[1]胡　伟,张　涛,盛尚春,等.IGF-1R通过调控JAK/STAT信号通路对脓毒症大鼠作用机制的研究[J].现代检验医学杂志,2022,37(02):6-11.[doi:10.3969/j.issn.1671-7414.2022.02.002]
　HU Wei,ZHANG Tao,SHENG Shang-chun,et al.Mechanism of IGF-1R on Sepsis Rats by Regulating JAK / STAT Signaling Pathway[J].Journal of Modern Laboratory Medicine,2022,37(02):6-11.[doi:10.3969/j.issn.1671-7414.2022.02.002]
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IGF-1R通过调控JAK/STAT信号通路对脓毒症大鼠作用机制的研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第37卷
期数:
2022年02期
页码:
6-11
栏目:
论　著
出版日期:
2022-03-15

文章信息/Info

Title:
Mechanism of IGF-1R on Sepsis Rats by Regulating JAK / STAT Signaling Pathway
文章编号:
1671-7414（2022）02-006-06
作者:
胡　伟; 张　涛; 盛尚春; 刘　翔; 丁贵梅; 代　琼
（宜宾市第二人民医院检验科，四川宜宾 644000）
Author(s):
HU Wei;  ZHANG Tao; SHENG Shang-chun;  LIU Xiang;  DING Gui-mei;  DAI Qiong
（Department of Clinical Laboratory,the Second People’s Hospital of Yibin, Sichuan Yibin 644000，China）
关键词:
1型胰岛素样生长因子受体; Janus激酶/信号转导和转录激活因子信号通路; 脓毒症
分类号:
R-332
DOI:
10.3969/j.issn.1671-7414.2022.02.002
文献标志码:
A
摘要:
目的　探究1 型胰岛素样生长因子受体（insulin-like growth factor 1 receptor，IGF-1R）通过调控Janus 激酶/ 信号转导和转录激活因子（janus kinase/signal transducer and activvator of transcription，JAK/STAT）信号通路对脓毒症大鼠作用机制。方法　将60 只实验大鼠随机分为假手术组（sham 组）、脓毒症大鼠模型组（sepsis 组）和脓毒症大鼠模型给予IGF-1R 干预组（IGF-1R 组），每组20 只。检测肺组织中IGF-1R 阳性细胞比例（免疫荧光染色法），检测血清中IL-6 和TNF-α 含量（ELISA 法），检测肺组织病理学改变（HE 染色法），TUNEL 检测各组细胞凋亡率，免疫印迹法检测组织中p-JAK1 和p-STAT3 蛋白表达。结果　组织中IGF-1R 阳性细胞在sham 组（58.32%±5.21%），sepsis 组（21.65%±8.73%）和IGF-1R 组（46.58%±6.72%）占比比较差异有统计学意义（F=141.7，P<0.000 1）；和sham 组相比，sepsis 组大鼠肺组织中IGF-1R 阳性细胞在总细胞中所占比例减少（t=16.13，P<0.000 1），IGF-1R 组大鼠肺组织中IGF-1R 阳性细胞在总细胞中所占比例高于sepsis 组（t=10.12，P<0.000 1），差异均有统计学意义。血清中IL-6 在sham组（4.35±2.01pg/ml）、sepsis 组（9.81±4.26pg/ml）和IGF-1R 组（5.11±2.57pg/ml）中的含量比较差异有统计学意义（F=18.23，P<0.000 1），血清中TNF-α 含量在sham 组（2.31±1.12pg/ml）、sepsis 组（14.15±3.26pg/ml）和IGF-1R 组（6.58±2.15pg/ml）比较差异有统计学意义（F=130.7，P<0.000 1），和sham 组相比，sepsis 组IL-6 和TNF-α含量升高（t=5.18，21.98，均P<0.000 1）；IGF-1R 组IL-6 和TNF-α 含量较sepsis 组降低，差异具有统计学意义（t=4.23，12.01, 均P< 0.000 1）。细胞凋亡率在sham 组（6.35%±2.51%）、sepsis 组（37.84%±6.62%）和IGF-1R 组（22.71%±4.28%）间比较差异有统计学意义（F=217.4，P<0.000 1）。和sham 组相比，sepsis 组细胞凋亡率增多（t=19.89，P<0.000 1）；和sepsisi 组相比，IGF-1R 组大鼠肺组织中凋亡率降低（t=8.583，P<0.000 1）。肺组织中p-JAK1 在sham 组（0.15±0.11）、sepsis 组（0.33±0.16）和IGF-1R 组（0.17±0.13）中表达差异有统计学意义（F=10.70，P=0.000 1）。肺组织中p-STAT3在sham 组（0.12±0.10）、sepsis 组（0.28±0.15）和IGF-1R 组（0.19±0.12）中表达差异有统计学意义（F=8.230，P=0.000 7）。和sham 组相比，sepsis 组p-JAK1 和p-STAT3 蛋白表达增加（t=4.146，3.969，P=0.000 2, 0.000 3）；IGF-1R 组大鼠肺组织中p-JAK1 和p-STAT3 蛋白表达低于sepsis 组（t=3.471，2.095，P=0.001 3, 0.042 9）。结论　IGF-1R 可通过调控JAK/STAT 信号通路改善脓毒症所致大鼠的肺损伤，降低血清中炎症因子的含量。
Abstract:
Objective To explore the mechanism of insulin-like growth factor 1 receptor (IGF-1R) on sepsis rats by regulating, janus kinase/signal transducer and activvator of transcription (JAK/STAT) signa pathway. Methods　Sixty experimental rats were randomly divided into sham operation group(sham group), sepsis rat model group(sepsis group) and sepsis rat model group given IGF-1R intervention group(IGF-1R group), with 20 rats in each group. The proportion of IGF-1R positive cells in rat lung tissue was detected by immunofluorescence staining. The contents of IL-6 and TNF-α in serum were detected by ELISA. The pathological changes of lung tissue were detected by HE staining. Cell apoptosis in each group was detected by TUNEL. The protein expression of p-JAK1 and p-STAT3 in tissues was detected by Western blotting. Results　The proportion of IGF-1R positive cells in the sham group (58.32%±5.21%), sepsis group (21.65%±8.73%) and IGF-1R group (46.58%±6.72%) was statistically significant (F=141.7, P<0.000 1).Compared with the sham group, the number of IGF-1R positive cells in the sepsis group was decreased (t=16.13, P<0.000 1), and the number of IGF-1R positive cells in the sepsis group was higher than that in the sepsis group (t=10.12, P<0.000 1), the differences were statistically significant, respectively. The serum IL-6 levels in the sham group (4.35±2.01pg/ml), the sepsis group (9.81±4.26pg/ml) and the IGF-1R group (5.11±2.57pg/ml) were statistically significant different (F=18.23, P<0.000 1). The serum TNF-α levels in the sham group (2.31±1.12pg/ml), sepsis group (14.15±3.26pg/ml) and IGF-1R group (6.58±2.15pg/ml) were significantly different (F=130.7, P<0.000 1). Compared with the sham group, IL-6 and TNF-α increased in the sepsis group (t=5.18, 21.98, all P<0.000 1). The levels of IL-6 and TNF-α in IGF-1R group were lower than those in sepsis group (t=4.23, 12.01, all P<0.000 1). The cell apoptosis rate was significantly different between the sham group (6.35%±2.51%), the sepsis group (37.84±6.62%) and the IGF-1R group (22.71%±4.28%) (F=217.4, P<0.000 1). Compared with the sham group, the apoptosis rate of the sepsis group was increased (t=19.89, P<0.000 1). Compared with the sepsisi group, the percentage of apoptotic cells in the IGF-1R group was lower (t=8.583, P<0.000 1), the differences were statistically significant, respectively. The expression of P-JAK1 in lung tissue showed statistically significant difference in sham group (0.15±0.11), sepsis group (0.33±0.16) and IGF-1R group (0.17±0.13) (F=10.70, P=0.000 1). The expression of p-STAT3 in lung tissue was significantly different in sham group (0.12±0.10), sepsis group (0.28±0.15) and IGF- 1R group (0.19±0.12) (F=8.230, P=0.000 7). Compared with sham group, the expression of p-JAK1 and p-STAT3 proteins increased in sepsis group (t =4.146, 3.969, P =0.000 2, 0.000 3). The protein expressions of p-JAK1 and p-STAT3 in the lung tissues of rats in the IGF-1R group were lower than those in the sepsis group (t =3.471, 2.095, P =0.001 3, 0.042 9). Conclusion　IGF-1R can improve the lung injury caused by sepsis by regulating the JAK/STAT signaling pathway and reduce the content of inflammatory factors in serum of rats.

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备注/Memo

备注/Memo:
基金项目：国家自然科学基金委员会资助项目（B1702101）。
作者简介：胡伟（1976-），男，大学本科，副主任技师，研究方向：临床检验医学，E-mail:luckycc733@sina.com。
通讯作者：代琼（1966-），女，大学本科，主任技师，研究方向：临床输血，E-mail:371119334@qq.com。
更新日期/Last Update: 1900-01-01