[1]张 群,刘 婕,谢艳飞.寻常性银屑病患者血清miR-133a-3p 和PTPN22 水平表达与疾病严重程度的相关性分析[J].现代检验医学杂志,2024,39(02):135-139.[doi:10.3969/j.issn.1671-7414.2024.02.025]
　ZHANG Qun,LIU Jie,XIE Yanfei.Correlation Analysis between Serum miR-133a-3p and PTPN22 Levels Expression and Disease Severity in Patients with Psoriasis Vulgaris[J].Journal of Modern Laboratory Medicine,2024,39(02):135-139.[doi:10.3969/j.issn.1671-7414.2024.02.025]
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寻常性银屑病患者血清miR-133a-3p 和PTPN22 水平表达与疾病严重程度的相关性分析()
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《现代检验医学杂志》[ISSN:/CN:]

卷:
第39卷
期数:
2024年02期
页码:
135-139
栏目:
论著
出版日期:
2024-03-31

文章信息/Info

Title:
Correlation Analysis between Serum miR-133a-3p and PTPN22 Levels Expression and Disease Severity in Patients with Psoriasis Vulgaris
文章编号:
1671-7414（2024）02-135-05
作者:
张 群; 刘 婕; 谢艳飞
（沧州市人民医院皮肤科，河北沧州 061000）
Author(s):
ZHANG Qun;  LIU Jie;  XIE Yanfei
(Department of Dermatology, Cangzhou People’s Hospital, Hebei Cangzhou 061000, China)
关键词:
寻常性银屑病; 微小RNA-133a-3p; 蛋白酪氨酸磷酸酶非受体型22; 疾病严重程度
分类号:
R758.63；R392.11
DOI:
10.3969/j.issn.1671-7414.2024.02.025
文献标志码:
A
摘要:
目的 探究寻常性银屑病患者血清微小RNA（microRNA，miR）-133a-3p，蛋白酪氨酸磷酸酶非受体型22（protein tyrosine phosphatase non-receptor type 22，PTPN22）水平表达与疾病严重程度的相关性。方法 收集沧州市人民医院2022 年1 ～ 6 月收治的寻常性银屑病患者86 例作为观察组，根据皮损面积和严重程度将其分为进展期组（n=41）和静止期组（n=45），同期选取整形外科体检健康者86 例作为对照组。采用实时荧光定量PCR（real time fluorescentquantitative PCR，qRT-PCR）法检测患者血清miR-133a-3p 和PTPN22 相对表达水平；Target Scan Human 网站预测PTPN22 与miR-133a-3p 的靶向关系；运用Spearman 法分析寻常性银屑病患者血清miR-133a-3p，PTPN22 表达水平与银屑病皮损面积及严重程度指数（the psoriasis area and severity index score，PASI）评分的相关性；行Logistic 回归分析寻常性银屑病患者严重度的影响因素。结果 与对照组相比，观察组血清miR-133a-3p（1.85 ± 0.46 vs 1.05 ± 0.21）表达水平明显升高，PTPN22 mRNA（0.76 ± 0.13 vs 1.02 ± 0.18）表达水平明显降低，差异具有统计学意义（t=14.671，10.859，均P ＜ 0.05）；与静止期组比较，进展期组血清miR-133a-3p（2.05 ± 0.52 vs 1.67 ± 0.41）表达水平明显升高，PTPN22 mRNA（0.66 ± 0.11 vs 0.85 ± 0.15）表达水平明显降低，差异具有统计学意义（t=3.780，6.643，均P ＜ 0.05）。Target Scan Human 网站预测，miR-133a-3p 与PTPN22 可能存在靶向关系。Spearman 分析显示，寻常性银屑病患者血清miR-133a-3p 与PASI 评分呈正相关性（r=0.469，P＜ 0.05），而血清PTPN22 mRNA水平与PASI 评分呈负相关性（r=-0.508，P ＜ 0.05）；血清miR-133a-3p[OR(95%CI)=2.884(1.261~6.595)] 是寻常性银屑病患者严重度的独立危险因素，而PTPN22[OR(95%CI)=0.562(0.367~0.860)] 是独立保护因素（均P ＜ 0.05）。结论 寻常性银屑病患者血清miR-133a-3p 表达水平明显升高，PTPN22 表达水平明显降低，两者与PASI 评分紧密相关，且在一定程度上可反映银屑病患者的严重程度。
Abstract:
Objective To explore the correlation between the serum levels expression of microRNA(miR)-133a-3p, protein tyrosine phosphatase nonreceptor type 22 (PTPN22) and the severity of psoriasis vulgaris. Methods A total of 86 patients with psoriasis vulgaris who were admitted to Cangzhou People’s Hospital from January 2022 to June 2022 were collected as the observation group. They were separated into a progressive group (n=41) and a quiescent group (n=45) based on the area and severity of the skin lesions. Meantime, 86 healthy individuals undergoing plastic surgery examinations were regarded as the control group. Real time fluorescent quantitative PCR (qRT-PCR) method was applied to detect the relative expression levels of miR-133a-3p and PTPN22 in serum. Target Scan Human website was applied to predict the targeting relationship between PTPN22 and miR-133a-3p. Spearman method was applied to analyze the correlation between the expression levels of miR-133a- 3p and PTPN22 in serum of patients with psoriasis vulgaris, the psoriasis area and the psoriasis area and severity index score (PASI). Logistic regression was applied to analyze the influencing factors of severity in patients with psoriasis vulgaris. Results Compared with the control group, the serum miR-133a-3p (1.85 ± 0.46 vs 1.05 ± 0.21) expression level in the observation group was increased, while the PTPN22 mRNA (0.76 ± 0.13 vs 1.02 ± 0.18) expression level was reduced, and the difference were statistically significant (t=14.671, 10.859, all P<0.05). Compared with the quiescent group, the serum miR-133a-3p (2.05 ± 0.52 vs 1.67 ± 0.41) expression level in the progressive group was increased, while the PTPN22 mRNA (0.66 ± 0.11 vs 0.85 ± 0.15) expression level was reduced and the differences were statistically significant (t=3.780, 6.643, all P<0.05). Target Scan Human website predicted that there may be a targeting relationship between miR-133a-3p and PTPN22. Spearman analysis showed that there was a positive correlation between serum miR-133a-3p and PASI score in patients with psoriasis vulgaris(r=0.469, P<0.05), while serum TPN22 mRNA level was negatively correlated with PASI score (r=0.469，P<0.05). Serum miR-133a-3p [OR(95%CI)=2.884(1.261~6.595)] was an independent risk factor for the severity of psoriasis vulgaris, while PTPN22 [OR(95%CI)=0.562(0.367~0.860)] was an independent protective factor (all P<0.05). Conclusion The expression level of miR-133a-3p in serum of patients with psoriasis vulgaris was increased, while the expression level of PTPN22 was reduced. The two were closely related to the PASI score and may to some extent reflect the severity of psoriasis patients.

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备注/Memo

备注/Memo:
基金项目：河北省2022 年度医学科学研究课题计划（20220306）：银屑病外周血及皮损内miRNA-138 与银屑病活动性研究。
作者简介：张群（1985-），男，硕士研究生，主治医师，研究方向：皮肤病以及皮肤美容 ，E-mail：a8bqh0@163.com。
通讯作者：谢艳飞（1975-），男，本科，副主任医师，研究方向：银屑病、皮肤病理，E-mail：chuguanna11@163.com。
更新日期/Last Update: 2024-03-15