[1]张明威a,陈　阳a,王　静a,等.肾细胞癌患者癌组织和血清微囊泡miR-378表达水平的临床应用价值研究[J].现代检验医学杂志,2022,37(02):1-5.[doi:10.3969/j.issn.1671-7414.2022.02.001]
　ZHANG Ming-weia,CHEN Yanga,WANG Jinga,et al.Clinical Value of miR-378 Expression Levels in Tumor Tissue and Serum Extracellular Vesicles for Renal Cell Carcinoma Patients and Its Function Prediction[J].Journal of Modern Laboratory Medicine,2022,37(02):1-5.[doi:10.3969/j.issn.1671-7414.2022.02.001]
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肾细胞癌患者癌组织和血清微囊泡miR-378表达水平的临床应用价值研究()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第37卷
期数:
2022年02期
页码:
1-5
栏目:
论　著
出版日期:
2022-03-15

文章信息/Info

Title:
Clinical Value of miR-378 Expression Levels in Tumor Tissue and Serum Extracellular Vesicles for Renal Cell Carcinoma Patients and Its Function Prediction
文章编号:
1671-7414（2022）02-001-06
作者:
张明威a; 陈　阳a; 王　静a; 葛京平b; 张春妮a; 汪俊军a; 王　成a
（中国人民解放军东部战区总医院a．检验科；b．泌尿外科，南京210002）
Author(s):
ZHANG Ming-weia;  CHEN Yanga;  WANG Jinga;  GE Jing-pingb;  ZHANG Chun-nia;  WANG Jun-juna;  WANG Chenga
(a.Department of Clinical Laboratory；b. Department of Urology,General Hospital of Eastern Theater Commend,PLA, Nanjing 210002, China)
关键词:
肾细胞癌; 微小核糖核酸-378; 微囊泡
分类号:
R737.11；R730.43
DOI:
10.3969/j.issn.1671-7414.2022.02.001
文献标志码:
A
摘要:
目的　检测肾细胞癌（renal cell carcinoma, RCC）患者癌组织和血清微囊泡（extracellular vesicles, EVs）中微小核糖核酸（micro ribonucleic acid，miR）-378 表达变化，评估其对于RCC 辅助诊断价值。方法　收集2014 年1 月～ 2018年12 月东部战区总医院泌尿外科初收的RCC 患者癌组织和癌旁组织40 对；同时，收集RCC 患者及年龄、性别匹配的健康对照血清标本各60 例。运用实时荧光定量聚合酶链反应技术（real-time quantitative polymerase chain reaction, qRTPCR）检测组织、血清EVs 中miR-378 表达水平变化，评估RCC 癌组织和患者血清EVs 中miR-378 表达水平测定对于患者辅助诊断价值，进一步通过生物信息学分析miR-378 参与RCC 的生理病理功能。结果　qRT-PCR 结果显示，与癌旁正常组织[0.134 (0.054，0.546)] 相比，miR-378 在RCC 组织中[0.050 (0.016，0.137)] 表达显著降低，差异有统计学意义（Z = -3.481， P = 0.003）；同时，RCC 患者血清EVs 中miR-378 水平[8.295 (4.930， 15.60)] 与健康对照[3.501(2.005， 6.853)] 相比显著升高，差异具有统计学意义（U = 850，P ＜ 0.001）。受试者工作特征曲线（receiver operating characteristic，ROC）分析结果显示，组织中miR-378 相对含量对于RCC 患者诊断的ROC 曲线下面积（AUC）为0.665（95% CI：0.544 ～ 0.786）；血清EVs 中miR-378 水平对于RCC 诊断的AUC 为0.764（95% CI：0.680 ～ 0.848）。生物信息学分析miR-378 功能结果显示：miR-378 主要涉及的生物过程有分解代谢、细胞骨架蛋白调节和蛋白激酶活力调节。结论　RCC 癌组织和血清EVs 中miR-378 表达测定对于RCC 患者具有一定的辅助诊断价值，其中血清EVs miR-378 测定更具优势；miR-378 可通过调控多种生物学过程及功能参与RCC 发生发展。
Abstract:
Objective　To evaluate the clinical value of miR-378 measurement in tumor tissue and serum extracellular vesicles (EVs) for renal cell carcinoma (RCC) patients, and further predict the biological functions of miR-378 that involved in RCC. Methods　A total of 40 RCC tissues as well as 40 corresponding adjacent normal tissues were obtained from the Department of Urology, General Hospital of Eastern Theater Commend, PLA, between January 2014 and December 2018. In the meanwhile, serum samples from 60 RCC patients and 60 age-gender matched controls were also recruited from the department of clinical laboratory in the same hospital. Subsequently, TaqMan hydrolysis probe-based quantitative real-time PCR (RT-qPCR) was applied to determine the expression levels of miR-378 in the RCC tissues and serums, and the auxiliary diagnostic values of miR- 378 for RCC were evaluated by multiple statistical methods. Moreover, the biological functions of miR-378 that involved in RCC were also predicted and analyzed by bioinformatics’ tools. Results　RT-qPCR results showed that the relative expression levels of miR-378 were declined in RCC tissues [0.050 (0.016, 0.137)] as compared with adjacent normal tissues[0.134 (0.054, 0.546)]， the difference was statistically significant(Z=-3.481，P=0.003). Nevertheless, the expression levels of miR-378 in serum EVs were markedly elevated in RCC patients when compared with control subjects [(8.295 (4.930，15.60) vs 3.501 (2.005，6.853)]， the difference was statistically significant (U = 850, P ＜ 0.001). Receiver operating characteristic curve (ROC) analysis showed that the area under curve (AUC) for RCC discrimination was 0.665 (95% CI：0.544 ～ 0.786) for tissue miR- 378 and 0.764 (95% CI：0.680 ～ 0.848) for serum EV miR-378, respectively. Further bioinformatic analysis revealed that miR- 378 was mainly involved in transcriptional regulation, positive regulation of catabolic process, response to lipopolysaccharide, regulation of actin cytoskeleton, and regulation of protein kinase activity. Conclusion　The dysregulated expression levels of miR-378 in RCC tissues and sera EV have the potential as non-invasive biomarker for RCC auxiliary diagnosis, and miR-378 may involve in RCC occurrence and development through regulating multiple biological process.

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备注/Memo

备注/Memo:
基金项目：国家自然科学基金项目（81772282）。
作者简介：张明威（1988-），男，本科，检验技师，主要从事临床检验诊断学工作及研究，E-mail: 13110381@qq.com。
通讯作者：王成（1985-），男，博士，主管技师，主要从事临床生物化学与分子生物学检验及研究工作，E-mail: wangchengnju@163.com。
更新日期/Last Update: 1900-01-01