[1]王鹏飞a,陈长英a,靳玉娟b,等.阿尔茨海默症患者血清miR-211 和miR-202 表达水平及其与认知功能、焦虑抑郁情绪的相关性研究[J].现代检验医学杂志,2024,39(02):129-134.[doi:10.3969/j.issn.1671-7414.2024.02.024]
　WANG Pengfeia,CHEN Changyinga,JIN Yujuanb,et al.Serum miR-211 and miR-202 Expression Levels in Alzheimer’s Disease Patients and Their Correlation with Cognitive Function, Anxiety and Depression[J].Journal of Modern Laboratory Medicine,2024,39(02):129-134.[doi:10.3969/j.issn.1671-7414.2024.02.024]
点击复制

阿尔茨海默症患者血清miR-211 和miR-202 表达水平及其与认知功能、焦虑抑郁情绪的相关性研究()
分享到：

《现代检验医学杂志》[ISSN:/CN:]

卷:
第39卷
期数:
2024年02期
页码:
129-134
栏目:
论著
出版日期:
2024-03-31

文章信息/Info

Title:
Serum miR-211 and miR-202 Expression Levels in Alzheimer’s Disease Patients and Their Correlation with Cognitive Function, Anxiety and Depression
文章编号:
1671-7414（2024）02-129-06
作者:
王鹏飞a; 陈长英a; 靳玉娟b; 孙安龙c
（河北燕达医院 a. 神经内科；b. 急诊内科；c. 老年医学科，河北廊坊 065401）
Author(s):
WANG Pengfeia;  CHEN Changyinga;  JIN Yujuanb;  SUN Anlongc
（a. Department of Neurology; b. Department of Emergency Internal Medicine; c. Department of Geriatrics, Hebei Yanda Hospital, Hebei Langfang 065401, China）
关键词:
阿尔茨海默症; 微小核糖核酸-211; 微小核糖核酸-202; 认知功能; 焦虑抑郁情绪
分类号:
R749.16；R392.11
DOI:
10.3969/j.issn.1671-7414.2024.02.024
文献标志码:
A
摘要:
目的 分析阿尔茨海默症（Alzheimer’s disease）患者血清微小核糖核酸（microRNA，miR）-211 和miR-202表达水平及与认知功能、焦虑抑郁情绪的相关性研究。方法 选取2019 年3 月～ 2022 年3 月河北燕达医院收治的阿尔茨海默症患者90 例作为研究组，根据临床痴呆评定量表（clinical dementia rating，CDR）评分将患者分为轻度组（n=24）、中度组（n=48）和重度组（n=18）。另选取同期健康体检者90 例作为对照组，比较血清miR-211 和miR-202 表达水平。Pearson 法和Spearman 法分析血清miR-211，miR-202 以及二者与认知功能、焦虑抑郁情绪的相关性，采用Logistic 回归分析阿尔茨海默症的影响因素。结果 与对照组比较，研究组血清miR-211（0.59 ± 0.16 vs 1.01 ± 0.31）、miR-202（0.35± 0.10 vs 1.00 ± 0.32）表达水平降低，差异具有统计学意义（t=11.422，18.393，均P ＜ 0.05）。轻度、中度和重度组血清miR-211（0.73 ± 0.21，0.62 ± 0.17，0.32 ± 0.08）表达水平、miR-202（0.51 ± 0.15，0.33 ± 0.10，0.19 ± 0.04）表达水平、简易精神状态检查量表（mini-mental state examination，MMSE）评分（22.54 ± 1.41 分，19.35 ± 1.01 分，16.23 ± 1.00 分）和蒙特利尔认知评估量表（montreal cognitive assessment，MoCA）评分（25.35 ± 2.60 分，18.59 ± 1.32 分，16.59 ± 1.24 分）逐渐降低，差异具有统计学意义（F=32.006，46.917，163.048，163.703，均P ＜ 0.05）。与轻度组比较，重度组、中度组血清miR-211，miR-202，MMSE和MoCA评分降低，差异具有统计学意义（t=3.685~25.375，均P ＜ 0.05）。轻度、中度和重度组Hamilton 焦虑量表（hamilton anxiety scale，HAMA）评分（12.34 ± 1.27 分，20.59 ± 2.09 分，31.29 ± 2.19 分）和Hamilton 抑郁量表（hamilton depression scale，HAMD）评分（14.35 ± 2.13 分，23.89 ± 2.20 分，35.35 ± 1.21 分）逐渐升高，差异具有统计学意义（F=496.059，553.939，均P ＜ 0.05）。根据Pearson 相关性分析得知，miR-211 与miR-202 呈正相关（r=0.615，P ＜ 0.05）。根据Spearman 相关性分析得知，miR-211 和miR-202 与MMSE和MoCA 呈显著正相关（r=0.539 ～ 0.585，均P ＜ 0.05）；与HAMA 和HAMD 呈显著负相关（r=-0.651 ～ -0.539，均P ＜ 0.05）。根据Logistic 回归分析得知miR-211[OR（95%CI）：5.321（1.648 ～ 17.180）] 和miR-202[OR（95%CI）：3.158（1.989 ～ 5.012）] 低表达是影响阿尔茨海默症的危险因素（均P ＜ 0.05）。结论 阿尔茨海默症患者血清miR-211 和miR-202 表达水平降低，且miR-211 和miR-202 与认知功能、焦虑抑郁情绪密切相关。
Abstract:
Objective To analyze the expression levels of serum microRNA (miR)-211 and miR-202 in patients with Alzheimer’s disease and their correlation with cognitive function, anxiety and depression. Methods A total of 90 patients with Alzheimer’s disease admitted to Hebei Yanda Hospital from March 2019 to March 2022 were selected as the research group. According to the Clinical Dementia Rating (CDR) score, the patients were grouped into mild group (n=24), moderate group (n=48) and severe group (n=18). Another 90 healthy individuals who underwent physical examination were collected as the control group. The expression levels of miR-211 and miR-202 in serum were compared. Pearson method and Spearman method were used to analyze serum miR-211 and miR-202 and their correlation with cognitive function, anxiety and depression. Logistic regression analysis was used to analyze the influencing factors of Alzheimer’s disease. Results The expression levels of serum miR-211 (0.59 ± 0.16, 1.01 ± 0.31) and miR-202 (0.35 ± 0.10, 1.00 ± 0.32) were significantly reduced in the research group and control group, with significant differences (t=11.422, 18.393, all P<0.05). Serum miR-211 (0.73 ± 0.21，0.62 ± 0.17，0.32 ± 0.08) expression levels, miR-202 (0.51±0.15, 0.33±0.10，0.19±0.04) expression levels, mini-mental state examination (MMSE) score (22.54±1.41 score, 19.35 ± 1.01 score，16.23 ± 1.00 score) and Montreal cognitive assessment (MoCA) score (25.35±2.60 score，18.59±1.32 score，16.59±1.24 score) in the mild, moderate and severe groups gradually decreased, and the differences were statistically significant (F=32.006, 46.917, 163.048, 163.703, all P<0.05). Compared with mild group, the serum miR-211, miR-202, MMSE and MoCA scores of severe group and moderate group were reduced, and the differences were statistically significant (t=3.685~25.375, all P<0.05). The mild, moderate and severe groups had a gradual increase in Hamilton anxiety scale (HAMA) score (12.34±1.27 score, 20.59±2.09 score and 31.29 ± 2.19 score) and Hamilton depression scale (HAMD) score (14.35±2.13 score, 23.89 ± 2.20 score and 35.35 ± 1.21 score), and the differences were statistically significant (F=496.059, 553.939, all P<0.05). According to Pearson correlation analysis, miR-211 was positively correlated with miR-202 (r=0.651, P<0.05). According to Spearman correlation analysis, miR-211 and miR-202 were positively correlated with MMSE and MoCA (r=0.539 ～ 0.585, all P<0.05) and negatively correlated with HAMA and HAMD (r=-0.651 ～ -0.539, all P<0.05). Logistic regression analysis showed that the low expression of miR-211[OR (95%CI)：5.321 (1.648 ～ 17.180)] and miR- 202[OR (95%CI)：3.158 (1.989 ～ 5.012)] were risk factors for Alzheimer’s disease (P<0.05). Conclusion The serum expression levels of miR-211 and miR-202 in patients with Alzheimer’s disease were reduced, indicating miR-211 and miR-202 were closely related to cognitive function, anxiety and depression.

参考文献/References:

[1] 孟凯涛, 张建国, 刘崇, 等. 不同严重程度阿尔茨海默病患者血清miR-128, miR-223 表达水平变化与炎症反应及认知功能的相关性分析[J]. 卒中与神经疾病, 2021, 28(6): 667-671. MENG Kaitao, ZHANG Jianguo, LIU Chong, et al. The correlation between the expression of serum miR-128 and miR-223 and inflammatory response and cognition in patients with different severity of Alzheimer's disease[J]. Stroke and Neurological Diseases, 2021, 28(6): 667-671.
[2] CHHETRI J K, CHAN P, VELLAS B, et al. Report from the first clinical trials on Alzheimer’s disease (CTAD) Asia-china 2018: bringing together Global Leaders[J]. Journal of Prevention of Alzheimer’s Disease, 2019, 6(2): 144-147.
[3] 郭云红, 王郁涛, 从恩朝, 等. 低流量鼻导管吸氧联合多奈哌齐治疗阿尔茨海默病患者抑郁、焦虑情绪的疗效观察[J]. 上海医药, 2023, 44(1): 36-39. GUO Yunhong, WANG Yutao, CONG Enchao, et al. Study of the effect of low flow nasal catheter oxygen inhalation combined with donepezil on depression and anxiety in patients with Alzheimer’s disease[J].Shanghai Medical & Pharmaceutical Journal, 2023,44(1): 36-39.
[4] 朱振霞, 于浩, 谢文丽. 阿尔茨海默症发病机制及治疗药物研究进展[J]. 武警后勤学院学报( 医学版),2021, 30(5): 146-149. ZHU Zhenxia, YU Hao, XIE Wenli. Research progress in the pathogenesis and drug therapy of Alzheimer’s disease[J]. Journal of Logistics University of PAP(Medical Sciences), 2021, 30(5): 146-149.
[5] LIU Wenyi, MIAO Yuanqing, ZHANG Lin, et al. MiR-211 protects cerebral ischemia/reperfusion injury by inhibiting cell apoptosis[J]. Bioengineered, 2020,11(1): 189-200.
[6] 徐沛沛, 赵树华, 王江波, 等. 过表达miR-202-5p 通过抑制PCSK9 减轻阿尔茨海默病神经损伤[J]. 中国比较医学杂志, 2022, 32(10): 49-58. XU Peipei, ZHAO Shuhua, WANG Jiangbo, et al. Overexpression of miR-202-5p attenuates nerve damage in Alzheimer’s disease by inhibiting PCSK9[J].Chinese Journal of Comparative Medicine, 2022,32(10): 49-58.
[7] 中华医学会精神病学分会. 中国精神障碍分类与诊断标准第三版( 精神障碍分类)[J]. 中华精神科杂志,2001, 34(3): 184-188. Chinese Society of Psychiatry, Chinese Medical Association. Classification and diagnostic criteria of mental disorders in China-Third edition (classification of mental disorders)[J]. Chinese Journal of Psychiatry,2001, 34(3): 184-188.
[8] 孙祝平, 陈红英, 陈思路. 老年阿尔茨海默病患者血清Lp-PLA2, NLRP3 水平表达及其与认知功能损害的相关性[J]. 现代检验医学杂志, 2020, 35(1): 49-52. SUN Zhuping, CHEN Hongying, CHEN Silu. Expression of serum Lp-PLA2 and NLRP3 in elderly patients with Alzheimer’s disease and its relationship with cognitive impairment[J]. Journal of Modern Laboratory Medicine, 2020, 35(1): 49-52.
[9] 薛斌, 秦杰, 俞芳, 等. 医养结合康复模式对阿尔茨海默病患者日常生活活动能力及焦虑抑郁情绪的影响[J]. 上海医药, 2022, 43(4): 59-62. XUE Bin, QIN Jie, YU Fang, et al. Effects of rehabilitation mode of combining medical treatment and elderly care on daily living ability, anxiety and depression in patients with Alzheimer’s disease[J].Shanghai Medical & Pharmaceutical Journal, 2022,43(4): 59-62.
[10] 付劭静, 杨月明, 周延华, 等. 阿尔茨海默症患者血清缓激肽,s100β 蛋白和Aβ1-42 表达水平及与患者神经功能、认知功能的关系[J]. 河北医药, 2022,44(15): 2245-2249. FU Shaojing, YANG Yueming, ZHOU Yanhua, et al. Correlation between the expression levels of serum bradykinin, s100β protein, Aβ1-42 and the neurological function, cognitive function of patients with Alzheimer’s disease[J]. Hebei Medical Journal,2022, 44(15): 2245-2249.
[11] 尹长林, 余永强, 田仰华, 等. 阿尔茨海默病患者精神行为症状与海马及其亚区灰质体积关系的研究[J]. 安徽医药, 2019, 40(10): 1087-1090. YIN Changlin, YU Yongqiang, TIAN Yanghua, et al. A study of neuropsychiatric syndrome in Alzheimer’s disease and its relationship with gray matter volume of hippocampus and hippocampus sub-region[J]. Anhui Medical Journal, 2019, 40(10): 1087-1090.
[12] 张莹林, 姚俊岩. 神经炎症在阿尔获海默病中作用的研究进展[J]. 国际麻醉学与复苏杂志, 2022,43(1): 90-94. ZHANG Yinglin, YAO Junyan. Research advances of the role of neuroinflammation in Alzheimer’s disease[J]. International Journal of Anesthesiology and Resuscitation, 2022, 43(1): 90-94.
[13] 薛玉峰, 叶丽君, 傅攀, 等. miR-135a 和miR-146a在阿尔茨海默病患者中的表达及诊断效能评价[J].中国医药导报, 2020, 17(23): 16-19. XUE Yufeng, YE Lijun, FU Pan, et al. Expression and diagnostic efficacy of miR-135a and miR-146a in patients with Alzheimer’s disease[J]. China Medical Herald, 2020, 17(23): 16-19.
[14] LI Enyao, ZHAO Pengju, JIAN Jie, et al. LncRNA MIAT overexpression reduced neuron apoptosis in a neonatal rat model of hypoxic-ischemic injury through miR-211/GDNF[J]. Cell Cycle, 2019, 18(2): 156-166.
[15] LI Liping, MIAO Miao, CHEN Jiarui, et al. Role of ten eleven translocation‐2 (Tet2) in modulating neuronal morphology and cognition in a mouse model of Alzheimer’s Disease[J]. Journal of Neurochemistry,2021, 157(4): 993-1012.
[16] LIU Xinhong, NING Fangbo, ZHAO Dapeng, et al. Role of miR-211 in a PC12 cell model of Alzheimer’s disease via regulation of neurogenin 2[J]. Experimental Physiology, 2021, 106(4): 1061-1071.
[17] LI Ye, FAN Cuiqin, GAO Rui, et al. Hippocampal miR-211-5p regulates neurogenesis and depression-like behaviors in the rat[J]. Neuropharmacology, 2021, 194:108618.
[18] 孙亚云, 苏建华, 张俊华, 等. miR-211-5p 在局灶性脑缺血再灌注损伤大鼠模型中表达分析[J]. 解放军医药杂志, 2022, 34(5): 5-8. SUN Yayun, SU Jianhua, ZHANG Junhua, et al.Expression analysis of miR-211-5p in a rat model of focal cerebral ischemia-reperfusion injury[J].Medical & Pharmaceutical Journal of Chinese People’s Liberation Army, 2022, 34(5): 5-8.
[19] 李男, 高关羽, 聂桐. 木犀草苷通过调控miR-211 表达对缺氧缺糖诱导的大鼠皮质神经细胞损伤的影响[J]. 中国药师, 2021, 24(5): 839-844. LI Nan, GAO Guanyu, NIE Tong. Effects of luteolin on oxygen-glucose deprivation induced cortical nerve cell damage in rats by regulating miR-211 expression[J].Chinese Pharmacist, 2021, 24(5): 839-844.
[20] LI Bing, HUANG Zhi, MENG Ju, et al. MiR-202-5p attenuates neurological deficits and neuronal injury in MCAO model rats and OGD-induced injury in Neuro-2a cells by targeting eIF4E-mediated induction of autophagy and inhibition of Akt/GSK-3β pathway[J].Molecular and Cellular Probes, 2020, 51: 101497.
[21] DONG Lihua, SUN Lei, ZHANG Wenjing, et al. Reduced serum miR-202 may promote the progression of Alzheimer’s disease patients via targeting amyloid precursor protein[J]. Kaohsiung Journal of Medical Sciences, 2021, 37(8): 730-738.
[22] XIN Cuiyu, XIA Jiejing, LIU Yulan, et al. MicroRNA-202-3p targets brain-derived neurotrophic factor and is involved in depression-like behaviors[J].Neuropsychiatric Disease and Treatment, 2020, 16:1073-1083.

相似文献/References:

[1]张姝,罗洁,许琦,等.贵州地区汉族阿尔茨海默症患者CYP2C19 基因多态性及代谢表型分析[J].现代检验医学杂志,2020,35(05):13.[doi:10.3969/j.issn.1671-7414.2020.05.004]
　ZHANG Shu,LUO Jie,XU Qi,et al.Analysis of CYP2C19 Gene Polymorphism and Metabolic Phenotype in Alzheimerdisease Patients of Han Nationality in Guizhou[J].Journal of Modern Laboratory Medicine,2020,35(02):13.[doi:10.3969/j.issn.1671-7414.2020.05.004]

备注/Memo

备注/Memo:
基金项目： 2022 年廊坊市科学技术研究与发展计划自筹经费项目（2022013005）：社区老年人群焦虑抑郁量表联合认知评估量表与痴呆的相关性及影响因素研究。
作者简介：王鹏飞（1978-），男，硕士，副主任医师，研究方向：脑血管病，E-mail：13803162374@163.com。
更新日期/Last Update: 2024-03-15