morphology and spermatozoa DNA fragmention index (DFI) were detected in each group. Results There was no significant difference (P=0.128) between group B (9.60±2.13) % and A(10.23±2.48) % about the ratio of normal morphology spermatozoa , but the ratio of normal morphology spermatozoa in group C(8.03±1.96)% and group D(5.23±1.23) % were significantly lower than that in group A (P=0.011,0.000). The ratio of progressive motility [group A(73.03±2.83)%, group B(63.13±4.22)%, group C(53.03±3.05)%, group D(45.07±2.970%] decreased and the difference was statistically significant(F=283.454, P<0.05). Spermatozoa DNA fragmentation index(DFI) of group D(20.30±5.06) % was higher than that of group A(12.37±3.68) %, group B(12.67±3.78) % and group C(15.70±4.06) %, and the difference was statistically significant(P=0.000, 0.000,0.001). DFI in group B and group C was higher than that in group A, but the difference was not statistically significant (P=0.804,0.339). Also, there was no significant difference between groups B and group C (P=0.478). Conclusion Alcohol can reduce the ratio of progressive motility and normal morphology sperm and increase sperm DFI.

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备注/Memo

备注/Memo:
收稿日期:2019-12-13 修回日期:2020-02-09基金项目:河北省卫健委项目(No:20190623)。 作者简介:许鹏宇(1985-),男,硕士,主治医师,主要研究方向:辅助生殖技术及男性不育的诊治,E-mail:xupengyushen@163.com。 通讯作者:李冬秀,女,博士,副主任医师,主要研究方向:辅助生殖实验室技术及女性不孕症的诊治,E-mail:lidongxiu1976@163.com
更新日期/Last Update: 2020-07-20