[1]任婷婷,杨艳娥,马歆花,等.支气管哮喘急性期患儿血清VAMP2 水平表达及其临床意义[J].现代检验医学杂志,2023,38(05):175-179.[doi:10.3969/j.issn.1671-7414.2023.05.033]
　REN Tingting,YANG Yane,MA Xinhua,et al.Expression and Clinical Significance of Serum VAMP2 in Children with Bronchial Asthma at Acute Stage[J].Journal of Modern Laboratory Medicine,2023,38(05):175-179.[doi:10.3969/j.issn.1671-7414.2023.05.033]
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支气管哮喘急性期患儿血清VAMP2 水平表达及其临床意义()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第38卷
期数:
2023年05期
页码:
175-179
栏目:
检验与临床
出版日期:
2023-09-15

文章信息/Info

Title:
Expression and Clinical Significance of Serum VAMP2 in Children with Bronchial Asthma at Acute Stage
文章编号:
1671-7414（2023）05-175-05
作者:
任婷婷; 杨艳娥; 马歆花; 王彦华
（西安医学院第二附属医院儿科，西安 710038）
Author(s):
REN Tingting ;  YANG Yan’e;  MA Xinhua;  WANG Yanhua
（Department of Pediatrics, the Second Affiliated Hospital of Xi’an Medical College, Xi’an 710038, China）
关键词:
支气管哮喘; 囊泡相关膜蛋白2; 肺功能
分类号:
R562.25；R392.11
DOI:
10.3969/j.issn.1671-7414.2023.05.033
文献标志码:
A
摘要:
目的 探讨支气管哮喘急性期患儿血清中囊泡相关膜蛋白2（vesicle-associated membrane protein 2，VAMP2）表达水平及其临床意义。方法 选取2017 年6 月～ 2020 年6 月在西安医学院第二附属医院儿科就诊的94 例支气管哮喘急性期患儿（研究组），以及同期入院复查的30 例支气管哮喘缓解期患儿（对照组）进行研究。比较两组的一般临床资料，并检查两组患儿的呼气峰流量（peak expiratory flow，PEF）、第1 秒用力呼气量（forced expiratory volume in 1second，FEV1）和用力肺活量（forced vital capacity，FVC）。采用酶联免疫吸附测定法（enzyme-linked immunosorbentassay，ELISA）检测血清VAMP2 水平，并根据支气管哮喘急性期患儿的严重程度分为轻度组（n=36）、中度组（n=31）和重度组（n=27），比较三组血清VAMP2 水平；支气管哮喘急性期患儿血清VAMP2 水平与PEF，FEV1，FVC 的关系采用Pearson 法分析；受试者工作特征（receiver operating characteristic，ROC）曲线分析血清VAMP2 水平对支气管哮喘急性期患儿发作的诊断价值。结果 研究组的血清VAMP2 水平（8.01±2.12 ng/L）高于对照组（5.23±1.21 ng/L），差异具有统计学意义（t=6.824，P<0.05）。研究组中的PEF（73.45%±7.12%），FEV1（65.34%±8.34%）和FVC（69.34%±9.14%）均低于对照组（87.34%±7.23%，82.45%±10.31%，89.89%±10.03%），差异具有统计学意义（t=9.269，9.222，10.471，均P<0.05）。重度组、中度组和轻度组血清VAMP2 水平依次为9.50±3.04ng/L，8.01±1.21 ng/L 和6.85±1.01 ng/L，差异具有统计学意义（F=15.412，P<0.05）；重度组VAMP2 水平高于中度组、轻度组（t=4.269，7.851，均P<0.05），中度组血清VAMP2 水平高于轻度组，差异具有统计学意义（t=3.571，P<0.05）。支气管哮喘急性期患儿血清VAMP2 水平与PEF，FEV1 和FVC 呈负相关（r=-0.506，-0.487，-0.399，均P<0.05）。血清VAMP2 水平诊断支气管哮喘急性期患儿发作的曲线下面积为0.909，截断值为6.802 ng/L，敏感度和特异度分别为77.7%，93.3%。结论 支气管哮喘急性期患儿血清VAMP2 水平升高，可以作为辅助诊断支气管哮喘急性发作的有效指标。
Abstract:
Objective To analyze the expression level of vesicle-associated membrane protein 2 (VAMP2) in serum of children with bronchial asthma at acute stage, and explore its clinical significance. Methods A total of 94 children with bronchial asthma at acute stage (study group) and 30 children with bronchial asthma in remission stage (control group) who were hospitalized in the Second Affiliated Hospital of Xi’an Medical College from June 2017 to June 2020 were selected for the study. The general clinical data of the two groups were compared, and the peak expiratory flow (PEF), forced expiratory volume in the first second (FEV1), forced vital capacity (FVC) of the two groups were examined. Serum VAMP2 levels were determined by enzyme-linked immunosorbent assay (ELISA), and the children with bronchial acute stage asthma were divided into mild group (n=36 ), moderate group (n=31) and severe group (n=27) according to the severity of acute stage. Pearson method was used to analyze the relationship between serum VAMP2 level, PEF, FEV1 and FVC in children with acute bronchial asthma. The diagnostic value of serum VAMP2 level in children with bronchial asthma at acute stage was analyzed by receiver operating characteristic (ROC) curve asthma. The diagnostic value of serum VAMP2 level in children with bronchial asthma at acute stage was analyzed by ROC curve. Results The serum VAMP2 level in the study group (8.01±2.12 ng/L) was higher than that in the control group (5.23±1.21 ng/L), and the difference was statistically significant (t=6.824, P<0.05). PEF (73.45%±7.12%), FEV1 (65.34%±8.34%) and FVC (69.34%±9.14%) in the study group were lower than those in the control group (87.34%±7.23%, 82.45%±10.31%, 89.89%±10.03%), and the differences were statistically significant (t=9.269, 9.222, 10.471, all P<0.05).Serum VAMP2 levels in severe group, moderate group and mild group were 9.50±3.04ng/L, 8.01±1.21 ng/L and 6.85±1.01 ng/L, respectively, and the difference was statistically significant (F=15.412，P<0.05), the VAMP2 level in the severe group was higher than that in the moderate group and the mild group (t= 4.269, 7.851, all P<0.05), the serum VAMP2 level in the moderate group was higher than that in the mild group, the difference was statistically significant (t=3.571, P<0.05). Serum VAMP2 level was negatively correlated with PEF, FEV1 and FVC in children with bronchial asthma at acute stage (r=-0.506, -0.487, -0.399, all P<0.05). The area under the curve of serum VAMP2 level in the diagnosis of children bronchial acute stage asthma was 0.909, the cut-off value was 6.802 ng/L, and the sensitivity and specificity were 77.7% and 93.3%, respectively. Conclusion The elevated serum VAMP2 level in children with the acute stage of bronchial asthma can be used as an effective indicator to help diagnose acute attack of bronchial asthma.

参考文献/References:

[1] 张洋, 侯伟. 儿童支气管哮喘心理行为问题研究进展[J]. 中国儿童保健杂志, 2019, 27(5): 523-526. ZHANG Yang, HOU Wei. Research progress on psychological and behavioral problems in children with bronchial asthma[J]. Chinese Journal of Child Health Care, 2019, 27(5): 523-526.
[2] MAHESH S, RAMAMURTHY M B. Management of acute asthma in children[J]. Indian Journal of Pediatrics, 2022, 89(4): 366-372.
[3] 郑明升, 彭东红. 2016-2018 年某院因支气管哮喘急性发作住院患儿危险因素分析[J]. 儿科药学杂志,2022, 28(3): 44-49. ZHENG Mingsheng, PENG Donghong. Risk factors of hospitalized children with acute attack of bronchial asthma in a hospital from 2016 to 2018[J]. Journal of Pediatric Pharmacy, 2022, 28(3): 44-49.
[4] 张颖, 娄欢, 田晓娜, 等. 环状RNA circ_0000285 通过吸附miR-127 调控VAMP2 水平参与卵巢癌进展[J]. 中华内分泌外科杂志, 2021, 15(5): 504-508. ZHANG Ying, LOU Huan, TIAN Xiaona, et al. Circ_0000285 regulates VAMP2 through sponging miR-127 and participates in the development of ovarian cancer [J]. Chinese Journal of Endocrine Surgery, 2021, 15(5): 504-508.
[5] DING Ling, LIU Guangling, LU Lu, et al. Circ_ CSNK1E modulates airway smooth muscle cells proliferation and migration via miR-34a-5p/VAMP2 axis in asthma [J]. Cell Signal, 2022, 95:110340.
[6] 中华医学会呼吸病学分会哮喘学组. 支气管哮喘防治指南( 支气管哮喘的定义、诊断、治疗及教育和管理方案)[J]. 中华结核和呼吸杂志,2003,26(3):132-138. Asthma Group of Chinese Thoracic Society. Guidelines for bronchial asthma(definition, diagnosis, treatment, education and management of bronchial asthma)[J]. Chinese Journal of Tuberculosis and Respiratory Diseases, 2003, 26(3): 132-138.
[7] WANG Wenlan, LUO Xiaoming, ZHANG Qin, et al. The LncRNA PVT1/miR-590-5p/FSTL1 axis modulates the proliferation and migration of airway smooth muscle cells in asthma[J]. Autoimmunity, 2021, 54(3): 138-147.
[8] 黄鹤. 清肺定喘方加减辨治小儿支气管哮喘急性期的效果[J]. 中国医药指南, 2020, 18(11): 172-173. HUANG He. Effect of Qingfei Dingchuan decoction on acute stage of pediatric bronchial asthma[J]. Guide of China Medicine, 2020, 18(11): 172-173.
[9] HAKTANIR ABUL M, PHIPATANAKUL W. Severe asthma in children: Evaluation and management[J].Allergology International, 2019, 68(2): 150-157.
[10] LAM M, TAKEO K, ALMEIDA R G, et al. CNS myelination requires VAMP2/3-mediated membrane expansion in oligodendrocytes[J]. Nature Communications, 2022, 13(1): 5583.
[11] OROCK A, LOGAN S, DEAK F. Age-related cognitive impairment:role of reduced synaptobrevin-2 levels in deficits of memory and synaptic plasticity[J]. J Gerontol A Biol Sci Med Sci, 2020, 75(9): 1624-1632.
[12] RAJA S A, ABBAS S, SHAH S T A, et al. Increased expression levels of syntaxin 1A and Synaptobrevin 2/vesicle-associated membrane protein-2 are associated with the progression of bladder cancer[J]. Genetics and Molecular Biology, 2019, 42(1): 40-47.
[13] YAN Chong, JIANG Jie, YANG Yuan, et al. The function of VAMP2 in mediating membrane fusion: An overview[J]. Front Mol Neurosci, 2022, 15:948160.
[14] XU Xiaojun , ZHANG Xiaoying , ZHANG Shen ,et al. Bone marrow mesenchymal stem cell with upregulation of microRNA-206 in the treatment of bronchopulmonary dysplasia in newborn mice[J].Iranian Journal of Pediatrics, 2019, 29(3)：e11121.
[15] CHOI Y, JANG J, PARK H S. Pulmonary surfactants: a new therapeutic target in asthma[J]. Current Allergy and Asthma Reports, 2020, 20(11): 70.
[16] TANG B L. SNAREs and developmental disorders[J].Journal of Cellular Physiology, 2021, 236(4): 2482-2504.
[17] CARDENAS R A, GONZALEZ R, SANCHEZ E, et al. SNAP23 is essential for platelet and mast cell development and required in connective tissue mast cells for anaphylaxis [J]. J Biol Chem, 2021, 296:100268.
[18] LAU T S, CHAN L K Y, MAN G C W, et al. Paclitaxel induces immunogenic cell death in ovarian cancer via TLR4/IKK2/SNARE-Dependent exocytosis[J]. Cancer Immunol Res, 2020, 8(8): 1099-1111.
[19] 杨明新, 姚梦, 魏名一, 等. 支气管哮喘患儿血清半乳糖凝集素3、类胰蛋白酶、25- 羟维生素D3 与肺功能和生活质量的相关性分析[J]. 现代生物医学进展, 2022, 22(6): 1112-1115, 1120. YANG Mingxin, YAO Meng, WEI Mingyi, et al. Correlation analysis of serum galectin 3,tryptase,25-hydroxyvitamin D3 and lung function and quality of life in children with bronchial asthma[J]. Progress in Modern Biomedicine, 2022, 22(6): 1112-1115, 1120.
[20] 李颖, 任炳臣, 韩晓庆, 等. 支气管哮喘患者血清microRNA-145 水平表达与肺功能、 气道重塑及Th1/Th2 平衡的关系分析[J]. 现代检验医学杂志,2021, 36(5): 133-137, 179. LI Ying, REN Bingchen, HAN Xiaoqing, et al. Relationship between serum microRNA-145 expression with lung function, airway remodeling and Th1/Th2 Balance in patients with bronchial asthma[J]. Journal of Modern Laboratory Medicine, 2021, 36(5): 133-137, 179.
[21] 李晓刚, 邓巧妮, 王军焕, 等. 儿童哮喘患者外周血ILC2 细胞和Th2 型细胞相关因子水平变化及与临床相关性研究[J]. 现代检验医学杂志, 2020, 35(6):72-77. LI Xiaogang, DENG Qiaoni, WANG Junhuan, et al. Changes of ILC2 cells and Th2 cell related factors in peripheral blood of children with asthma and their clinical relevance[J]. Journal of Modern Laboratory Medicine, 2020, 35(6): 72-77.

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备注/Memo

备注/Memo:
作者简介：任婷婷（1986-），女，硕士研究生，主治医师，研究方向：哮喘，E-mail：rdkjb66@163.com。
通讯作者：王彦华（1989-），女，硕士研究生，主治医师，研究方向：哮喘，E-mail：791745435@qq.com。
更新日期/Last Update: 2023-09-15